Differentiation of human adult cardiac stem cells exposed to extremely low-frequency electromagnetic fields

Gaetani, Roberto; Ledda, Mario; Barile, Lucio; Chimenti, Isotta; Carlo, Flavia De; Forte, Elvira; Ionta, Vittoria; Giuliani, Livio; D’Emilia, Enrico; Frati, Giacomo; Miraldi, Fabio; Pozzi, Deleana; Messina, Elisa; Grimaldi, Settimio; Giacomello, Alessandro; Lisi, Antonella

doi:10.1093/cvr/cvp067

Cited by 104 publications

(60 citation statements)

References 33 publications

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“…These included rat immature cerebellar granule neurons and mouse embryonic neural stem cells [3, 41], as well as mouse pituitary corticotrope-derived cells [42]. Similar results were obtained in human cardiac stem cells derived from human myocardial bioptic specimens, where differentiation toward a cardiac-specific phenotype was observed after exposure to low-frequency EMF [43]. Interestingly, ELF-EMF exposure was recently seen to promote differentiation also in human cells from hematopoietic malignancies, such as the myelogenous leukemia cell line K562 [44].…”

Section: Discussionmentioning

confidence: 68%

Effects of Fifty-Hertz Electromagnetic Fields on Granulocytic Differentiation of ATRA-Treated Acute Promyelocytic Leukemia NB4 Cells

Provenzano

Amatori

Nasoni

et al. 2018

Cell Physiol Biochem

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Background/Aims: Life on Earth is constantly exposed to electromagnetic fields (EMFs) and the effects induced by EMFs on biological systems have been extensively studied producing different and sometimes contradictory results. Extremely low-frequency electromagnetic fields (ELF-EMFs) have shown to play a role in regulating cell proliferation and differentiation, although how EMFs influence these processes remains unclear. Human acute promyelocytic leukemia (APL) cells are characterized by the arrest of differentiation at the promyelocytic stage due to epigenetic perturbations induced by PML/RARα fusion protein (Promyelocytic Leukemia protein - PML/Retinoic Acid Receptor alpha - RARα). Therapeutic administration of all-trans retinoic acid (ATRA) re-establishes the leukemogenic mechanism re-inducing the normal differentiation processes. Methods: We studied the effects of ELF-EMFs (50 Hz, 2 mT) on the ATRA-mediated granulocytic differentiation process of APL NB4 cells (a cell line established from the bone marrow of a patient affected by the acute promyelocytic leukemia) by monitoring cellular proliferation and morphology, nitrob lue tetrazolium (NBT) reduction and the expression of differentiation surface markers. Finally, we investigated mechanisms focusing on reactive oxygen species (ROS) generation and related molecular pathways. Results: ELF-EMF exposure decreases cellular proliferation potential and helps ATRA-treated NB4 cells to mature. Furthermore, the analysis of ROS production and the consequent extracellular signal regulated kinases (ERK1/2) phosphorylation suggest that a changed intracellular oxidative balance may influence the biological effects of ELF-EMFs. Conclusions: These results indicate that the exposure to ELF-EMF promotes ATRA-induced granulocytic differentiation of APL cells.

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Section: Discussionmentioning

confidence: 68%

Effects of Fifty-Hertz Electromagnetic Fields on Granulocytic Differentiation of ATRA-Treated Acute Promyelocytic Leukemia NB4 Cells

Provenzano

Amatori

Nasoni

et al. 2018

Cell Physiol Biochem

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“…We chose 5 mT because common daily exposure to LF EMF will mainly be experienced in comparable field strengths, ranging from 0.07 mT for average residential power-frequency magnetic fields in homes in Europe, to about 20 mT under power lines [WHO, 2007]. Moreover, effects of fields with similar strengths have been found, such as differentiation in adult cardiac progenitor cells exposed to 2.5 and 10 mT fields [Gaetani et al, 2009]. The complex IM signal has shown interesting results using this low magnetic field strength.…”

Section: Discussionmentioning

confidence: 99%

Low‐frequency electromagnetic fields do not alter responses of inflammatory genes and proteins in human monocytes and immune cell lines

Bouwens

Kleijn

Ferwerda

et al. 2011

Bioelectromagnetics

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The effects of low frequency electromagnetic fields (LF EMF) on human health are the subject of on-going research and serious public concern. These fields potentially elicit small effects that have been proposed to have consequences, either positive or negative, for biological systems. To reveal potentially weak but biologically relevant effects, we chose to extensively examine exposure of immune cells to two different signals, namely a complex multiple waveform field, and a 50 Hz sine wave. These immune cells are highly responsive and, in vivo, modulation of cytokine expression responses can result in systemic health effects. Using time course experiments, we determined kinetics of cytokine and other inflammation-related genes in a human monocytic leukemia cell line, THP-1, and primary monocytes and macrophages. Moreover, cytokine protein levels in THP-1 monocytes were determined. Exposure to either of the two signals did not result in a significant effect on gene and protein expression in the studied immune cells. Also, additional experiments using non-immune cells showed no effects of the signals on cytokine gene expression. We therefore conclude that these LF EMF exposure conditions are not expected to significantly modulate innate immune signaling. Bioelectromagnetics ß 2011 Wiley-Liss, Inc.

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“…It is already known that isolated animal cells have specific sensitivity to the strength and frequency of fluctuation of the TVMF [4][5][6]. The TVMF regulatory effect proposed to be associated with the ion cyclotron resonance mechanism [5] whose effect on extracted myocardial cells has been recently demonstrated under laboratory conditions [7]. Scientists have shown that healthy people in isolation from the magnetic field (MF) significantly increases their capillary blood flow and reduces the diastolic blood pressure [8].…”

Section: Introductionmentioning

confidence: 99%

The local earth magnetic field changes impact on weekly hospitalization due to unstable angina pectoris

Žiubrytė¹,

Jaruševičius²,

Landauskas³

et al. 2018

J., complex., health sci.

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Abstract.Objectives: Acute coronary syndrome as an acute oxygenated blood deprivation to the heart muscle due to atherosclerotic plaque rupture in the coronary artery followed by thrombosis is possibly associated with changes in the Earth's local time varying magnetic field as they strongly influence hormonal and other regulatory systems' activity. This study analyses the correlation between prevalence of the acute coronary syndrome and the changes in the local time varying aspects of the magnetic field. Methods: Seven-hundred patients admitted to Cardiology Department of Hospital of Lithuanian University of Health Sciences Kaunas Clinics within 2016 due to acute coronary syndrome were retrospectively included into the study. The number of cases per week was compared with the weekly changes of the local Earth magnetic field. The one-year period was divided into two periods: the first-half of the year (weeks 1 to 26) and the second-half of the year (weeks 27 to 52) and more detailed analyses were performed accordingly to the significance of the left main artery lesion. Mean power of local magnetic field fluctuations in Lithuania, measured in pT2 in five different frequency ranges where overlaps between the Schumann resonance and EEG frequency ranges (named as SDelta (0-3.5 Hz), STheta (3.5-7 Hz), SAlpha (7-15 Hz), SBeta (15-32 Hz) and SGamma (32-66 Hz) to distinguish them from the EEG bands). Results: Statistically significant weak and moderate correlations between weekly prevalence of acute coronary syndrome admissions and the magnetic field intensities changes were found. Higher intensities in the SBeta and SGamma ranges were associated with a higher number of admissions throughout the year in females and the SGamma range was associated with higher number of admission only during the second-half of the year in males. A higher intensity magnetic field in SDelta, STheta, SAlpha and SBeta ranges was associated with a higher admissions number due to left main artery lesions in males, while a higher intensity in the SGamma range was associated with higher number of admissions due to left main artery lesions in females through the year. Conclusion: Significant correlation between acute coronary syndrome and changes in the local Earth time varying magnetic field intensities was found. Some frequency ranges are associated with an episode of an acute coronary syndrome. Left main artery lesions significantly correlated with magnetic field changes in most of the frequency ranges in males while only one of the frequency ranges in females.

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Differentiation of human adult cardiac stem cells exposed to extremely low-frequency electromagnetic fields

Abstract: These results suggest that ELF-EMFs tuned at Ca2+-ICR could be used to drive cardiac-specific differentiation in adult cardiac progenitor cells without any pharmacological or genetic manipulation of the cells that will be used for therapeutic purposes.

Cited by 104 publications

References 33 publications

Effects of Fifty-Hertz Electromagnetic Fields on Granulocytic Differentiation of ATRA-Treated Acute Promyelocytic Leukemia NB4 Cells

Effects of Fifty-Hertz Electromagnetic Fields on Granulocytic Differentiation of ATRA-Treated Acute Promyelocytic Leukemia NB4 Cells

Low‐frequency electromagnetic fields do not alter responses of inflammatory genes and proteins in human monocytes and immune cell lines

The local earth magnetic field changes impact on weekly hospitalization due to unstable angina pectoris

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