Differentiation, chemical profiles and quality evaluation of five medicinal Stephania species (Menispermaceae) through integrated DNA barcoding, HPLC-QTOF-MS/MS and UHPLC-DAD

Zhao, Wanli; Liu, Manyu; Shen, Chen; Liu, Hanqing; Zhang, Zhentang; Dai, Wenling; Liu, Xiufeng; Liu, Jihua

doi:10.1016/j.fitote.2019.104453

Cited by 16 publications

(8 citation statements)

References 33 publications

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“…Several HPLC fingerprints have been reported for identification in the quality control of Saussurea involucrata in which samples for study were from different batches and areas [ 11 – 14 ]. These samples were not identified with DNA barcoding, and therefore they may not cover a total of four haplotypes of Saussurea involucrata in our previous study based on ITS2 sequences, and its four haplotypes are consistent with the results of gene sequences recorded in GenBank and the DNA Barcoding System for Identifying Herbal Medicine, since a medicinal plant has multiple haplotypes on the basis of variable sites when being tested by DNA barcoding, and HPLC fingerprinting has been used for assessment of chemical variability in herbal medicine [ 15 , 16 ]. The chemical profile of herbal drugs was mostly affected by the medicinal plant gene.…”

Section: Introductionsupporting

confidence: 59%

Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA

Chen

Zhang

et al. 2020

Journal of Analytical Methods in Chemistry

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The quality control of Saussurea involucrata has been greatly improved by macroscopic and microscopic identification and chemical profiling described in Chinese Pharmacopoeia since 2005. However, these methods have their own limitations, e.g., their dependence on personal experience and expertise, and it is a huge challenge to identify closely related species that share similar or identical morphological characteristics and chemical profiles. A novel and generally accepted identification strategy is urgently needed as a complement to regulations for protecting the public health interests. In this work, a comprehensive chromatographic fingerprint method was developed and tested on 43 samples from four haplotypes of S. involucrata according to DNA barcoding. Three common patterns consisting of 20, 14, and 7 common peaks were generated by frequency filters of median, upper quartile, and 100%, respectively. Based on two formerly screened patterns, S. involucrata can be effectively identified from its five easily confused snow lotus species, including the most closely related plant (S. orgaadayi) in the orthogonal partial least-squares discriminant analysis (OPLS-DA) models. The model is supported by good R and Q coefficients. In addition, different haplotypes of S. involucrata can be discriminated in the OPLS-DA model using the 20 common peaks. Among them, peaks 9, 11, 16 (zaluzanin C), and 18 (dehydrocostus lactone) have been identified as fingerprint markers of S. involucrata via S-plots and VIP values (>1). Additionally, peaks 19 and 20 were identified as linolenic acid and linoleic acid with anti-inflammatory activity, and they were isolated from the herb for the first time. Collectively, the chromatographic fingerprint of S. involucrata can be an effective and integrated method for the identification of authentic herbs from adulterant species or related plants, and discrimination of its different haplotypes provides an objective and reliable tool for quality control.

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Section: Introductionsupporting

confidence: 59%

Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA

Chen

Zhang

et al. 2020

Journal of Analytical Methods in Chemistry

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“…Stephania epigaea H. S. Lo, 1978 (Menispermaceae) is distributed mainly in Yunnan and Sichuan provinces, China (Zhao et al. 2020 ; Dong et al. 2015 ), frequently used to treat cough, diarrhea, bellyache, injuries, and malaria (Xiao et al.…”

mentioning

confidence: 99%

The complete chloroplast genome sequence of the medicinal plant Stephania epigaea H. S. Lo, 1978 (Menispermaceae) from Yunnan, China

Guan

Feng

Fan

2022

Mitochondrial DNA Part B

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Stephania epigaea H. S. Lo, 1978 is a medicinal plant commonly used in southwest China. This study characterized the first complete chloroplast (cp) genome sequence of this species. The complete cp was 157,738 bp in length, containing a large single-copy region (LSC) of 88,460 bp, a small single-copy region (SSC) of 19,778 bp, and a pair of inverted repeat regions (IRs) of 24,750 bp. It encoded 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The GC content of the complete genome was 36.7%. Phylogenetic analysis of complete cp sequences revealed that S. epigaea was clustered with S. japonica from the Menispermaceae family.

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“…Previously mentioned that the medicinal plant Stephania epigaea Lo traditionally was used to treat fever, cough, malaria, diarrhea, bellyache, injuries from falls, and fracture [ 3 , 4 ] and newly discovered activities of antiproliferative/anticancer, immunomodulating, and apoptosis [ 7 , 38 ]. Our metabolomic analysis shows that 518 metabolites were detected from the root, stem, and flowers of S. epigaea through widely targeted metabolomics analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Stephania epigaea Lo, belonging to the family Menispermaceae, is an herbaceous liana that primarily grows mainly in limestone hills and is found in Guangdong, Guangxi, Hainan, Yunnan, and Sichuan provinces of China, where it is called “dì bù róng,” “jīn bù huàn,” or “shān wū guī” [ 1 , 2 ]. Its root tuber has been used as a traditional folk medicine for anti-inflammatory, relieving pain, and sedation to treat cancer, fever, cough, malaria, diarrhea, bellyache, stomachache, and injuries from falls and fractures by local people [ 3 – 5 ]. A total of 40 alkaloids have been identified from the plant since the study of their chemical constituents was first reported in 1975 [ 6 ], which are divided into seven categories, including protoberberine-, aporphine-, morphine-, hasubanan-, benzylisoquinoline-, bisbenzylisoquinoline-, and azafluoranthene-type alkaloids, which have been evaluated for biological activity, such as acetylcholinesterase (AChE) inhibitory, cytotoxic, anti-inflammatory, antitumor activities [ 7 – 10 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Bioactive Components of Stephania epigaea Lo and Their Potential Therapeutic Targets by UPLC-MS/MS and Network Pharmacology

Mao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Stephania epigaea, an important traditional folk medicinal plant, elucidating its bioactive compound profiles and their molecular mechanisms of action on human health, would better understand its traditional therapies and guide their use in preclinical and clinical. This study aims to detect the critical therapeutic compounds, predict their targets, and explore potential therapeutic molecular mechanisms. This work first determined metabolites from roots, stems, and flowering twigs of S. epigaea by a widely targeted metabolomic analysis assay. Then, the drug likeness of the compounds and their pharmacokinetic profiles were screened by the ADMETlab server. The target proteins of active compounds were further analyzed by PPI combing with GO and KEGG cluster enrichment analysis. Finally, the interaction networks between essential compounds, targets, and disease-associated pathways were constructed, and the essential compounds binding to their possible target proteins were verified by molecular docking. Five key target proteins (EGFR, HSP90AA1, SRC, TNF, and CASP3) and twelve correlated metabolites, including aknadinine, cephakicine, homostephanoline, and N-methylliriodendronine associated with medical applications of S. epigaea, were identified, and the compounds and protein interactions were verified. The key active ingredients are mainly accumulated in the root, which indicates that the root is the main medicinal tissue. This study demonstrated that S. epigaea might exert the desired disease efficacy mainly through twelve components interacting via five essential target proteins. EGFR is the most critical one, which deserves further verification by biological studies.

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Differentiation, chemical profiles and quality evaluation of five medicinal Stephania species (Menispermaceae) through integrated DNA barcoding, HPLC-QTOF-MS/MS and UHPLC-DAD

Cited by 16 publications

References 33 publications

Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA

Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA

The complete chloroplast genome sequence of the medicinal plant Stephania epigaea H. S. Lo, 1978 (Menispermaceae) from Yunnan, China

Identification of Bioactive Components of Stephania epigaea Lo and Their Potential Therapeutic Targets by UPLC-MS/MS and Network Pharmacology

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