Differentiation of Bean pod mottle virus isolates based upon host symptoms

Abstract

Accessions from Glycine, Phaseolus, and Vigna genera were screened for their reactions to different subgroups of isolates of Bean pod mottle virus (BPMV) in order to establish a differential host system. Screening results indicated that the BPMV isolates differed in pathogenic aggressiveness but not in virulence. No major resistance genes were found in soybean (Glycine max) or G. soja since all screened accessions showed mosaic or necrotic symptoms to BPMV inoculation. However, these accessions expressed differences in severity of symptoms when challenged by various BPMV isolates. The inoculation of G. tomentella accessions did not result in mosaic symptoms, and some accessions did not support systemic infection of some of the isolates. Resistance, presented as a hypersensitive reaction, was observed in some of Phaseolus and Vigna genotypes, and resistant response or susceptibility was stable to all the isolates used in the screening. In conclusion, the selected G. soja genotypes PI 407019, PI 464889A, and PI 464928, and 'Amsoy 71' soybean may help to separate severe (reassortant) from mild isolates of BPMV based upon their phenotypic reactions.

Reactions of Mid‐Southern U.S. Soybean Cultivars to Bean Pod Mottle Virus and Tobacco Ringspot Virus

“…1). A similar diff erence between these two isolates was also observed in a previous study by Mozzoni et al (2008).…”

“…Therefore, the tested cultivars were divided into four groups (Table 1). A similar diff erence between these two isolates was also observed in a previous study by Mozzoni et al (2008). The second group includes those cultivars that showed 26 to 50% biomass reduction in infected plants.…”

“…Bean pod mottle virus can be transmitted mechanically, by certain insects, and through infected seeds (Zaumeyer and Thomas, 1948;Pitre, 1970;Patel and Pitre, 1971;Giesler et al, 2002;Werner et al, 2003). In addition, a hypersensitive reaction associated with host plant defense system was observed in some of Phaseolus and Vigna genotypes (Mozzoni et al, 2008). To date, no BPMV resistance genes have been found in G. max (Zheng et al, 2005), although it is known that soybean reactions to BPMV range from mild chlorotic mottling to severe mosaic (Ross, 1968;Walters, 1970;Giesler et al, 2002).…”

“…Bean pod mottle virus symptoms appear mainly on leaves ( Fig. 1) and include mosaic (ranging from mild to severe), chlorosis, and leaf rugosity, all of which can contribute to yield reduction (Ross, 1963;Walters, 1970;Windham and Ross, 1985;Giesler et al, 2002;Mozzoni et al, 2008). The virus also infects plant stems, causing them to remain green even after pod maturity (referred to as "green stem"), reducing canopy and plant height in soybean ( Fig.…”

“…However, resistance and tolerance to BPMV in a few accessions of Glycine tomentella Hayata and G. max were reported, respectively (Windham and Ross, 1985;Hill et al, 2007;Zheng et al, 2005). In addition, a hypersensitive reaction associated with host plant defense system was observed in some of Phaseolus and Vigna genotypes (Mozzoni et al, 2008).…”