Differentiation of Adult Stem Cells into Smooth Muscle for Vascular Tissue Engineering

Harris, Lisa J.; Abdollahi, Hamid; Zhang, Ping; McIlhenny, Stephen; Tulenko, Thomas N.; DiMuzio, Paul

doi:10.1016/j.jss.2009.08.001

Cited by 95 publications

(73 citation statements)

References 24 publications

(40 reference statements)

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“…These markers are intermediate to late markers of smooth muscle cell differentiation, unlike a-smooth muscle actin, which is considered to be an early marker of smooth muscle differentiation and, importantly, is not limited only to expression in smooth muscle cells. 35 The mRNA and protein expression for these contractile apparatus markers was significantly higher for cells attached to the microcarriers and incubated in TGFb-1 compared with the microcarriers incubated in the control medium when quantified by QPCR and in-cell Western, indicating that TGFb-1 enhanced the differentiation process. The expression of myosin heavy chain was only significantly expressed in cells incubated in the differentiation medium for 14 days ( p < 0.01).…”

Section: Figmentioning

confidence: 89%

“…A similar approach has previously been used to induce differentiation of MSCs into smooth muscle-like cells when cultured on 2D tissue culture surfaces. 34,35 Differentiation following TGF-b1 binding results in signaling through the Smad pathway, causing inhibition of cell FIG. 3.…”

Section: Discussionmentioning

confidence: 99%

“…Color images available online at www.liebertpub.com/tec proliferation and induction of contractile proteins in smooth muscle cells. [34][35][36] Incubation of the TIPS microcarriers in the differentiation medium resulted in the induced expression of the three myogenic markers-caldesmon, calponin, and myosin heavy chains. These markers are intermediate to late markers of smooth muscle cell differentiation, unlike a-smooth muscle actin, which is considered to be an early marker of smooth muscle differentiation and, importantly, is not limited only to expression in smooth muscle cells.…”

Section: Figmentioning

confidence: 99%

See 2 more Smart Citations

A Novel Method for Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle-Like Cells on Clinically Deliverable Thermally Induced Phase Separation Microspheres

Parmar

Ahmadi²,

Day³

2015

Tissue Engineering Part C: Methods

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Muscle degeneration is a prevalent disease, particularly in aging societies where it has a huge impact on quality of life and incurs colossal health costs. Suitable donor sources of smooth muscle cells are limited and minimally invasive therapeutic approaches are sought that will augment muscle volume by delivering cells to damaged or degenerated areas of muscle. For the first time, we report the use of highly porous microcarriers produced using thermally induced phase separation (TIPS) to expand and differentiate adipose-derived mesenchymal stem cells (AdMSCs) into smooth muscle-like cells in a format that requires minimal manipulation before clinical delivery. AdMSCs readily attached to the surface of TIPS microcarriers and proliferated while maintained in suspension culture for 12 days. Switching the incubation medium to a differentiation medium containing 2 ng/ mL transforming growth factor beta-1 resulted in a significant increase in both the mRNA and protein expression of cell contractile apparatus components caldesmon, calponin, and myosin heavy chains, indicative of a smooth muscle cell-like phenotype. Growth of smooth muscle cells on the surface of the microcarriers caused no change to the integrity of the polymer microspheres making them suitable for a cell-delivery vehicle. Our results indicate that TIPS microspheres provide an ideal substrate for the expansion and differentiation of AdMSCs into smooth muscle-like cells as well as a microcarrier delivery vehicle for the attached cells ready for therapeutic applications.

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Section: Figmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

See 1 more Smart Citation

A Novel Method for Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle-Like Cells on Clinically Deliverable Thermally Induced Phase Separation Microspheres

Parmar

Ahmadi²,

Day³

2015

Tissue Engineering Part C: Methods

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“…Among the differentially expressed proteins in IVM or IVO pES cells, caldesmon 1 was highly expressed in IVM pES cells compared with that in fES cells. Caldesmon is a calmodulin-binding protein that inhibits ATPase activity of myosin in smooth muscle (Huber, 1998) and is upregulated during differentiation of adult stem cells into smooth muscle (Harris et al, 2011). Prdx4, FBP1, the nuclear matrix protein senescence evasion factor (SNEV), and stomatin-like protein 2 were upregulated in IVO pES cells.…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomics analysis of parthenogenetically induced pluripotent stem cells

Wang

Xie

et al. 2011

Protein Cell

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Parthenogenetic embryonic stem (pES) cells isolated from parthenogenetic activation of oocytes and embryos, also called parthenogenetically induced pluripotent stem cells, exhibit pluripotency evidenced by both in vitro and in vivo differentiation potential. Differential proteomic analysis was performed using differential in-gel electrophoresis and isotope-coded affinity tag-based quantitative proteomics to investigate the molecular mechanisms underlying the developmental pluripotency of pES cells and to compare the protein expression of pES cells generated from either the in vivo-matured ovulated (IVO) oocytes or from the in vitro-matured (IVM) oocytes with that of fertilized embryonic stem (fES) cells derived from fertilized embryos. A total of 76 proteins were upregulated and 16 proteins were downregulated in the IVM pES cells, whereas 91 proteins were upregulated and 9 were downregulated in the IVO pES cells based on a minimal 1.5-fold change as the cutoff value. No distinct pathways were found in the differentially expressed proteins except for those involved in metabolism and physiological processes. Notably, no differences were found in the protein expression of imprinted genes between the pES and fES cells, suggesting that genomic imprinting can be corrected in the pES cells at least at the early passages. The germline competent IVM pES cells may be applicable for germ cell renewal in aging ovaries if oocytes are retrieved at a younger age.

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“…These proteins were found to be associated with various stages of SMCs differentiation. SM-MHC and CALP are expressed predominantly in the mature SMCs whereas CK-8 expression is typical for fetal and poorly differentiated cells [8,9].…”

Section: Introductionmentioning

confidence: 99%

Potentially positive ageing-related variations of medial smooth muscle cells in the saphenous veins used as aortocoronary bypass grafts

Perek

Malińska

Gąsowski

et al. 2015

Folia Histochem Cytobiol.

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Introduction. Currently, elderly people constitute a large proportion of patients undergoing coronary artery bypass grafting (CABG). Activated smooth muscle cells in the tunica media of saphenous vein (SV) grafts are thought to play a key role in the formation of neointima and development of occluding atherosclerotic plaques. The aim of this study was to identify ageing-related variations in the expression of the smooth muscle cells proteins that may impact on patency rate of the grafts and the CABG outcomes. Material and methods. The study involved 216 consecutive patients with the mean of 62.7 ± 8.4 years who underwent isolated CABG with at least one SV aortocoronary bypass graft. Expression of a-smooth muscle actin (a-SM actin), smooth muscle-myosin heavy chain (SM-MHC), calponin (CALP), cytokeratin 8 (CK-8), metalloproteinase-2 (MMP-2) and tissue inhibitors of metalloproteinases-2 and -3 (TIMP-2, TIMP-3) in the SV wall was assessed by immunohistochemistry and correlated with the age of patients. Results. Calponin and a-SM actin were expressed in all studied SV transplants. SM-MHC immunoreactivity was observed in SV segments in 68.5% of patients, whereas MMP-2a and TIMPs expression was found in 75% of cases. In more than 50% of analyzed SV transplants, no expression of cytokeratin-8 was found. Moderate correlations between preexisting expressions of either cytoskeletal or hemostatic proteins in the tunica media of the SV grafts and the age of CABG patients were demonstrated. They were positive for SM-MHC (r = 0.494), CALP (r = 0.548), TIMP-2 (r = 0.413) and TIMP-3 (r = 0.406) whereas negative for CK-8 (r = -0.528) and MMP-2 (r = -0.417). Conclusions. Age-dependent decreases in the expression of MMP-2 and CK-8 accompanied by increases in expression of SM-MHC, TIMP-2 and TIMP-3 may promote SV graft patency and, thus, suggest a rationale for common use of SV grafts in the elderly.

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Differentiation of Adult Stem Cells into Smooth Muscle for Vascular Tissue Engineering

Abstract: Background-Herein we evaluate the potential of adipose-derived stem cells (ASC) to differentiate into smooth muscle cells (SMC) and their potential for use in a tissue-engineered vascular graft.

Cited by 95 publications

References 24 publications

A Novel Method for Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle-Like Cells on Clinically Deliverable Thermally Induced Phase Separation Microspheres

A Novel Method for Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle-Like Cells on Clinically Deliverable Thermally Induced Phase Separation Microspheres

Quantitative proteomics analysis of parthenogenetically induced pluripotent stem cells

Potentially positive ageing-related variations of medial smooth muscle cells in the saphenous veins used as aortocoronary bypass grafts

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