Differentiating Trypanosoma cruzi in a Host Mammalian Cell Imaged in Aqueous Liquid by Atmospheric Scanning Electron Microscopy

Takagi, Yuko; Sato, Mari; Naya, Masami; Sato, Chikara

doi:10.1128/spectrum.01413-21

Cited by 3 publications

(3 citation statements)

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“…The compounds were also evaluated against the trypomastigote form of Trypanosoma cruzi. Trypomastigote forms are elongated, with a kinetoplast with a rounded shape located in the region posterior to the nucleus, flagellum emerging from the flagellar pocket that is located laterally, in the posterior region of the parasite (Contreras et al 2002, Takagi et al 2022. The flagellum emerges and adheres along the body of the parasite, becoming free in the anterior region.…”

Section: Evaluation Of Cytotoxic Activity Against the Forms Of Trypan...mentioning

confidence: 99%

“…The flagellum emerges and adheres along the body of the parasite, becoming free in the anterior region. This form is highly infectious, and can be found: in the insect vector; blood and intercellular space of vertebrate hosts I have macrophages as the main host (Contreras et al 2002, Silva-Júnior et al 2022, Takagi et al 2022.…”

Section: Evaluation Of Cytotoxic Activity Against the Forms Of Trypan...mentioning

confidence: 99%

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Synthesis of 4-(4-chlorophenyl)thiazole compounds: in silico and in vitro evaluations as leishmanicidal and trypanocidal agents

Filho

Oliveira

Santos

et al. 2023

An. Acad. Bras. Ciênc.

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Neglected tropical diseases are a diverse group of communicable pathologies that mainly prevail in tropical and subtropical regions. Thus, the objective of this work was to evaluate the biological potential of eight 4-(4-chlorophenyl)thiazole compounds.Tests were carried out in silico to evaluate the pharmacokinetic properties, the antioxidant, cytotoxic activities in animal cells and antiparasitic activities were evaluated against the different forms of Leishmania amazonensis and Trypanosoma cruzi in vitro.The in silico study showed that the evaluated compounds showed good oral availability.In a preliminary in vitro study, the compounds showed moderate to low antioxidant activity. Cytotoxicity assays show that the compounds showed moderate to low toxicity.In relation to leishmanicidal activity, the compounds presented IC 50 values that ranged from 19.86 to 200 µM for the promastigote form, while for the amastigote forms, IC 50 ranged from 101 to more than 200 µM. The compounds showed better results against the forms of T. cruzi with IC 50 ranging from 1.67 to 100 µM for the trypomastigote form and 1.96 to values greater than 200 µM for the amastigote form. This study showed that thiazole compounds can be used as future antiparasitic agents.

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Section: Evaluation Of Cytotoxic Activity Against the Forms Of Trypan...mentioning

confidence: 99%

Section: Evaluation Of Cytotoxic Activity Against the Forms Of Trypan...mentioning

confidence: 99%

Synthesis of 4-(4-chlorophenyl)thiazole compounds: in silico and in vitro evaluations as leishmanicidal and trypanocidal agents

Filho

Oliveira

Santos

et al. 2023

An. Acad. Bras. Ciênc.

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“…These amastigotes undergo extensive replication before differentiating into trypomastigotes which egress by rupturing the host cell and disperse throughout the host to infect new cells 6 . A major structural reorganization of parasite cells has been described during amastigogenesis 7 , together with changes in transcriptomic and proteomic pro les 3,[8][9][10] . However, recent work indicated that some amastigotes could enter a dormant state with reduced replicative and metabolic activity, and this process has been speci cally involved in drug escape by the parasite [11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

Trypanosoma cruzi amastigote transcriptome analysis reveals heterogenous populations with replicating and dormant parasites

Desale

Herrera

Dumonteil

2023

Preprint

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Trypanosoma cruzi is a protozoan parasite causing Chagas disease, with a complex life cycle involving different stages in insect vectors and mammalian hosts. Amastigotes are an intracellular form that replicates in the cytoplasm of host cells, and recent studies suggested that dormant forms may be contributing to parasite persistence, suggesting cellular heterogeneity among amastigotes. We investigated here if a transcriptomic approach could identify some heterogeneity in intracellular amastigotes and identify a dormant population. We used gene expression data derived from bulk RNA-sequencing of T. cruzi infection of human fibrobasts for deconvolution using CDSeq, which allows to simultaneously estimate amastigote cell-type proportions and cell-type-specific expression profiles. Six amastigote subpopulations were identified, confirming intracellular amastigotes heterogeneity, and one population presented characteristics of non-replicative dormant parasites, based on replication markers and TcRAD51 expression. Transcriptomic approaches appear to be powerful to understand T. cruzi cell differentiation and expansion of these studies could provide further insight on the role different cell types in parasite persistence and Chagas disease pathogenesis.

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Trypanosoma cruzi amastigote transcriptome analysis reveals heterogenous populations with replicating and dormant parasites

Desale,

Herrera,

Dumonteil

2024

Microbes and Infection

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Differentiating Trypanosoma cruzi in a Host Mammalian Cell Imaged in Aqueous Liquid by Atmospheric Scanning Electron Microscopy

Cited by 3 publications

References 50 publications

Synthesis of 4-(4-chlorophenyl)thiazole compounds: in silico and in vitro evaluations as leishmanicidal and trypanocidal agents

Synthesis of 4-(4-chlorophenyl)thiazole compounds: in silico and in vitro evaluations as leishmanicidal and trypanocidal agents

Trypanosoma cruzi amastigote transcriptome analysis reveals heterogenous populations with replicating and dormant parasites

Trypanosoma cruzi amastigote transcriptome analysis reveals heterogenous populations with replicating and dormant parasites

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