Differentiation in cultures derived from embryonic chicken muscle

Turner, David C.; Gmür, Rudolf; Lebherz, Herbert G.; Siegrist, Marianne; Wallimann, Theo; Eppenberger, Hans M.

doi:10.1016/0012-1606(76)90091-9

Cited by 48 publications

(4 citation statements)

References 40 publications

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“…Similarly, it was found that the appea'rance of AChR and CPK in fetal calf muscle,cultures are coupled and furthermore that their elaboration did not depend on cell fusion [10]. The recent reports [6][7][8]11,12], that cytoplasmic proteins accumulated in fusion-arrested chicken myoblasts, further support the conclusion that cell fusion is not a prerequisite forPthese manifestations of skeletal muscle differentiation. On the other hand, close association between cell fusion and the synthesis of muscle specific proteins in rat cultures was claimed [ 19 ].…”

Section: Discussionsupporting

confidence: 55%

“…The correlation between cell fusion and the appearance of muscle-specific proteins has led some authors to hypothesize that the fusion process might be a prerequisite for the protein formation [1][2][3]. Recently, however, some authors have suggested that myogenesis, as manifested by the appearance of muscle proteins, is independent of the cell fusion process [4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The appearance of acetylcholine receptors triggered by fusion of myoblasts in vitro

Shainberg

Brik

1978

FEBS Letters

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Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

The appearance of acetylcholine receptors triggered by fusion of myoblasts in vitro

Shainberg

Brik

1978

FEBS Letters

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“…The same sequence of reagents gave positive cytoplasmic staining in muscle cell cultures (for method, see ref. 19), demonstrating that the labeled antibody used was capable of reacting with Fab' fragments (not shown). Experiments with control Fab' showed that the failure to stain could not be attributed to destruction of antigen by residual papain activity in the Fab' preparations: There was no specific binding of labeled antibody after incubation with control Fab' (not shown) but, when myofibrils were allowed to react after having been incubated first with control Fab' and then with ant-iMM-CK IgG, normal M-line staining was observed ( Fig.…”

mentioning

confidence: 99%

Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils.

Wallimann¹,

Pelloni²,

Turner³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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Column-purified antibodies against creatine kinase (EC 2.7.3.2) from chicken skeletal muscle (the homodimeric isoenzyme designated MM-CK) bind specifically to the M lines of chicken pectoral muscle myofibrils. Incubation of myofibrils with monovalent Fab' fragments of these antibodies solubilizes most of the myofibril-bound creatine kinase, concomitantly removing most of the electron-dense material from the M lines. This strongly indicates that MM-CK is an integral part of the M-line structure and is consistent with the suggestion that MM-CK molecules form the M bridges that are responsible for the principle M-line substriations.The M line in vertebrate striated muscle runs transversely through the middle of the A band. Electron microscopy has revealed considerable structural complexity in this part of each sarcomere. Cross sections through the M line show a network of transverse elements, called (primary) M bridges (1,2). Each of the three (or five, depending on fiber type) principal substriations seen in longitudinal sections corresponds to an array of M bridges (1-3,f). M bridges apparently do not interconnect thick filaments directly but instead link each filament to six longitudinal elements called M filaments (1, t). Each M filament is apparently joined to four other M filaments by secondary M bridges, and there is evidence for still other transverse elements.t The protein composition of these different M-line elements is unknown.

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“…Confluence was typically reached after 7-9 d in culture. Primary cultures of pectoral muscle myoblasts (41) and pectoral muscle and dorsal skin fibroblasts were prepared by Dr . M. Sieber-Blum from 10-d-old White Leghorn chick embryos (Truslow Farm, Chestertown, Md .)…”

Section: Cellsmentioning

confidence: 99%

Quantitative analysis of intercellular adhesive specificity in freshly explanted and cultured cells.

Sieber¹,

Roseman²

1981

The Journal of Cell Biology

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A new method is presented for the quantitative analysis of intercellular adhesive specificity . In this assay, two cell types are mixed, one unlabeled and the other labeled with the fluorescent dye, fluorescamine [4-phenylspiro(feran-2[3H],1'-phthalan)-3,3'-dione] . The resulting aggregates are analyzed by fluorescence microscopy to determine the number of labeled and unlabeled cells per aggregate. Random (nonspecific) aggregation was characterized by a binomial distribution, and adhesive specificity was accordingly quantified by the deviation (as determined by a chi-square test) from the calculated binomial distribution . The labeling procedure was simple and rapid, and experiments with 18 different cell types showed that it did not affect cell viability, morphology, rate and extent of adhesion, plating efficiency, and the capability of myogenic cells to undergo terminal differentiation . Most important, assays with morphologically identifiable cell pairs indicated that the fluorescent label neither induced apparent nor destroyed existing adhesive specificity .The most pronounced adhesive specificities were observed with freshly explanted cells from adult tissues and also with mixtures of simian virus 40-transformed and nontransformed BALB/c 3T3 cells . A glucosamine-6-phosphate N-acetylase-deficient mutant 3T3 line (AD6), however, aggregated randomly with parental 3T3 cells . Lectin-resistant mutant Chinese hamster ovary (CHO) cells displayed marginal adhesive specificity when mixed with normal CHO cells.

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Differentiation in cultures derived from embryonic chicken muscle

Cited by 48 publications

References 40 publications

The appearance of acetylcholine receptors triggered by fusion of myoblasts in vitro

The appearance of acetylcholine receptors triggered by fusion of myoblasts in vitro

Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils.

Quantitative analysis of intercellular adhesive specificity in freshly explanted and cultured cells.

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