Differentiation and Radioresponse (Dose and Dose Rate) of the Primitive Germ Cell of the Bovine Testis

Erickson, B. H.; Reynolds, R. A.; Brooks, F. T.

doi:10.2307/3573497

Cited by 14 publications

(2 citation statements)

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“…Even in a strain in which radiation caused spermatogenic arrest, hormonal treatment to suppress intratesticular testosterone was able to stimulate the differentiation of endogenous surviving stem cells (Meistrich and Kangasniemi, 1997), and the same should apply to transplanted cells (Ogawa et al, 1999b; Zhang et al, 2003). Doses for killing stem cells and the subsequent repopulation of tubules have been described in the dog (Lushbaugh and Casarett, 1976), rabbit (Lyon and Cox, 1975), ram (van Vliet et al, 1988), boar (Erickson and Martin, 1984), bull (Erickson et al, 1972), rhesus macaque (van Alphen et al, 1988; van Alphen et al, 1989), cynomolgus macaque (Foppiani et al, 1999; Kamischke et al, 2003), stump‐tailed macaque (Boekelheide et al, 2005), and humans (Clifton and Bremner, 1983). For application to different species, the physical dosimetry is straightforward, and differences in pharmacokinetics, which are an issue with chemical agents such as busulfan, do not have to be considered.…”

Section: Discussionmentioning

confidence: 99%

Irradiated Mouse Testes Efficiently Support Spermatogenesis Derived From Donor Germ Cells of Mice and Rats

Zhang

Shao

Meistrich

2006

Journal of Andrology

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Testicular cell transplantation has been widely used to investigate the biology of spermatogonial stem cells, production of transgenic animals, and restoration of fertility in rodent models. One critical step in successful transplantation is the preparation of the recipient testes. Busulfan has been widely used, but irradiation has been often suggested as an alternative. There have only been limited reports of the use of irradiated animals as transplant recipients for studying differentiation of transplanted cells, and there has been no direct comparison of irradiation and busulfan as preparation methods. Mouse testes treated with local fractionated irradiation (1.5 + 12 Gy) were compared with busulfan-treated testes as recipients using mouse-to-mouse and rat-to-mouse germ cell transplantation. The fractionated irradiation schedule resulted in depletion of endogenous spermatogenesis similar to that produced by busulfan doses of 50-55 mg/kg. When immature mouse or rat testicular germ cells were transplanted into the irradiated testes, donor cells derived from either rat or mouse spermatogonial stem cells were able to form colonies of differentiated spermatogenic cells 10-13 weeks after transplantation with similar efficiencies as in busulfan-treated testes. Locally irradiated testes could be considered as an alternative to busulfan treatment for animal recipients of germ cell transplants that cannot endure the systemic toxicity of busulfan.

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Section: Discussionmentioning

confidence: 99%

Irradiated Mouse Testes Efficiently Support Spermatogenesis Derived From Donor Germ Cells of Mice and Rats

Zhang

Shao

Meistrich

2006

Journal of Andrology

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“…Transition from gonocyte to spermatogonia was shown to be accompanied by a decrease in testicular radiosensitivity of the immature rat (Hughes 1962), boar (Erickson 1964) and bull (Erickson et al 1972). In rodents, this transition is concurrent with the emergence of the germ cells from their cell cycle arrest in G0/G1 (de Rooij & Grootegoed 1998).…”

Section: Discussionmentioning

confidence: 99%

Exposure of the mouse perinatal testis to radiation leads to hypospermia at sexual maturity

Forand¹,

Messiaen²,

Habert³

et al. 2009

Reproduction

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The first round of mouse spermatogenesis begins from 3 to 4 days after birth through differentiation of gonocytes into spermatogonialstem cells and type A spermatogonia. Consequently, this step of differentiation may determine generation of the original population of stem cells and the fertility potential of the adult mouse. We aimed to determine the effect of perinatal exposure to ionizing radiation on the testis at the end of the first wave of spermatogenesis and at sexual maturity. Our results show that, radiation sensitivity of the testis substantially decreases from late foetal life to the end of the first week after birth. In addition, partial or full recovery from radiation induced testicular weight loss occurred between the first round of spermatogenesis and sexual maturity, and this was associated with the stimulation of spermatogonial proliferation. Exposure of mice at 17.5 days after conception or at 1 day after birth to g-rays decreased the sperm counts at sexual maturity, while exposure of 8 day-old mice had no effect. This suggests that irradiation of late foetal or early neonatal testes has a direct impact on the generation of the neonatal spermatogonial-stem cell pool.

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Differentiation and Growth of Cells in the Gonads

Gondos¹

1974

Differentiation and Growth of Cells in Vertebrate Tissues

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Differentiation and Radioresponse (Dose and Dose Rate) of the Primitive Germ Cell of the Bovine Testis

Cited by 14 publications

References 0 publications

Irradiated Mouse Testes Efficiently Support Spermatogenesis Derived From Donor Germ Cells of Mice and Rats

Irradiated Mouse Testes Efficiently Support Spermatogenesis Derived From Donor Germ Cells of Mice and Rats

Exposure of the mouse perinatal testis to radiation leads to hypospermia at sexual maturity

Differentiation and Growth of Cells in the Gonads

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