Differentiation between stains of human blood and blood of monkey

Sivaram, Sudha; Bhatnagar, Rishi; Bhandari, Sunil; Sijher, J.S.

doi:10.1016/0300-9432(75)90005-9

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…Human identification has been atOffprint requests to: T. Yokoi t e m p t e d using specific antisera with the techniques of O u c h t e r l o n y [1], E L I S A [2,3] and latex particle agglutination [4], but these techniques still have some difficulties in differentiating h u m a n s from anthropoids. Sex d e t e r m i n a t i o n has b e e n p e r f o r m e d with the techniques of quinacrine h y d o c h l o r i d e analysis of portions of Y c h r o m o s o m e [5,6] and sex h o r m o n e analysis [7].…”

Section: Introductionmentioning

confidence: 99%

Human identification and sex determination of dental pulp, bone marrow and blood stains with a recombinant DNA probe

1989

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Recombinant DNA hybridizing specifically to a 300 nucleotide repeat DNA sequence (BLUR8) of human specificity and to human repeat DNA sequence (pHY10) on the Y chromosome was used for human identification and sex determination of degraded DNA samples of blood stains, dental pulp, and bone marrow. This radioactive technique enabled reliable and sensitive human and sex determination from blood stains that were more than 80 years old. Less than 1 piece of 0.5 cm length thread of blood stain was enough for both tests. DNA from relatively fresh dental pulp and bone marrow was clearly identified. The human identification test, which could recognize up to 0.3 ng DNA correctly, was 3 to 5 times more sensitive than the sex determination test.

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Section: Introductionmentioning

confidence: 99%

Human identification and sex determination of dental pulp, bone marrow and blood stains with a recombinant DNA probe

1989

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“…Human specificity has been investigated on plasma proteins, albumin (Bauer 1974), a'2-macroglobulin (James 1965;Iwasa and Sagisaka 1979) and y-globulin (Novotny and Frank 1972;Sivaram et al 1975;Gibson 1977). These studies pointed out that human blood could not be easily distinguished from those of higher primates.…”

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confidence: 99%