Differentiation between normal renal tissue and renal tumours using functional optical coherence tomography: a phase I <i>in vivo</i> human study

Barwari, Kurdo; Bruin, Daniël M. de; Faber, Dirk J.; Leeuwen, Ton G. van; Rosette, Jean J. de la; Laguna, M. Pilar

doi:10.1111/j.1464-410x.2012.11197.x

Cited by 66 publications

(46 citation statements)

References 13 publications

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“…Also, in addition to semi-quantitative image analysis, computing the average tubular diameter and tubular volume ratio (ratio of tubular volume per volume of parenchyma) would be a more objective measure to rank the morphological parameters of the OCT data. The OCT signal attenuation has been used for the determination of malignant tissues 17 and may possibly re°ect kidney ischemic condition as well. However, Beer-Lambert law based on single-layer algorithm (which assumes homogenous tissue structures) might not work reliably on the heterogeneous OCT images we obtained.…”

Section: Discussionmentioning

confidence: 99%

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Optical coherence tomography of the living human kidney

Andrews

Wang

Wierwille

et al. 2014

J. Innov. Opt. Health Sci.

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Acute tubular necrosis (ATN) induced by ischemia is the most common insult to donor kidneys destined for transplantation. ATN results from swelling and subsequent damage to cells lining the kidney tubules. In this study, we demonstrate the capability of optical coherence tomography (OCT) to image the renal microstructures of living human donor kidneys and potentially provide a measure to determine the extent of ATN. We also found that Doppler-based OCT (i.e., DOCT) reveals renal blood°ow dynamics that is another major factor which could relate to posttransplant renal function. All OCT/DOCT observations were performed in a noninvasive, sterile and timely manner on intact human kidneys both prior to (ex vivo) and following (in vivo) their transplantation. Our results indicate that this imaging model provides transplant surgeons with an objective visualization of the transplant kidneys prior and immediately post transplantation.

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Section: Discussionmentioning

confidence: 99%

“…In a recent study of in vivo OCT human kidney, the OCT signal attenuation was used to di®erentiate malignant tissues from normal. 17 However, clinical OCT imaging of the kidney is still an under-explored area with strong translational potential.…”

Section: Introductionmentioning

confidence: 99%

Optical coherence tomography of the living human kidney

Andrews

Wang

Wierwille

et al. 2014

J. Innov. Opt. Health Sci.

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“…Recently we studied the ability of superficial OCT to differentiate between benign and malignant renal masses 8,9 . In 16 patients, intra-operative OCT measurements of tumor tissue were obtained using an externally placed OCT probe.…”

Section: Introductionmentioning

confidence: 99%

<em>In Vivo</em>, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Wagstaff

Swaan

Ingels

et al. 2015

JoVE

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Optical coherence tomography (OCT) is the optical equivalent of ultrasound imaging, based on the backscattering of near infrared light. OCT provides real time images with a 15 µm axial resolution at an effective tissue penetration of 2-3 mm. Within the OCT images the loss of signal intensity per millimeter of tissue penetration, the attenuation coefficient, is calculated. The attenuation coefficient is a tissue specific property, providing a quantitative parameter for tissue differentiation.Until now, renal mass treatment decisions have been made primarily on the basis of MRI and CT imaging characteristics, age and comorbidity. However these parameters and diagnostic methods lack the finesse to truly detect the malignant potential of a renal mass. A successful core biopsy or fine needle aspiration provides objective tumor differentiation with both sensitivity and specificity in the range of 95-100%. However, a non-diagnostic rate of 10-20% overall, and even up to 30% in SRMs, is to be expected, delaying the diagnostic process due to the frequent necessity for additional biopsy procedures.We aim to develop OCT into an optical biopsy, providing real-time imaging combined with on-the-spot tumor differentiation. This publication provides a detailed step-by-step approach for percutaneous, needle based, OCT of renal masses. Video LinkThe video component of this article can be found at

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“…Technological advancements have led to novel applications of OCT in urology, [11][12][13] gastroenterology, [14][15][16] gynecology, 17,18 dermatology, [19][20][21] cardiology, [22][23][24] and neonatology. 25 The contrast in an OCT image is based on differences in light scattering between different cellular structures in a sample.…”

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confidence: 99%

Prostate cancer diagnosis: the feasibility of needle-based optical coherence tomography

et al. 2015

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Abstract. The objective of this study is to demonstrate the feasibility of needle-based optical coherence tomography (OCT) and functional analysis of OCT data along the full pullback trajectory of the OCT measurement in the prostate, correlated with pathology. OCT images were recorded using a commercially available C7-XR™ OCT Intravascular Imaging System interfaced to a C7 Dragonfly™ intravascular 0.9-mm-diameter imaging probe. A computer program was constructed for automated image attenuation analysis. First, calibration of the OCT system for both the point spread function and the system roll-off was achieved by measurement of the OCT signal attenuation from an extremely weakly scattering medium (Intralipid® 0.0005 volume%). Second, the data were arranged in 31 radial wedges (pie slices) per circular segments consisting of 16 A-scans per wedge and 5 axial B-scans, resulting in an average A-scan per wedge. Third, the decay of the OCT signal is analyzed over 50 pixels (500 μm) in depth, starting from the first found maximum data point. Fourth, for visualization, the data were grouped with a corresponding color representing a specific μ oct range according to their attenuation coefficient. Finally, the analyses were compared to histopathology. To ensure that each single use sterile imaging probe is comparable to the measurements of the other imaging probes, the probe-to-probe variations were analyzed by measuring attenuation coefficients of 0.03, 6.5, 11.4, 17, and 22.7 volume% Intralipid®. Experiments were repeated five times per probe for four probes. Inter-and intraprobe variation in the measured attenuation of Intralipid samples with scattering properties similar to that of the prostate was <8% of the mean values. Mean attenuation coefficients in the prostate were 3.8 mmfor parts of the tissue that were classified as benign (SD: 0.8 mm −1 , minimum: 2.2 mm −1 , maximum: 8.9 mm −1 ) and 4.1 mm −1 for parts of tissue that were classified as malignant (SD: 1.2 mm −1 , minimum: 2.5 mm −1 , maximum: 9.0 mm −1 ). In benign areas, the tissue looked homogeneous, whereas in malignant areas, small glandular structures were seen. However, not all areas in which a high attenuation coefficient became apparent corresponded to areas of prostate cancer. This paper describes the first in-tissue needle-based OCT imaging and three-dimensional optical attenuation analysis of prostate tissue that indicates a correlation with pathology. Fully automated attenuation coefficient analysis was performed at 1300 nm over the full pullback. Correlation with pathology was achieved by coregistration of three-dimensional (3-D) OCT attenuation maps with 3-D pathology of the prostate. This may contribute to the current challenge of prostate imaging and the rising interest in focal therapy for reduction of side effects occurring with current therapies.

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Differentiation between normal renal tissue and renal tumours using functional optical coherence tomography: a phase I in vivo human study

Cited by 66 publications

References 13 publications

Optical coherence tomography of the living human kidney

Optical coherence tomography of the living human kidney

<em>In Vivo</em>, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Prostate cancer diagnosis: the feasibility of needle-based optical coherence tomography

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