Differentiation and secretory activities of cultured human placental cytotrophoblast

Nelson, D. F.; Rk, Meister; Ortman-Nabi, J; Sparks, Sarah J.; Vc, Stevens

doi:10.1016/s0143-4004(86)80012-1

Cited by 39 publications

(10 citation statements)

References 27 publications

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“…As depicted in Fig. 1, hCG production strongly increased after approximately 40 h and the total hCG production was comparable with previously presented data [28,29]. Together with the absence of TfRs and the fact that over 95% of the cells were hCG-positive after 40 h in culture, this was taken as evidence that the initially attached cell population was highly enriched with cytotrophoblasts.…”

Section: Vapidity Of Dna and Protein Rnea~~reme~ts As Reference Paramsupporting

confidence: 89%

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Regulation of transferrin receptor expression and distribution in in vitro cultured human cytotrophoblasts

Starreveld

Dijk

Kroos

et al. 1993

Clinica Chimica Acta

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During gestation the transplacental iron transport is very important to the fetus. Iron uptake by the placenta can be studied in cultured cytotrophoblasts. The influence of culture time and human differic transferrin on the number and distribution of transferrin receptors (TfRs) was investigated in human cytotrophoblasts. Cytotrophoblasts cultured for 2.5 h had few TfRs (0.28 pmol/mg protein). With time, total TfR amounts increase (4.14 pmol/mg protein at 70 h). They increase to a higher level in cells cultured in iron-poor medium, indicating that iron has an effect on the TfR synthesis/breakdown ratio. TfRs were distributed between two 'active' (located at the cell surface and intracellularly) and one 'inactive' (located intracellularly) receptor pools. TfR distribution among these pools was modulated by culture time and iron. Trophoblasts regulated iron uptake by variation of number of surface TfRs via changes in total TfRs and redistribution of TfRs among the receptor pools.

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Section: Vapidity Of Dna and Protein Rnea~~reme~ts As Reference Paramsupporting

confidence: 89%

“…In situ syncytiotrophoblasts differentiate out of cytotrophoblasts which are proliferative throughout gestation. During this differentiation process, cells start with the production of specific hormones (hCG, hPL and SP-1) [28,29].…”

Section: Introductionmentioning

confidence: 99%

Regulation of transferrin receptor expression and distribution in in vitro cultured human cytotrophoblasts

Starreveld

Dijk

Kroos

et al. 1993

Clinica Chimica Acta

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“…[ 26 ] Trophoblasts with high ER activity represent an active secretory subset of trophoblastic cells in Pap samples from pregnant women. [ 27 ] This distinctive feature enables the discrimination of rare fetal cells from maternal cells by simple steps of ER staining and LDA analysis. The enrichment‐free and one‐step labeling assay without cell fixation and penetration is considerably reduce damage to rare fetal cells.…”

Section: Discussionmentioning

confidence: 99%

Nondestructive Identification of Rare Trophoblastic Cells by Endoplasmic Reticulum Staining for Noninvasive Prenatal Testing of Monogenic Diseases

et al. 2020

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Noninvasive prenatal detection of monogenic diseases based on cell‐free DNA is hampered by challenges in obtaining a sufficient fraction and adequate quality of fetal DNA. Analyzing rare trophoblastic cells from Papanicolaou smears carrying the entire fetal genome provides an alternative method for noninvasive detection of monogenic diseases. However, intracellular labeling for identification of target cells can affect the quality of DNA in varying degrees. Here, a new approach is developed for nondestructive identification of rare fetal cells from abundant maternal cells based on endoplasmic reticulum staining and linear discriminant analysis (ER‐LDA). Compared with traditional methods, ER‐LDA has little effect on cell quality, allowing trophoblastic cells to be analyzed on the single‐cell level. Using ER‐LDA, high‐purity of trophoblastic cells can be identified and isolated at single cell resolution from 60 pregnancies between 4 and 38 weeks of gestation. Pathogenic variants, including –SEA/ deletion mutation and point mutations, in 11 fetuses at risk for α‐ or β‐thalassemia can be accurately detected by this test. The detection platform can also be extended to analyze the mutational profiles of other monogenic diseases. This simple, low‐cost, and noninvasive test can provide valuable fetal cells for fetal genotyping and holds promise for prenatal detection of monogenic diseases.

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“…Trophoblast isolation was performed using a modified method by Kliman et al 22 . Several authors contributed significantly to the development of the following procedure 20–29 …”

Section: Trophoblast Isolationmentioning

confidence: 99%

Comparative Effects of l‐Tryptophan and 1‐Methyl‐Tryptophan on Immunoregulation Induced by Sperm, Human Pre‐implantation Embryo and Trophoblast Supernatants

Gutiérrez

Fitzgerald

Pöhlmann

et al. 2003

American J Rep Immunol

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Problem: The hypothesis that indoleamine 2,3‐dioxygenase (IDO) is necessary to regulate lymphocyte functions at the feto‐maternal interface has been postulated, although a possible role of tryptophan (Trp) depletion in the T‐cell tolerance during insemination as well as implantation has not been previously investigated. Method of study: Allogeneic phytohaemagglutinin stimulated lymphocytes were supplemented with pre‐implantation embryo supernatant (PES), seminal plasma (SP), spermatozoa culture supernatant (SCS), spermatozoa, trophoblast cells, or placenta explant culture supernatants, and analyzed for expression of CD25, CD71, and CD69. Trp‐degrading activity was assessed by addition of 1‐methyl‐Tryptophan or l‐Trp. Results: PES, SP, trophoblast, and explant supernatants reduced the expression of CD25 in CD3 lymphocytes. Inhibition of IDO as well as Trp supplementation prevented these effects. Conclusions: These data suggest that the expression of interleukin‐2 (IL‐2) receptor in maternal T lymphocytes is normally suppressed by Trp catabolism, and that either abnormal IDO levels or substances influencing IDO activity might lead to non‐adequate immune responses on sperm, harm the conceptus or even initiate fetal rejection.

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Differentiation and secretory activities of cultured human placental cytotrophoblast

Cited by 39 publications

References 27 publications

Regulation of transferrin receptor expression and distribution in in vitro cultured human cytotrophoblasts

Regulation of transferrin receptor expression and distribution in in vitro cultured human cytotrophoblasts

Nondestructive Identification of Rare Trophoblastic Cells by Endoplasmic Reticulum Staining for Noninvasive Prenatal Testing of Monogenic Diseases

Comparative Effects of l‐Tryptophan and 1‐Methyl‐Tryptophan on Immunoregulation Induced by Sperm, Human Pre‐implantation Embryo and Trophoblast Supernatants

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