Differentiation of drug and non-drug Cannabis using a single nucleotide polymorphism (SNP) assay

Rotherham, D.; Harbison, SallyAnn

doi:10.1016/j.forsciint.2010.10.006

Cited by 53 publications

(69 citation statements)

References 31 publications

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“…Liquid chromatography methods developed more recently can detect both acidic and neutral cannabinoids, therefore providing a more precise characterisation of chemotype (De Backer et al 2009). Several DNA markers associated with the genes encoding THCA and/or CBDA synthase have been found beneficial in predicting chemotype during early stages of plant development (Kojoma et al 2006;Pacifico et al 2006;Rotherham and Harbison 2011;Staginnus et al 2014), with the most comprehensively studied in terms of genetic linkage and sample population screening being the dominant D589 (Staginnus et al 2014) and co-dominant B1080/B1192 (Pacifico et al 2006) DNA sequence characterised amplified region (SCAR) markers respectively.…”

Section: Introductionmentioning

confidence: 99%

“…To date, the available chemotyping and genotyping methods have only been applied to a subset of the C. sativa gene pool (Pacifico et al 2006;Rotherham and Harbison 2011;Staginnus et al 2014). Given the extensive genetic (Faeti et al 1996;Gao et al 2014;Gilmore et al 2007;Hillig 2005) and chemotypic variability (Baker et al 1980;Hillig and Mahlberg 2004) which appears to exist, use of each approach in isolation may not be sufficient to account for the full extent of variation in cannabinoid composition within the species.…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of cannabinoid composition in a diverse Cannabis sativa L. germplasm collection

et al. 2015

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The ability to characterise cannabinoid chemical phenotype (chemotype) accurately is important for the development of Cannabis sativa L. cultivars specific for pharmacological, hemp fibre, or seed end use. Although a number of chemotyping and genotyping methods have previously been developed to predict and characterise cannabinoid composition, only a subset of the gene pool has been examined. A representative survey from a wide range of geographically and genetically diverse C. sativa accessions using liquid chromatography-mass spectrometry (LC-MS) cannabinoid profiling together with dominant and co-dominant DNA marker assays was performed. Overall variability of chemotype across the gene pool was found to be three-fold greater within heterozygote genotypes than previously reported. Interestingly, an individual plant of East Asian origin was found to exhibit a rare propyl alkyl cannabinoid homologue and a chemotype inconsistent with the predicted genotype. We propose that in order to carry out comprehensive screening of genetic resource collections and to identify chemotypic variants specific for end-use pharmacological applications, a strategy which adopts both cannabinoid profiling and the co-dominant DNA marker assay is required. Further research with consideration of propyl-alkyl-cannabinoid homologues should explore the relationship between chemotype and genotype in greater detail.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterisation of cannabinoid composition in a diverse Cannabis sativa L. germplasm collection

et al. 2015

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“…25) It has been suggested that 37 amino acid substitutions corresponding to the polymorphism determine the ability of THCA synthesis. [25][26][27] THCA synthase gene does not have high homology with any genes of other species. In this study, we have developed a novel LAMP assay for detecting C. sativa by targeting the conserved region of THCA synthase gene.…”

Section: 7)mentioning

confidence: 98%

Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of <i>Cannabis sativa</i>

Kitamura

Aragane

Nakamura

et al. 2016

Biological & Pharmaceutical Bulletin

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In many parts of the world, the possession and cultivation of Cannabis sativa L. are restricted by law. As chemical or morphological analyses cannot identify the plant in some cases, a simple yet accurate DNA-based method for identifying C. sativa is desired. We have developed a loop-mediated isothermal amplification (LAMP) assay for the rapid identification of C. sativa. By optimizing the conditions for the LAMP reaction that targets a highly conserved region of tetrahydrocannabinolic acid (THCA) synthase gene, C. sativa was identified within 50 min at 60-66°C. The detection limit was the same as or higher than that of conventional PCR. The LAMP assay detected all 21 specimens of C. sativa, showing high specificity. Using a simple protocol, the identification of C. sativa could be accomplished within 90 min from sample treatment to detection without use of special equipment. A rapid, sensitive, highly specific, and convenient method for detecting and identifying C. sativa has been developed and is applicable to forensic investigations and industrial quality control.

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“…DNA polymorphisms in the THCA synthase gene can be used to discriminate "drug-type" and "fiber-type" Cannabis (Kojoma et al, 2006;Rotherham and Harbison, 2011). DNA polymorphisms in the THCA synthase gene can be used to discriminate "drug-type" and "fiber-type" Cannabis (Kojoma et al, 2006;Rotherham and Harbison, 2011).…”

Section: Chemotaxonomymentioning

confidence: 99%

Phytochemical and biological research of Cannabis pharmaceutical resources

Hao¹,

Gu²,

Xiao³

2015

Medicinal Plants

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Differentiation of drug and non-drug Cannabis using a single nucleotide polymorphism (SNP) assay

Cited by 53 publications

References 31 publications

Characterisation of cannabinoid composition in a diverse Cannabis sativa L. germplasm collection

Characterisation of cannabinoid composition in a diverse Cannabis sativa L. germplasm collection

Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of <i>Cannabis sativa</i>

Phytochemical and biological research of Cannabis pharmaceutical resources

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