Differentiation dependent expression and distinct subcellular localization of the protooncogene product, PEBP2β/CBFβ, in muscle development

Chiba, Natsuko; Watanabe, Toshio; Nomura, Shosaku; Tanaka, Yuta; Minowa, Muneo; Niki, Masaru; Kanamaru, Ryunosuke; Satake, Masanobu

doi:10.1038/sj.onc.1201109

Cited by 26 publications

(28 citation statements)

References 21 publications

(29 reference statements)

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“…One of the interesting aspects of the cytoplasmic PEBP2b/CBFb is our immunohistochemical observation that the protein was colocalized with the a-actinin on the Z-lines or their vicinity in the established skeletal muscle ®bers (Chiba et al, 1997). This observation suggests an a nity of PEBP2b/CBFb with cytoskeletal structures.…”

Section: Introductionmentioning

confidence: 58%

“…The amounts of proteins from each fraction which corresponded to the equivalent cell number were separated by SDSpolyacrylamide gel electrophoresis (PAGE) and blotted onto a membrane. The ®lter was probed with anti-b peptide antibody (Figure 1) whose preparation and speci®city were described previously (Chiba et al, 1997). As seen in lane 4, a signi®cant proportion of the 22 kilodaltons PEBP2b/CBFb protein which were expressed endogenously in the cells was found to be retained in the cytoskeleton fraction.…”

Section: Resultsmentioning

confidence: 99%

“…The anti-PEBP2b/CBFb peptide antibody was raised in hens as described previously (Chiba et al, 1997). This antibody was puri®ed for immunocytochemical staining by a nity chromatography on a column in which the peptide was linked to cyanogen bromide-activated Sepharose 4B (Pharmacia).…”

Section: The Antibodymentioning

confidence: 99%

“…The proteins were separated by electrophoresis on a 0.1% (wt/vol) SDS ± 12% (wt/vol) polyacrylamide gel. The transfer of the proteins from the gel onto the ®lter, the blocking reaction, incubation of the ®lter with the appropriate antibodies and visualization of the immunocomplexes using ABC kit and ECL detection reagents were as described previously (Chiba et al, 1997).…”

Section: Western Blot (Immunoblot) Analysismentioning

confidence: 99%

“…Only when the b was coexpressed with a truncated form of the a protein, did the b subunit become detectable in the nucleus. Secondly, we recently observed by immunohistochemistry that the PEBP2b/CBFb was expressed in a di erentiation dependent manner in the skeletal myogenic cells of murine embryos, and that the protein mainly accumulated in the cytoplasm (Chiba et al, 1997). Only during a limited stage of di erentiation, namely in the multinucleated myotubes, was nuclear staining observed for PEBP2b/CBFb.…”

Section: Introductionmentioning

confidence: 98%

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The protooncogene product, PEBP2β/CBFβ, is mainly located in the cytoplasm and has an affinity with cytoskeletal structures

et al. 1997

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Section: Introductionmentioning

confidence: 58%

Section: Resultsmentioning

confidence: 99%

Section: The Antibodymentioning

confidence: 99%

Section: Western Blot (Immunoblot) Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

The protooncogene product, PEBP2β/CBFβ, is mainly located in the cytoplasm and has an affinity with cytoskeletal structures

et al. 1997

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Function of CBFβ/Bro proteins

Adya

Castilla

Liu

2000

Seminars in Cell & Developmental Biology

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Transcriptional networks regulating the costamere, sarcomere, and other cytoskeletal structures in striated muscle

Estrella

Naya

2013

Cell. Mol. Life Sci.

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Structural abnormalities in striated muscle have been observed in numerous transcription factor gain- and loss-of-function phenotypes in animal and cell culture model systems, indicating that transcription is important in regulating the cytoarchitecture. While most characterized cytoarchitectural defects are largely indistinguishable by histological and ultrastructural criteria, analysis of dysregulated gene expression in each mutant phenotype has yielded valuable information regarding specific structural gene programs that may be uniquely controlled by each of these transcription factors. Linking the formation and maintenance of each subcellular structure or subset of proteins within a cytoskeletal compartment to an overlapping but distinct transcription factor cohort may enable striated muscle to control cytoarchitectural function in an efficient and specific manner. Here we summarize available evidence that connects transcription factors, those with established roles in striated muscle such as MEF2 and SRF as well as other non-muscle transcription factors, to the regulation of a defined cytoskeletal structure. The notion that genes encoding proteins localized to the same subcellular compartment are coordinately transcriptionally regulated may prompt rationally designed approaches that target specific transcription factor pathways to correct structural defects in muscle disease.

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Differentiation dependent expression and distinct subcellular localization of the protooncogene product, PEBP2β/CBFβ, in muscle development

Cited by 26 publications

References 21 publications

The protooncogene product, PEBP2β/CBFβ, is mainly located in the cytoplasm and has an affinity with cytoskeletal structures

The protooncogene product, PEBP2β/CBFβ, is mainly located in the cytoplasm and has an affinity with cytoskeletal structures

Function of CBFβ/Bro proteins

Transcriptional networks regulating the costamere, sarcomere, and other cytoskeletal structures in striated muscle

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