Differentiation “in vitro” of primary and immortalized porcine mesenchymal stem cells into cardiomyocytes for cell transplantation

Moscoso, Isabel; Centeno, Alberto; López, E. J. Díaz; Rodríguez-Barbosa, José-Ignacio; Santamarina, Isabel; Filgueira, Pedro González; Sánchez, María‐José; Domínguez‐Perles, Raúl; Peñuelas-Rivas, G.; Doménech, Nieves

doi:10.1016/j.transproceed.2004.12.247

Cited by 73 publications

(49 citation statements)

References 6 publications

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“…Cells were injected directly in proximity or within the injured myocardium, acutely or chronically after the ischemic event, and the effects of these interventions were determined several weeks later. In all cases, myocardial regeneration was documented and the reconstitution of dead myocardium correlated with the improvement in ventricular function and reappearance of wall motion activity (321,424). Moreover, cotransplantation of human MSCs and human fetal cardiomyocytes resulted in an amelioration of cardiac performance greater than with MSCs alone (311).…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 88%

Cardiac Stem Cells and Mechanisms of Myocardial Regeneration

Leri

Kajstura²,

Anversa³

2005

Physiological Reviews

396

349

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This review discusses current understanding of the role that endogenous and exogenous progenitor cells may have in the treatment of the diseased heart. In the last several years, a major effort has been made in an attempt to identify immature cells capable of differentiating into cell lineages different from the organ of origin to be employed for the regeneration of the damaged heart. Embryonic stem cells (ESCs) and bone marrow-derived cells (BMCs) have been extensively studied and characterized, and dramatic advances have been made in the clinical application of BMCs in heart failure of ischemic and nonischemic origin. However, a controversy exists concerning the ability of BMCs to acquire cardiac cell lineages and reconstitute the myocardium lost after infarction. The recognition that the adult heart possesses a stem cell compartment that can regenerate myocytes and coronary vessels has raised the unique possibility to rebuild dead myocardium after infarction, to repopulate the hypertrophic decompensated heart with new better functioning myocytes and vascular structures, and, perhaps, to reverse ventricular dilation and wall thinning. Cardiac stem cells may become the most important cell for cardiac repair.

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Section: Mesenchymal Stem Cellsmentioning

confidence: 88%

Cardiac Stem Cells and Mechanisms of Myocardial Regeneration

Leri

Kajstura²,

Anversa³

2005

Physiological Reviews

396

349

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“…Prior to the selection of MP2, a review of the published literature showed a high diversity in the reagents used, and for this reason we decided to adapt a new medium (with 5-azacytidine) [Xu et al, 2004;Moscoso et al, 2005;Antonitsis et al, 2007;Zhang et al, 2009;Meligy et al, 2012]. During the first phase, epigenetic changes in the cell are the key to muscle differentiation with changes in chromatin remodeling that allow access to myogenic regulatory factors (MRFs) to start myogenesis [Lodish, 2004].…”

Section: Discussionmentioning

confidence: 99%

PPAR Agonists Promote the Differentiation of Porcine Bone Marrow Mesenchymal Stem Cells into the Adipogenic and Myogenic Lineages

Pérez-Serrano

González-Dávalos²,

Lozano-Flores³

et al. 2016

Cells Tissues Organs

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Purpose: The aim of this work was to evaluate the effect of PPAR agonists on the differentiation and metabolic features of porcine mesenchymal stem cells induced to the adipogenic or myogenic lineages. Methods: Bone marrow MSCs from neonate pigs were isolated and identified by cell proliferation, cell surface markers or the gene expression of stem cells (CD44, CD90, CD105 or Oct4 and Nanog, respectively). Cells were differentiated into adipose or muscle cells and treated with the PPAR agonists; adipogenic and myogenic differentiation was promoted by adding these compounds. The expression of PPARγ (an adipose marker) and MyoD1 and MyHC (muscle markers), metabolic changes and expression levels of metabolic enzymes involved in glycolysis, lipogenesis, lipolysis and the pentose phosphate pathway were tested by qPCR. Results: MSCs from neonate pigs exhibited high proliferation and were positive for CD44, CD90 and CD105 markers and Oct4 and Nanog expression. The treatment that promoted the highest expression of PPARγ was 50 µM of conjugated linoleic acid (CLA) c9 t11 (6.44 ± 0.69-fold, p ≤ 0.0001) in the adipose differentiation, and upregulation of HX2, ACCAα, ATGL, LPL and G6DP (p ≤ 0.0001) and downregulation of PFK and ACCAβ (p ≤ 0.0001) were found. For muscle differentiation, the best treatment was 50 µM of CLA c10 t12 (59.72 ± 4.72-fold, p ≤ 0.0001), and metabolic changes were upregulation of PFK, ACCAβ, G6DP, CPT1 and PPARβ/δ (p ≤ 0.0001), but no effect was observed with HX2 and ACCAα (p ≥ 0.05). Conclusions: Our results suggest that differentiated cells exhibit a typical cell lineage metabolism and higher efficiencies both in anabolism and catabolism.

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“…First reports pointed to the DNA methyltransferase inhibitor -5-azacitidine as the factor initializing myogenesis of MSCs [82]. Some studies confirm, that the addition of 5-azacitidine to the culture medium induces the expression of myogenic cells marker -desmin [75] or cardiomyocytes markers -β-myosin heavy chain and cardiac troponin-T [76,83] in harvested MSCs. On the other hand, there are reports questioning such an effect of 5-azacitidine.…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 97%

Myogenic stem cells.

Burdzińska

Gala²,

Pączek³

2009

Folia Histochem Cytobiol.

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Both skeletal muscle and bone marrow tissue contain myogenic stem cells. The population residing in muscles is heterogenic. Predominant in number are "typical" satellite cells -muscle progenitors migrating from somites during embryonic life. Another population is group of multipotent muscle stem cells which, at least in part, are derived from bone marrow. These cells are tracked by gradient of growth factors releasing from muscle during injury or exercise. Recruited bone marrow-derived cells gradually change their phenotype becoming muscle stem cells and eventually can attain satellite cell position and express Pax7 protein. Mesenchymal stem cells (MSC) isolated directly from bone marrow also display myogenic potential, although methods of induction of myogenic differentiation in vitro have not been optimized yet. Concerning efforts of exploiting myogenic stem cells in cell-mediated therapies it is important to understand the cause of impaired regenerative potential of aged muscle. Up to now, most of research data suggest that majority of age related changes in skeletal muscles are reversible, thus depending on extrinsic factors. However, irreversible intrinsic features of muscle stem cells are also taken into consideration.

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Differentiation “in vitro” of primary and immortalized porcine mesenchymal stem cells into cardiomyocytes for cell transplantation

Cited by 73 publications

References 6 publications

Cardiac Stem Cells and Mechanisms of Myocardial Regeneration

Cardiac Stem Cells and Mechanisms of Myocardial Regeneration

PPAR Agonists Promote the Differentiation of Porcine Bone Marrow Mesenchymal Stem Cells into the Adipogenic and Myogenic Lineages

Myogenic stem cells.

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