Differentiation ability of rat‐mesenchymal stem cells from bone marrow and adipose tissue to neurons and glial cells

Noviantari, Ariyani; Rinendyaputri, Ratih; Ariyanto, Ibnu

doi:10.22146/ijbiotech.42511

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…Guan et al (2014) also noted that using a mixture of growth factors, including bFGF, EGF, IGF-1, and NT-3, rat MSCs can differentiate into neurons [8]. showed that the addition of bFGF, EGF, and BDNF to the medium induced the differentiation of rat bone marrow MSCs into neurons and glia [19].…”

Section: Discussionmentioning

confidence: 99%

“…The immunocytochemistry staining to identify the neural-induced rat MSCs was conducted on day 14. The staining protocol was adopted by Noviantari, et al (2020) [19]. The culture medium was removed from the well-plate, then rinsed with PBS twice.…”

Section: Immunocytochemistry Staining To Identify the Neural-induced ...mentioning

confidence: 99%

“…CM from neural progenitor cells (NPC) is also reported can stimulate differentiation to astrocytes and neurons and can maintain cell growth [17,18]. showed that the addition of bFGF, EGF, and BDNF to the medium induced the differentiation of rat bone marrow MSCs into neurons and glia and characterized neural markers like A2B5, beta-III-tubulin, PSA-NCAM, and MAP-2) [19]. also reported that NT-3 role as a single neurotrophic factor in the expression of nestin in the immature neural differentiation of MSCs [20].…”

Section: Introductionmentioning

confidence: 99%

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Immunocytochemistry Studies Using Microtubule-Associated Protein-2 (MAP-2) Markers on Neural Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow

Noviantari¹,

Antarianto²,

Rif’ati³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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Mesenchymal stem cells (MSCs) can differentiate into neurons, which can be used in cell therapy. Endogenous genes and neurotrophic factors such as nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 regulate neural stem cell proliferation and differentiation (NT-3). The differentiation of MSCs into neurons is characterized by the development of neurons that interact with each other to form rings or net-like structures. The markers of neurons in the immature stage are Nestin and Vimentin. While the markers for neurons at the mature stage are neuron-specific enolase (NSE), neuronalspecific nuclear protein (NeuN), and microtubule-associated protein-2 (MAP-2). This research aims to investigate the expression of MAP-2 on the differentiation of MSCs into mature neurons. MSCs were isolated from rat bone marrow of femur and tibia using flushing methods, then cultured in Minimum Essential Medium Eagle (MEM), 10% Fetal Bovine Serum (FBS), and 1% antibiotic-antimycotic. Neuron differentiation induction medium (MEM, 2% FBS, 1% insulin-like growth factor (N2), and NT-3 20, 25, and 30 ng/mL) was used to stimulate MSCs for 14 days (control induction medium without NT-3). The immunocytochemistry of MAP-2 was performed on day 14. All experiments were done triplicated. The data obtained is the average percentage of the number of MAP-2 positive cells at each concentration. SPSS statistical analysis with a one-way ANOVA test. The results showed a significant difference between the percentage of MAP-2 positive cells at NT-3 concentrations 20 (p < 0.05), 25 (p = 0,093), 30 ng/mL (p = 0,081), and the negative control. At concentrations of NT-3 20, 25, and 30 ng/mL the

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Section: Discussionmentioning

confidence: 99%

Section: Immunocytochemistry Staining To Identify the Neural-induced ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunocytochemistry Studies Using Microtubule-Associated Protein-2 (MAP-2) Markers on Neural Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow

Noviantari¹,

Antarianto²,

Rif’ati³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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“…The coverslip was closed with a slide for further observa tion with a light microscope (Noviantari et al 2020).…”

Section: Immunocytochemical Test Against P53 Bcl-2 and Caspase-9 Prot...mentioning

confidence: 99%

Cytotoxic effects of parijoto (Medinilla speciosa Reinw. Ex. Bl.) methanol extract combined with cisplatin on WiDr colon cancer cells through apoptosis induction

Artanti

Prihapsara

Susanto

2022

Indones. J. Biotechnol.

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Parijoto (Medinilla speciosa Reinw. Ex. Bl.) is a medicinal plant with cytotoxic effects on cancer cells in vitro. As only a limited number of studies have reported the effect of parijoto on colon cancer cells, this study initially aimed to measure the total flavonoid levels and potential cytotoxic effects of parijoto methanol extract (PME) through cell viability assays and expression of the apoptotic protein on WiDr colon cancer cells as a model. PME cytotoxic activity was determined by conducting a cytotoxicity test on WiDr colon cancer cells using the MTT assay. The synergistic cytotoxic effects of the PME and cisplatin were tested to obtain the combination index (CI) value. Apoptosis was analyzed by flow cytometry, and the apoptotic protein expression was observed by immunocytochemical tests. Furthermore, quercetin as a major flavonoid in PME was measured using a UV–Vis spectrophotometer. The results showed that PME had a moderate cytotoxic activity with an IC50 of 198.64±1.6 µg/mL, whereas the IC50 of cisplatin was 2.34±0.7 µg/mL. The PME with cisplatin combination test showed a strong synergistic effect with a CI value of <1 (0.1‐0.4). The combination showed increased apoptosis properties compared to PME treatment alone. In addition, immunocytochemistry showed that PME alone or in combination with cisplatin increased the pro‐apoptosis proteins (p53 and caspase‐9) and suppressed Bcl‐2 expression. Moreover, the cell viability value increased as the PME concentration decreased. The administration of PME led to changes in cell morphology, lower cell density, and a decreasing number of living cells. Therefore, the combination of PME and cisplatin had a strong synergistic effect in inducing apoptosis.

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Therapeutical growth in oligodendroglial fate induction via transdifferentiation of stem cells for neuroregenerative therapy

et al. 2023

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Differentiation ability of rat‐mesenchymal stem cells from bone marrow and adipose tissue to neurons and glial cells

Cited by 3 publications

References 16 publications

Immunocytochemistry Studies Using Microtubule-Associated Protein-2 (MAP-2) Markers on Neural Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow

Immunocytochemistry Studies Using Microtubule-Associated Protein-2 (MAP-2) Markers on Neural Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow

Cytotoxic effects of parijoto (Medinilla speciosa Reinw. Ex. Bl.) methanol extract combined with cisplatin on WiDr colon cancer cells through apoptosis induction

Therapeutical growth in oligodendroglial fate induction via transdifferentiation of stem cells for neuroregenerative therapy

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