Differentiation between wild and artificial cultivated <i>Stephaniae tetrandrae</i> radix using chromatographic and flow‐injection mass spectrometric fingerprints with the aid of principal component analysis

Qin, Ya-Dong; Fang, Fengman; Wang, Rongbin; Zhou, Juanjuan; Li, Lin‐hua

doi:10.1002/fsn3.1717

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…Pharmacological studies have shown that the fungus contains biologically active chemical substances including nucleosides, cordycepic acid, cordycepin, beauvericin, and myriocin ( Sun et al, 2017 ). C. cicadae has been historically utilized for liver and kidney protection, analgesia–antipyresis, blood fat reduction, and its antitumor activities ( Qin et al, 2020 ). It is well known that the active ingredients extracted from C. cicadae are effective in ameliorating CKD induced by diabetes or hypertension.…”

Section: Introductionmentioning

confidence: 99%

Cordyceps cicadae Ameliorates Renal Hypertensive Injury and Fibrosis Through the Regulation of SIRT1-Mediated Autophagy

Cai

Feng²,

Jia

et al. 2022

Front. Pharmacol.

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Hypertensive renal injury is a complication of hypertension. Cordyceps cicadae (C. cicadae) is a traditional Chinese medicine used to treat chronic kidney diseases especially renal fibrosis. Autophagy is described as a cell self-renewal process that requires lysosomal degradation and is utilized for the maintenance of cellular energy homeostasis. The present study explores the mechanism underlying C. cicadae’s renoprotection on hypertensive nephropathy (HN). First, HN rat models were established on spontaneously hypertensive rats (SHRs). The expression of fibrosis-related protein and autophagy-associated protein was detected in vivo. NRK-52E cells exposed to AngII were chosen to observe the potential health benefits of C. cicadae on renal damage. The level of extracellular matrix accumulation was detected using capillary electrophoresis immunoquantification and immunohistochemistry. After treatment with lysosomal inhibitors (chloroquine) or an autophagy activator (rapamycin), the expression of Beclin-1, LC3II, and SQSTM1/p62 was further investigated. The study also investigated the change in sirtuin1 (SIRT1), fork head box O3a (FOXO3a), and peroxidation (superoxide dismutase (SOD) and malondialdehyde (MDA)) expression when intervened by resveratrol. The changes in SIRT1 and FOXO3a were measured in patients and the SHRs. Here, we observed that C. cicadae significantly decreased damage to renal tubular epithelial cells and TGFβ1, α-smooth muscle actin (α-SMA), collagen I (Col-1), and fibronectin expression. Meanwhile, autophagy defects were observed both in vivo and in vitro. C. cicadae intervention significantly downregulated Beclin-1 and LC3II and decreased SQSTM1/p62, showing an inhibition of autophagic vesicles and the alleviation of autophagy stress. These functions were suppressed by rapamycin, and the results were just as effective as the resveratrol treatment. HN patients and the SHRs exhibited decreased levels of SIRT1 and FOXO3a. We also observed a positive correlation between SIRT1/FOXO3a and antifibrotic effects. Similar to the resveratrol group, the expression of SIRT1/FOXO3a and oxidative stress were elevated by C. cicadae in vivo. Taken together, our findings show that C. cicadae ameliorates tubulointerstitial fibrosis and delays HN progression. Renoprotection was likely attributable to the regulation of autophagic stress mediated by the SIRT1 pathway and achieved by regulating FOXO3a and oxidative stress.

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Section: Introductionmentioning

confidence: 99%

Cordyceps cicadae Ameliorates Renal Hypertensive Injury and Fibrosis Through the Regulation of SIRT1-Mediated Autophagy

Cai

Feng²,

Jia

et al. 2022

Front. Pharmacol.

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“…The PCA method is used for feature extraction and dimension reduction; it converts numerous variables into a small number of relevant variables, allowing a clearer understanding of categories, clusters, and outliers [27]. In PCA, the model is visualized, allowing it to be understandable and avoiding subjective judgment [28].…”

Section: Discussionmentioning

confidence: 99%

Explorative Study on Volatile Organic Compounds of Cinnamon Based on GC-IMS

Pan,

Qiao,

Liu

et al. 2024

Metabolites

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Cinnamon is one of the most popular spices worldwide, and volatile organic compounds (VOCs) are its main metabolic products. The misuse or mixing of cinnamon on the market is quite serious. This study used gas chromatography-ion migration spectroscopy (GC-IMS) technology to analyze the VOCs of cinnamon samples. The measurement results showed that 66 VOCs were detected in cinnamon, with terpenes being the main component accounting for 45.45%, followed by aldehydes accounting for 21.21%. The content of esters and aldehydes was higher in RG-01, RG-02, and RG-04; the content of alcohols was higher in RG-01; and the content of ketones was higher in RG-02. Principal component analysis, cluster analysis, and partial least squares regression analysis can be performed on the obtained data to clearly distinguish cinnamon. According to the VIP results of PLS-DA, 1-Hexanol, 2-heptanone, ethanol, and other substances are the main volatile substances that distinguish cinnamon. This study combined GC-IMS technology with chemometrics to accurately identify cinnamon samples, providing scientific guidance for the efficient utilization of cinnamon. At the same time, this study is of great significance for improving the relevant quality standards of spices and guiding the safe use of spices.

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Authentication of Herbal Medicines Based on Modern Analytical Technology Combined with Chemometrics Approach: A Review

Liu,

Zuo,

et al. 2022

Critical Reviews in Analytical Chemistry

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Differentiation between wild and artificial cultivated Stephaniae tetrandrae radix using chromatographic and flow‐injection mass spectrometric fingerprints with the aid of principal component analysis

Cited by 4 publications

References 24 publications

Cordyceps cicadae Ameliorates Renal Hypertensive Injury and Fibrosis Through the Regulation of SIRT1-Mediated Autophagy

Cordyceps cicadae Ameliorates Renal Hypertensive Injury and Fibrosis Through the Regulation of SIRT1-Mediated Autophagy

Explorative Study on Volatile Organic Compounds of Cinnamon Based on GC-IMS

Authentication of Herbal Medicines Based on Modern Analytical Technology Combined with Chemometrics Approach: A Review

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