Differentiation capacity of stromal fibroblast-like cells from human bone marrow, adipose tissue, hair follicle dermal papilla and derma

Ev, Kiseleva; Chermnykh, E. S.; Vorotelyak, E. A.; Volozhin, A I; Vasiliev, A. V.; Terskikh, V. V.

doi:10.1134/s1990519x09010064

Cited by 6 publications

(11 citation statements)

References 33 publications

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“…Near 20% of cells were lipid-filled. These results were similar to what we obtained with DP cells isolated by the method described earlier (Wu et al 2005;Kiseleva et al 2009). …”

Section: Differentiation Potentialsupporting

confidence: 93%

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Dermal papilla cells induce keratinocyte tubulogenesis in culture

Chermnykh

Vorotelyak

Gnedeva

et al. 2010

Histochem Cell Biol

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The ability of dermal papilla (DP) cells to induce hair growth was reported in many studies. However, early stages of hair follicle development and signals that govern this process are poorly understood. Therefore, an in vitro model may be a convenient system to study epithelial-mesenchymal interactions and early stages of epidermal morphogenesis, especially in humans. To investigate the role of DP cells in epidermal morphogenesis we modified the method of isolation of DP cells from hair follicle of human scalp and developed the three-dimensional model of epidermal morphogenesis. Isolated DP cells were able to differentiate in adipogenic and osteogenic directions and retained activity of alkaline phosphatase (AP) for seven passages in culture. DP cells were able to induce tubule-like structures in three-dimensional model in vitro and to reorganize collagen matrix. Prolonged cultivation of DP cells has been a big problem because of the loss of hair follicle-inducing ability and growth activity after several passages. To solve this problem we immortalized DP cells by the transfection of the human telomerase reverse transcriptase cDNA (hTERT). Immortalized DP-hTERT cells retained AP activity and demonstrated low ability to osteogenic differentiation. The conditioned medium collected from actively proliferated cells as well as DP-hTERT cells themselves were capable to induce tubulogenesis after prolonged keratinocyte cultivation.

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“…Near 20% of cells were lipid-filled. These results were similar to what we obtained with DP cells isolated by the method described earlier (Wu et al 2005;Kiseleva et al 2009). …”

Section: Differentiation Potentialsupporting

confidence: 93%

“…1d). This pattern of staining corresponded to that of DPs on hair follicle sections shown before (Kiseleva et al 2009). …”

Section: Characteristics Of Dp Cell Culturesmentioning

confidence: 55%

Dermal papilla cells induce keratinocyte tubulogenesis in culture

Chermnykh

Vorotelyak

Gnedeva

et al. 2010

Histochem Cell Biol

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“…For the previous 20 years it has been found that the main property and chief biological role of DP cells are their ability to induce and regulate morphogenesis of a hair follicle [36]. However, findings of the investigation [37] demonstrated that DP are capable of differentiating in osteogenic and adipogenic direction, therefore, they may be referred to the classical mesenchymal fibroblast-like cells with special functions. It is the reference of DP cells to MSCs gives grounds to suggest that a skin equivalent containing DP cells will speed up neoangiogenesis, remodeling of extracellular matrix, formation of granulation tissue and skin regeneration.…”

Section: A Cellular Componentmentioning

confidence: 81%

Skin Tissue-Engineering Constructs and Stem Cells Application for the Skin Equivalents Creation (Review)

Meleshina¹,

Bystrova²,

Rogovaya³

et al. 2017

Sovrem Tehnol Med

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Development and introduction of new biotechnological analogues (equivalents) of tissues and organs into clinical practice, such as human skin equivalents (SE), designed for temporal or permanent replacement of damaged or destroyed tissue, remains an urgent problem of regenerative medicine. Currently, full-thickness SE as well as separate skin layers, which include living cells of different types, are being created and investigated. Since the ideal skin substitutes have not been created, the efforts of researchers in many countries are aimed at solving this problem.In our review, we present a comparative analysis of existing SE, both commercial and those being at the stage of preclinical study, analyze their structure and feasibility of application for solving experimental and clinical tasks. Characteristics of the three main variants of SE have also been considered. Examples of stem cell application for creation of skin equivalents have been given. The main advantages of using stem cells as a cell component of skin equivalents have been described.

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“…These characteristics are widely accepted criteria for mesenchymal stem cells including ibroblastic morphology, ability to adhere and to diferentiate into osteogenic, chondrogenic, and adipogenic lineages [24,27,28]. It should be mentioned that DP diferentiation abilities are often pronounced not to the same extent as those of classical mesenchymal stem cells [23]. As other lineages of diferentiation were demonstrated some authors believe DP cells to be multipotent [28].…”

Section: Dermal Papilla Cells and Their Inductive Propertiesmentioning

confidence: 99%

“…At the same time, DP cells meet stem cell criteria. In a number of works, including those coming from our lab, it was demonstrated that DP cells, according to their characteristics, may be atributed to mesenchymal stem cells along with those derived from bone marrow and adipose tissue [23][24][25][26]. These characteristics are widely accepted criteria for mesenchymal stem cells including ibroblastic morphology, ability to adhere and to diferentiate into osteogenic, chondrogenic, and adipogenic lineages [24,27,28].…”

Section: Dermal Papilla Cells and Their Inductive Propertiesmentioning

confidence: 99%

Hair Follicle Reconstruction and Stem Cells

Kalabusheva¹,

Chermnykh²,

Terskikh³

et al. 2017

Hair and Scalp Disorders

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De novo hair follicle (HF) formation in embryonic skin and hair growth in postnatal skin are the result of epithelial-mesenchymal interactions between specialized mesenchymal dermal papilla (DP) and epithelial stem cells that give rise to hairs. Adult HF is a valuable source of diferent lineages of stem cells (SCs) with morphogenetic potential. Epithelial stem cells are residing in the special compartment of HF (the bulge) and can be mobilized to regenerate the new follicle with each hair cycle and to reepithelialize epidermis during wound repair. This review summarizes the current knowledge on key characteristics of HF SC populations in terms of regenerative potential. General biological principles that govern the mesenchymal-epithelial interactions within the HF and the signaling pathways that control HF development are discussed. The main focus is on recent approaches to reconstruct folliculogenesis in vitro and perspectives of the tissue engineering in alopecia therapy.

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Differentiation capacity of stromal fibroblast-like cells from human bone marrow, adipose tissue, hair follicle dermal papilla and derma

Cited by 6 publications

References 33 publications

Dermal papilla cells induce keratinocyte tubulogenesis in culture

Dermal papilla cells induce keratinocyte tubulogenesis in culture

Skin Tissue-Engineering Constructs and Stem Cells Application for the Skin Equivalents Creation (Review)

Hair Follicle Reconstruction and Stem Cells

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