Differentiation of embryonic stem cells into retinal neurons

Zhao, Xinshu; Liu, Jianuo; Ahmad, Iqbal

doi:10.1016/s0006-291x(02)02126-5

Cited by 105 publications

(77 citation statements)

References 27 publications

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“…The protocol to differentiate this cell type uses low concentrations of RA [45]. Crx, a transcription factor present in photoreceptor progenitor development, does not change with increasing RA or Pur concentration and is downregulated compared with controls not receiving RA or Pur.…”

Section: Discussionmentioning

confidence: 99%

Generation of V2a Interneurons from Mouse Embryonic Stem Cells

Brown

Butts

McCreedy

et al. 2014

Stem Cells and Development

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Section: Discussionmentioning

confidence: 99%

Generation of V2a Interneurons from Mouse Embryonic Stem Cells

Brown

Butts

McCreedy

et al. 2014

Stem Cells and Development

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“…10 Zhao and coworkers also reported that retinal progenitor cells could induce ES cell differentiation into retinal neurons when ES cells were co-cultured with retinal progenitor cells. 23 This observation suggests that the mature extracellular environment presents signals that induce early stage stem cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Alternative culture conditions for isolation and expansion of retinal progenitor cells

Qiu

Seiler

Arai

et al. 2004

Current Eye Research

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Purpose. To investigate different in vitro model systems for retinal progenitor cell (RPC) isolation and expansion.Methods. RPCs were isolated from embryonic day (E) 17 Long Evans rat retinas. Three different culture media: (1) modified serum free defined media (2) serum-containing media and (3) embryonic stem cell (ES)-conditioned media were used for RPC isolation and long term expansion Expression of various cellular markers and cell morphologies were compared among the three culture systems at different passages by immunostaining and confocal microscopy.Results. All three culture systems could maintain RPCs as nestin-positive cells (78-87%) after long-term in vitro expansion. However, RPCs appeared to proliferate faster in the serum-free culture system. The ES-conditioned media provided the best RPC survival. Cells appeared smaller at early passages compared with later passages. This morphology change occurred at P9-P10 in the serum-free medium, and at P5-P6 in the other two culture systems.Conclusions. The serum-free medium may be superior for preventing RPC differentiation during expansion.

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“…In addition to primary RSCs derived from retinal tissue, RSCs can be derived from embryonic stem cells (ESCs) [29,30]. Embryonic stem cells can be expanded extensively to generate the large numbers of cells needed for commercial development.…”

Section: Rscsmentioning

confidence: 99%

Stem Cells for Retinal Replacement Therapy

Stern

Temple

2011

Neurotherapeutics

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Retinal degenerative disease has limited therapeutic options and the possibility of stem cell-mediated regenerative treatments is being actively explored for these blinding retinal conditions. The relative accessibility of this central nervous system tissue and the ability to visually monitor changes after transplantation make the retina and adjacent retinal pigment epithelium prime targets for pioneering stem cell therapeutics. Prior work conducted for several decades indicated the promise of cell transplantation for retinal disease, and new strategies that combine these established surgical approaches with stem cell-derived donor cells is ongoing. A variety of tissue-specific and pluripotent-derived donor cells are being advanced to replace lost or damaged retinal cells and/or to slow the disease processes by providing neuroprotective factors, with the ultimate aim of long-term improvement in visual function. Clinical trials are in the early stages, and data on safety and efficacy are widely anticipated. Positive outcomes from these stem cell-based clinical studies would radically change the way that blinding disorders are approached in the clinic.

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Differentiation of embryonic stem cells into retinal neurons

Cited by 105 publications

References 27 publications

Generation of V2a Interneurons from Mouse Embryonic Stem Cells

Generation of V2a Interneurons from Mouse Embryonic Stem Cells

Alternative culture conditions for isolation and expansion of retinal progenitor cells

Stem Cells for Retinal Replacement Therapy

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