Differentiation of Human GBM From Non-GBM Brain Tissue With Polarization Imaging Technique

Abstract

As for optical techniques, it is difficult for the 5-aminolevulinic (5-ALA) fluorescence guidance technique to completely detect glioma due to residual cells in the blind area and the dead angle of vision under microscopy. The purpose of this research is to characterize different microstructural information and optical properties of formalin-soaked unstained glioblastoma (GBM) and non-GBM tissue with the polarization imaging technique (PIT), and provide a novel method to detect GBM during surgery. In this paper, a 3×3 Mueller matrix polarization experimental system in backscattering mode was built to detect the GBM and non-GBM tissue bulk. The Mueller matrix decomposition and transformation parameters of GBM and non-GBM tissue were calculated and analyzed, and showed that parameters (1−Δ) and t are good indicators for distinguishing GBM from non-GBM tissues. Furthermore, the central moment coefficients (CMCs) of the frequency distribution histogram (FDH) were also calculated and used to distinguish the cancerous tissues. The results of the experiments confirmed the feasibility of PIT applied in the clinic to detect glioma, laying the foundation for the subsequent non-invasive, non-staining glioma detection.

A polarization image enhancement method for glioma

“…It is based on the analysis of the modification of the polarization state of incident polarized light due to the interaction with the sample to be examined, which can be described by the Mueller matrix. In our previous work, it was confirmed that PIT can investigate optical information and microstructures of glioma and non-glioma tissues from clinical treatment based on backward scattering 3 × 3 Mueller matrix polarization imaging experimental setup, and the polarization properties of the glioma and non-glioma brain tissues have been characterized and reported ( Liu et al, 2022 ).…”

Polarization image fusion method with image enhancement

“…The polarization imaging technique obtains the target's intrinsic properties by acquiring the degree of linear polarization (DoLP) image and the angle of polarization (AoP) image. As an advanced technology, it can not only obtain the polarization information of the target, but also provide the characteristics of light intensity distribution in two-dimensional space, which has a wide range of potential applications in many fields such as target detection [2,3], communication [4], underwater detection [5], and medical imaging [6].…”