Differentiation in the status of self-incompatibility among Calibrachoa species (Solanaceae)

Tsukamoto, Tatsuya; Ando, Toshio; Watanabe, Hitoshi; Kokubun, Hisashi; Hashimoto, Goro; Sakazaki, Ushio; Suarez, Enrique; Marchesi, Eduardo; Oyama, Ken; Kao, Teh Hui

doi:10.1007/s102650200024

Cited by 20 publications

(28 citation statements)

References 12 publications

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“…We have summarized the morphological classes and SI/ SC status of all populations of P. axillaris examined here, and those from previous studies Tsukamoto et al 1998; Table 5). Ando et al (1998) classified all 28 Uruguayan populations of subsp.…”

Section: Subsp Parodii and Subsp Subandinamentioning

confidence: 99%

“…In accordance with the methods used in previous studies Tsukamoto et al 1998), at least ten flower buds of each individual were kept closed by use of a staple 1 day before anthesis to prevent cross-pollination. At the mature flower stage on the following day the closed flowers were flicked with a finger to enable self-pollination.…”

Section: Status Of Self-(in)compatibilitymentioning

confidence: 99%

“…parodii is SC (Table 5). Tsukamoto et al (1998) classified nine populations of P. axillaris subsp. subandina from Argentina as SC (strong SC, according to our criteria) on the basis of a similar self-pollination experiment.…”

Section: Subsp Parodii and Subsp Subandinamentioning

confidence: 99%

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Distribution of self-compatible and self-incompatible populations of Petunia axillaris (Solanaceae) outside Uruguay

et al. 2006

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Petunia axillaris occurs in temperate South America and consists of three allopatric subspecies: axillaris, parodii, and subandina. Previous studies have revealed that subsp. axillaris is self-incompatible (SI), subsp. parodii is self-compatible (SC) in Uruguay, and subsp. subandina is SC in Argentina. The SI/SC status over the entire distribution range is not completely understood, however. The objective of this study was to examine the overall SI/SC status of the respective subspecies in comparison with floral morphology. The results confirmed that subsp. parodii and subsp. subandina were SC throughout the distribution range, and that subsp. axillaris was also SC in Brazil and in most of the Argentinean territory. The SI P. axillaris occurs in the natural population only between 34 and 36 degrees S, along the eastern shore of South America. The Brazilian and Uruguayan subsp. axillaris differed in SI/SC status and floral morphology. We discuss the cause of this difference.

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Section: Subsp Parodii and Subsp Subandinamentioning

confidence: 99%

Section: Status Of Self-(in)compatibilitymentioning

confidence: 99%

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Distribution of self-compatible and self-incompatible populations of Petunia axillaris (Solanaceae) outside Uruguay

et al. 2006

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“…We have examined previously the SI status of all 19 natural taxa of the genus Petunia (Solanaceae; Tsukamoto et al, 1998), including three allopatric infraspecific taxa of the species Petunia axillaris (Lam.) Britton, Sterns & Poggenb., a historical parent of garden petunias (Sink, 1984).…”

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confidence: 99%

Breakdown of Self-Incompatibility in a Natural Population ofPetunia axillaris Caused by Loss of Pollen Function

et al. 2003

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Although Petunia axillaris subsp. axillaris is described as a self-incompatible taxon, some of the natural populations we have identified in Uruguay are composed of both self-incompatible and self-compatible plants. Here, we studied the selfincompatibility (SI) behavior of 50 plants derived from such a mixed population, designated U83, and examined the cause of the breakdown of SI. Thirteen plants were found to be self-incompatible, and the other 37 were found to be selfcompatible. A total of 14 S-haplotypes were represented in these 50 plants, including two that we had previously identified from another mixed population, designated U1. All the 37 self-compatible plants carried either an S C1 -or an S C2 -haplotype. S C1 S C1 and S C2 S C2 homozygotes were generated by self-pollination of two of the self-compatible plants, and they were reciprocally crossed with 40 self-incompatible S-homozygotes (S 1 S 1 through S 40 S 40 ) generated from plants identified from three mixed populations, including U83. The S C1 S C1 homozygote was reciprocally compatible with all the genotypes examined. The S C2 S C2 homozygote accepted pollen from all but the S 17 S 17 homozygote (identified from the U1 population), but the S 17 S 17 homozygote accepted pollen from the S C2 S C2 homozygote. cDNAs encoding S C2 -and S 17 -RNases were cloned and sequenced, and their nucleotide sequences were completely identical. Analysis of bud-selfed progeny of heterozygotes carrying S C1 or S C2 showed that the SI behavior of S C1 and S C2 was identical to that of S C1 and S C2 homozygotes, respectively. All these results taken together suggested that the S C2 -haplotype was a mutant form of the S 17 -haplotype, with the defect lying in the pollen function. The possible nature of the mutation is discussed.Naturally occurring or induced (e.g. by radiation) self-compatible mutants of self-incompatible species in the Solanaceae have provided useful materials for studying the mechanism of the S-RNase-mediated self-incompatibility (SI) system. For example, molecular genetic studies of a self-compatible line of Lycopersicon peruvianum have suggested that the RNase activity of S-RNases is an integral part of the SI mechanism because mutations in the S-RNase gene rendering its protein product without the RNase activity cause the breakdown of SI (Kowyama et al., 1994a;Royo et al., 1994). Moreover, cytological and molecular genetic studies of x-ray irradiationinduced self-compatible mutants have uncovered a phenomenon, called competitive interaction, where duplication of the S-locus region renders pollen grains carrying two different pollen S-alleles unable to function in SI (de Nettancourt, 1977;Golz et al., 2000). Studies of self-compatible mutants have also led to the identification of loci unlinked to the S-locus that are required for the function of the S-RNase gene or the yet unidentified pollen S-gene. For example, mutations in the HT gene, which encodes a small Asn-rich protein (McClure et al., 1999), are partially responsible for the breakdown o...

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“…However, since the corolla of INT used as the pollen parent of P. × hybrida was found to display reddish purple color (Paxton 1836), it is safe to conclude that the progenitor harbored a functional F3′5′H gene. Although it cannot be ruled out that the progenitor originally exhibited the hf1-2 heterozygous genotype because INT and INF are self-incompatible (Tsukamoto et al 1998) and outcrossing species, it is likely that the insertion of dTph9 occurred after the first cross was made between AXI (or PRD) and INT (or INF).…”

Section: Origin Of Hf1-2 and Hf1-3 Allelesmentioning

confidence: 99%

Reconstructing Historical Events that Occurred in the Petunia Hf1 Gene, which Governs Anthocyanin Biosynthesis, and Effects of Artificial Selection by Breeding

et al. 2007

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The nucleotide sequence of a gene, Hf1, encoding the flavonoid 3′,5′-hydroxylase in commercial petunias was compared to those in natural species to reconstruct historical events that occurred at the Hf1 locus in commercial petunias in the course of breeding since the 1830s. Intact Hf1 genes of any natural species so far studied were not detectable in 84 commercial petunias examined and were considered to have been removed by breeders. The dominant Hf1 alleles isolated from 14 Hf1 Hf1 cultivars showed an identical open reading frame (ORF) sequence and were considered to result from inter-allelic recombination between Hf1 of Petunia axillaris subsp. axillaris or subsp. parodii and Hf1 of P. integrifolia subsp. integrifolia or P. inflata, which were recorded as or assumed to be the historical parents of commercial petunias. Recessive hf1-3 was considered to be a mutant allele of the recombined Hf1 associated with the insertion of a retrotransposable element (rTph1) into exon III. The recessive hf1-2 allele showed a sequence identical to that of Hf1 of P. integrifolia subsp. integrifolia and P. inflata, except for a transposable element (dTph9) inserted into exon III and a target site duplication. We analyzed the potential causes of the recombination that occurred at the Hf1 locus and of the removal of dominant Hf1 genes of natural species from commercial petunias.

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Differentiation in the status of self-incompatibility among Calibrachoa species (Solanaceae)

Cited by 20 publications

References 12 publications

Distribution of self-compatible and self-incompatible populations of Petunia axillaris (Solanaceae) outside Uruguay

Distribution of self-compatible and self-incompatible populations of Petunia axillaris (Solanaceae) outside Uruguay

Breakdown of Self-Incompatibility in a Natural Population ofPetunia axillaris Caused by Loss of Pollen Function

Reconstructing Historical Events that Occurred in the Petunia Hf1 Gene, which Governs Anthocyanin Biosynthesis, and Effects of Artificial Selection by Breeding

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