Differentiation of glycans in equine guttural pouches

Parillo, Francesco; Rossi, Giacomo; Busoni, Valéria; Magi, Gian Enrico; Supplizi, Andrea Verini

doi:10.1016/j.tvjl.2007.12.024

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…Deacetylation (saponification) was performed by incubating sections with 1% potassium hydroxide solution in 70% ethanol for 15 minutes at room temperature prior to sialidase degradation. By detaching acetyl substituents, this chemical treatment renders sialic acid residues with O-acyl groups at C4 of the pyrannose ring that previously resisted C. perfringens neuraminidase susceptible to sialidase digestion (Parillo et al 2009d). …”

Section: Enzymatic Digestionmentioning

confidence: 99%

Lectin-binding sites in the seminal vesicles of entire and castrated horses

Parillo

Betti

Stacchiotti

et al. 2013

Journal of Applied Animal Research

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This research was undertaken to determine the glycoconjugates secreted by the glandular epithelium of the seminal vesicles in the entire and castrated horses using lectin histochemistry in combination with sialidase digestion and deglycosylation pre-treatments. The following lectins were used: Con-A, UEA-I, LTA, WGA, GSA-IB4, SBA, PNA, ECA and DBA. In the entire stallion, the glandular cells expressed the following sugar residues: a-Fuc, internal GlcNAc, terminal b-and a-GalNAc, b-D-Gal-(1Á4)-GlcNAc and a-Gal included in Olinked oligosaccharides; b-Gal-(1Á3)-GalNAc belonged to both O-and N-oligosaccharides whereas terminal GlcNAc belonged to N-linked glycans. Additionally, a-Gal and b-Gal acted as acceptor sugars for sialic acid moieties. In castrated horses, the glandular epithelium showed a different lectin labelling pattern. In particular, we evidenced internal GlcNAc, a-GalNAc, b-Gal-(1Á3)-GalNAc in both O-and N-linked glycoproteins whereas b-GalNAc and b-Gal-(1Á3)-GalNAc in O-linked glycoproteins. The differences evidenced in the lectin profile between the stallion and castrated horse suggested a hormonal regulation of the glycoconjugate production. Additionally, the plurality of glycomolecules detected in the secretions of the stallion may be involved in spermatozoa maturation.

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Section: Enzymatic Digestionmentioning

confidence: 99%