Differentiation‐dependent enhanced expression of protein phosphatase 2Cβ in germ cells of mouse seminiferous tubules

Disruption of the mouse protein Ser/Thr phosphatase 2Cβ gene leads to early pre-implantation lethality

“…1A, a-d). This is consistent with our previous observation that the mRNA levels of three isoforms of PP2Cb (b-3, -4, and -5) increased over the course of mouse spermatogenesis, especially after the pachytene phase (Kato et al, 1996). In MII oocytes from wild-type mice, PP2Cb was localized to the spindle complex and cytoplasm (Fig.…”

“…Conversely, the mRNA level of PP2Ca, a PP2C isoform that shares the highest overall similarity (about 75%) with PP2Cb of all family members, is very low in MII oocytes and zygotes but sharply increases thereafter until the four-cell stage Hamatani et al, 2004). We have previously shown that the levels of PP2Cb-3, -4, and -5 mRNA increase specifically in germ cells in the testis during the first wave of spermatogenesis, especially after the pachytene stage, and that the PP2Cb protein level is much higher in the testes of adult mice compared with newborns (Kato et al, 1996). Collectively, these observations raise the possibility that PP2Cb plays a unique role in gametogenesis, fertilization, and/or the development of pre-implantation embryos.…”

A novel protein phosphatase 2C family member (PP2Cζ) is able to associate with ubiquitin conjugating enzyme 91

“…These results suggest that the expression of PP2Cζ is developmentally regulated in the testicular germ cells. It has been established that the first wave of spermatogenesis takes place in a synchronized manner in mouse testis during the first 30‐day period after birth [25]. Therefore, we determined by Northern blot analysis whether the expression level of PP2Cζ altered during the course of this first wave of spermatogenesis.…”

“…4A ). However, no such band was observed in an artificial mouse cryptorchid testis, which had type A spermatogonia but no germ cells in more advanced stages of differentiation, or testes of the mutant mice (jsd/jsd, SI 17H /SI 17H and W/W v ) which were defective of spermatogenesis [18,23–25]. We then studied the cell type distribution of PP2Cζ mRNA using germ cell, Leydig cell and Sertoli cell fractions.…”

Protein phosphatase 2Cα expression in normal human tissues: an immunohistochemical study

“…The enzyme was found in bovine retina (Klumpp et al 1998), in rat, monkey, and human livers (Kikuchi et al 1997;Ortmeyer 1997;Saadat et al 1994), in mouse testicular germ cells (Hou et al 1994;Kato et al 1996), in rat parotid acinar cells (Yokoyama et al 1995(Yokoyama et al , 1996, in bovine and rat brains (Abe et al 1992;Wang et al 1995), in rat adipocytes (Wood et al 1993), and in human cardiac and skeletal muscles (Marley et al 1998). The enzyme was found in bovine retina (Klumpp et al 1998), in rat, monkey, and human livers (Kikuchi et al 1997;Ortmeyer 1997;Saadat et al 1994), in mouse testicular germ cells (Hou et al 1994;Kato et al 1996), in rat parotid acinar cells (Yokoyama et al 1995(Yokoyama et al , 1996, in bovine and rat brains (Abe et al 1992;Wang et al 1995), in rat adipocytes (Wood et al 1993), and in human cardiac and skeletal muscles (Marley et al 1998).…”