Differentiation of epithelial cells on microporous membranes

Patrone, Laura M.; Cook, Jacob; Crute, Barbara E.; Buskirk, Robert G. Van

doi:10.1007/bf01409015

Cited by 8 publications

(3 citation statements)

References 23 publications

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“…Since the porous nature of the bovine corneal endothelialdeposited membrane ruled out its use, we modified the experiments of Valentich (1982), who found that a basal lamina was deposited by MDCK cells when they were cultured on a supporting matrix of collagen fibrils. MDCK cells were subsequently shown to synthesize normal components of an epithelial basal lamina: type IV collagen, laminin, and heparan sulfate proteoglycan (perlecan) (Taub 1991;Patrone et al 1992).…”

Section: Discussionmentioning

confidence: 99%

A preformed basal lamina alters the metabolism and distribution of hyaluronan in epidermal keratinocyte "organotypic" cultures grown on collagen matrices

Tammi

Hascall

et al. 2000

Histochem Cell Biol

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A rat epidermal keratinocyte (REK) line which exhibits histodifferentiation nearly identical to the native epidermis when cultured at an air-liquid interface was used to study the metabolism of hyaluronan, the major intercellular macromolecule present in basal and spinous cell layers. Two different support matrices were used: reconstituted collagen fibrils with and without a covering basal lamina previously deposited by canine kidney cells. REKs formed a stratified squamous, keratinized epithelium on both support matrices. Hyaluronan and its receptor, CD44, colocalized in the basal and spinous layers similar to their distribution in the native epidermis. Most (approximately 75%) of the hyaluronan was retained in the epithelium when a basal lamina was present while most (approximately 80%) diffused out of the epithelium in its absence. While REKs on the two matrices synthesized hyaluronan at essentially the same rate, catabolism of this macromolecule was much higher in the epithelium on the basal lamina (half-life approximately 1 day, similar to its half-life in native human epidermis). The formation of a true epidermal compartment in culture bounded by the cornified layer on the surface and the basal lamina subjacent to the basal cells provides a good model within which to study epidermal metabolism.

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Section: Discussionmentioning

confidence: 99%

A preformed basal lamina alters the metabolism and distribution of hyaluronan in epidermal keratinocyte "organotypic" cultures grown on collagen matrices

Tammi

Hascall

et al. 2000

Histochem Cell Biol

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“…It also allows the use of various cell support materials, satisfying the specific requirements of different cells . A considerable influence of different support materials on the morphology and functionality has been documented for cultured epithelial (Patrone et al 1992) or endothelial cells (Villars et al 1996). To study the mammalian oviduct with regard to secretory activity, influence of hormones and growth factors and to investigate mechanisms of embryo-maternal communication, a cell culture system is needed resembling the oviduct epithelium in vivo most closely.…”

Section: Introductionmentioning

confidence: 99%

Factors affecting proliferation and dedifferentiation of primary bovine oviduct epithelial cells in vitro

Reischl

Prelle

Schöl

et al. 1999

Cell and Tissue Research

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The oviduct is the physiological site for key events in reproduction, such as capacitation of spermatozoa, fertilization and early embryonic development. Interactions between oviduct epithelial cells and gametes or embryos cannot sufficiently be studied in vivo. Therefore, model systems are needed which mimic in vivo conditions most closely. In this study we optimised the method for isolating bovine oviduct cells and compared different cell support materials as well as two culture systems (perfusion vs static culture) for their ability to maintain characteristic morphological and functional features of oviduct cells. Out of nine different cell support materials tested, cellulose nitrate (0.45 micron pore size) was the most suitable to maintain cells in a manner similar to freshly isolated oviduct epithelial cells. Comparing static vs perfusion culture by electron microscopy, morphological differences of the cells were insignificant in the first days of culture, while they became more evident after 8 days. The cells in the static system lost typical characteristics such as columnar shape, cilia and secretory protrusions, while these features were still present in perfusion culture. In addition, intense ciliogenesis and cytoplasmic organelles for protein synthesis were found under perfusion conditions. These findings were underlined by differences in expression of the oviduct-specific oestrus-associated glycoprotein 85-97 kDa (GP 85-97) gene as revealed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The RNA levels of this specific gene were significantly higher in perfusion compared to the static culture system. Our data show clear advantages of perfusion vs static culture for primary bovine oviduct epithelial cells.

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“…In general, epithelial cells form basement membranes for survival in cell culture systems [27] , [28] , [29] , [30] . However, detailed studies regarding the protein composition of basement membrane are lacking.…”

Section: Structure Of the Jementioning

confidence: 99%

Histological and immunological characteristics of the junctional epithelium

Nakamura

2018

Japanese Dental Science Review

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SummaryThe continuity of epithelial tissue is collapsed by tooth eruption. The junctional epithelium (JE) is attached to the tooth surface by hemidesmosomes, which constitutes the front-line defense against periodontal bacterial infection. JE constitutively expresses intercellular adhesion molecule-1 (ICAM-1), and neutrophils and lymphocytes penetrate into JE via interaction between ICAM-1 and LFA-1 expressed on the surface of these migrating cells. JE also expresses cytokines and chemokines. These functions of JE are maintained even in germ-free condition. Therefore, the constitutive expression of adhesion molecules, cytokines, and chemokines might be used not only for anti-pathogenic defense but also for maintaining the physiological homeostasis of JE. In this review, we have mainly focused on the structural and functional features of JE, and discussed the function of intraepithelial lymphocytes in JE as a front-line anti-microbial defense barrier and regulator of JE hemostasis.

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Differentiation of epithelial cells on microporous membranes

Cited by 8 publications

References 23 publications

A preformed basal lamina alters the metabolism and distribution of hyaluronan in epidermal keratinocyte "organotypic" cultures grown on collagen matrices

A preformed basal lamina alters the metabolism and distribution of hyaluronan in epidermal keratinocyte "organotypic" cultures grown on collagen matrices

Factors affecting proliferation and dedifferentiation of primary bovine oviduct epithelial cells in vitro

Histological and immunological characteristics of the junctional epithelium

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