Differentiation of Campylobacter species by AFLP fingerprinting

Duim, Birgitta; Vandamme, Peter; Rigter, Alan; Laevens, Severine; Dijkstra, Jeroen R.; Wagenaar, Jaap A.

doi:10.1099/00221287-147-10-2729

Cited by 72 publications

(49 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PCR-RFLP analysis of the 23S rRNA gene with two restriction enzymes is able to discriminate between Campylobacter species, but interpretation of the results is complicated by intervening sequences (11). More recently, amplified fragment length polymorphism fingerprinting has proven to be useful for Campylobacter species identification (4,27), but the method is laborious and expensive. Whole-genome DNA-DNA hybridization analysis allows species identification, but the method is not suitable for routine use (34).…”

mentioning

confidence: 99%

Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences

2004

View full text Add to dashboard Cite

The phylogeny of 12 Campylobacter species and reference strains of Arcobacter butzleri and Helicobacter pylori was studied based on partial 593-bp groEL gene sequences. The topology of the phylogenetic neighbor-joining tree based on the groEL gene was similar to that of the tree based on the 16S rRNA gene. However, groEL was found to provide a better resolution for Campylobacter species, with lower interspecies sequence similarities (range, 65 to 94%) compared with those for the 16S rRNA gene (range, 90 to 99%) and high intraspecies sequence similarities (range, 95 to 100%; average, 99%). A new universal reverse primer that amplifies a 517-bp fragment of the groEL gene was developed and used for PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of 68 strains representing 11 Campylobacter species as well as reference strains of A. butzlerii and H. pylori. Digestion with the AluI enzyme discriminated all Campylobacter species included in the study but showed more intraspecies diversity than digestion with the ApoI enzyme. A hippurate-negative variant of Campylobacter jejuni with a high level of groEL sequence similarity to both C. jejuni (96%) and C. coli (94%) gave a unique AluI profile and an ApoI profile identical to those of other C. jejuni strains. In conclusion, groEL gene sequencing and PCR-RFLP analysis are recommended as valuable tools for the identification of Campylobacter species.

show abstract

mentioning

confidence: 99%

Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences

2004

View full text Add to dashboard Cite

show abstract

“…However, mutation of the hipO gene has been noticed such as deletion or mutations in the primer binding sites, resulting in no PCR amplification (31) or a PCR product shorter than expected (9). Hence, a number of rapid and accurate identification methods based on the genetic traits targeting species-specific genes have been developed for Campylobacter species (1,6,11,14,15,19,20,34). These methods typically require the use of hybridization probes, multiple restriction enzymes or sophisticated equipment, which are beyond the means of many diagnostic laboratories.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Cytolethal Distending Toxin (cdt) Gene‐Based Species‐Specific Multiplex PCR Assay for the Identification of Campylobacter Strains Isolated from Poultry in Thailand

Samosornsuk

Asakura

Yoshida

et al. 2007

Microbiology and Immunology

View full text Add to dashboard Cite

We have recently developed a cytolethal distending toxin (cdt) gene‐based species‐specific multiplex PCR assay for identifying Campylobacter jejuni, C. coli and C. fetus. In the present study, the applicability of this assay was evaluated with 34 Campylobacter‐like organisms isolated from poultry in Thailand for species identification and was compared with other assays including API Campy, 16S rRNA gene sequence, and hippuricase (hipO) gene detection. Of the 34 strains analyzed, 20, 10 and 1 were identified as C. jejuni, C. coli, and Arcobacter cryaerophilus, respectively, and 3 could not be identified by API Campy. However, 16S rRNA gene analysis, showed that all 34 strains are C. jejuni/coli. To discriminate between these 2 species, the hipO gene, which is specifically present in C. jejuni, was examined by PCR and was detected in 20 strains, which were identified as C. jejuni by API Campy but not in the remaining 14 strains. Collective results indicated that 20 strains were C. jejuni whereas the 14 strains were C. coli. When the cdt gene‐based multiplex PCR was employed, however, 19, 20 and 19 strains were identified as C. jejuni while 13, 14 and 13 were identified as C. coli by the cdtA, cdtB and cdtC gene‐based multiplex PCR, respectively. Pulsed‐field gel electrophoresis revealed that C. jejuni and C. coli strains analyzed are genetically diverse. Taken together, these data suggest that the cdt gene‐based multiplex PCR, particularly cdtB gene‐based multiplex PCR, is a simple, rapid and reliable method for identifying the species of Campylobacter strains.

show abstract

“…In the present study, we continued to investigate (6,10,12,21,25,29,35) the genetic differences within the species C. fetus, including isolates that were C. fetus subsp. fetus or C. fetus subsp.…”

Section: Discussionmentioning

confidence: 99%

Genetic Divergence of Campylobacter fetus Strains of Mammal and Reptile Origins

Tu¹,

Eisner

Kreiswirth

2005

J Clin Microbiol

View full text Add to dashboard Cite

Campylobacter fetus is a gram-negative bacterial pathogen of both humans and animals. Two subspecies have been identified, Campylobacter fetus subsp. fetus and Campylobacter fetus subsp. venerealis, and there are two serotypes, A and B. To further investigate the genetic diversity among C. fetus strains of different origins, subspecies, and serotypes, we performed multiple genetic analyses by utilizing random amplification of polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE), and DNA-DNA hybridization. All 10 primers used for the RAPD analyses can distinguish C. fetus strains of reptile and mammal origin, five can differentiate between C. fetus subsp. fetus and C. fetus subsp. venerealis strains, and four showed differences between type A and type B isolates from mammals. PFGE with SmaI and SalI digestion showed varied genome patterns among different C. fetus strains, but for mammalian C. fetus isolates, genome size was well conserved (mean, 1.52 ؎ 0.06 Mb for SmaI and 1.52 ؎ 0.05 Mb for SalI). DNA-DNA hybridization demonstrated substantial genomic-homology differences between strains of mammal and reptile origin. In total, these data suggest that C. fetus subsp fetus strains of reptile and mammal origin have genetic divergence more extensive than that between the two subspecies and that between the type A and type B strains. Combining these studies with sequence data, we conclude that there has been substantial genetic divergence between Campylobacter fetus of reptile and mammal origin. Diagnostic tools have been developed to differentiate among C. fetus isolates for taxonomic and epidemiologic uses.

show abstract

Differentiation of Campylobacter species by AFLP fingerprinting

Cited by 72 publications

References 27 publications

Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences

Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences

Evaluation of a Cytolethal Distending Toxin (cdt) Gene‐Based Species‐Specific Multiplex PCR Assay for the Identification of Campylobacter Strains Isolated from Poultry in Thailand

Genetic Divergence of Campylobacter fetus Strains of Mammal and Reptile Origins

Contact Info

Product

Resources

About