Differentiating effects of murine nerve growth factor in the peripheral and central nervous systems of Xenopus laevis tadpoles.

Levi‐Montalcini, Rita; Aloe, Luigi

doi:10.1073/pnas.82.20.7111

Cited by 54 publications

(6 citation statements)

References 18 publications

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“…Some earlier studies also showed that mouse submandibular glands synthesize and release into the saliva large quantities of NGF and that synthesis of this protein is controlled by testosterone and thyroxine [reviewed in 48 ]. Later, Levi-Montalcini and Aloe found that systemic murine NGF injections lead to growth and differentiation of sensory and sympathetic nerve cells as well as on several populations of cells in the central nervous system of Xenopus laevis tadpoles [49] . Using animal model of aggression Spillantini et al detected increased NGF mRNA and protein production in the hypothalamus [50] .…”

Section: Discussionmentioning

confidence: 99%

Nerve growth factor and its receptor in schizophrenia

et al. 2014

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Promising studies suggest that defects in synaptic plasticity detected in schizophrenia may be linked to neurodevelopmental and neurodegenerative abnormalities and contribute to disease-associated cognitive impairment. We aimed to clarify the role of the synaptic plasticity regulatory proteins, nerve growth factor (NGF) and its receptor (NGFR) in the pathogenesis of schizophrenia by comparative analysis of their blood levels and functional single nucleotide polymorphisms (SNPs) in genes encoding these proteins (NGF and NGFR) in schizophrenia-affected and healthy subjects. Relationships between the selected SNPs' genotypes and NGF and NGFR plasma levels were also assessed. Our results demonstrated a positive association between schizophrenia and the NGF rs6330 as well as the NGFR rs11466155 and rs2072446 SNPs. Also, a negative association between this disorder and NGF rs4839435 as well as NGFR rs734194 was found. In both, haloperidol-treated and antipsychotic-free patients decreased blood levels of the NGF and NGFR were found, and a positive interrelation between rs6330 and rs2072446 carriage and decreased NGF and NGFR levels, respectively, was revealed. In conclusion, our results demonstrate association of schizophrenia with the rs6330, rs4839435 and rs734194, rs11466155, rs2072446 as well as with the decreased blood levels of corresponding proteins. Our findings indicate the implication of alterations in NGFR and NGFR genes in schizophrenia, particularly, in defects of synaptic plasticity. Furthermore, the data obtained suggests that at least in Armenian population the NGF rs6330*T and NGFR rs11466155*T, rs2072446*T alleles might be nominated as risk factors, whereas the NGF rs4839435*A and NGFR rs734194*G alleles might be protective against developing schizophrenia.

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Section: Discussionmentioning

confidence: 99%

Nerve growth factor and its receptor in schizophrenia

et al. 2014

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“…While NGF is the prototypical neurotrophic and survival factor for both sympathetic and sensory neurons during development [6], it is involved in inflammatory hyperalgesia in adulthood [7], [8]. Previous study from our laboratory has shown NGF is produced in the muscle after LC and plays a pivotal role in DOMS [4].…”

Section: Introductionmentioning

confidence: 97%

TRPV1 and TRPV4 Play Pivotal Roles in Delayed Onset Muscle Soreness

et al. 2013

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Unaccustomed strenuous exercise that includes lengthening contraction (LC) often causes tenderness and movement related pain after some delay (delayed-onset muscle soreness, DOMS). We previously demonstrated that nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) are up-regulated in exercised muscle through up-regulation of cyclooxygenase (COX)-2, and they sensitized nociceptors resulting in mechanical hyperalgesia. There is also a study showing that transient receptor potential (TRP) ion channels are involved in DOMS. Here we examined whether and how TRPV1 and/or TRPV4 are involved in DOMS. We firstly evaluated a method to measure the mechanical withdrawal threshold of the deep tissues in wild-type (WT) mice with a modified Randall-Selitto apparatus. WT, TRPV1−/− and TRPV4−/− mice were then subjected to LC. Another group of mice received injection of murine NGF-2.5S or GDNF to the lateral gastrocnemius (LGC) muscle. Before and after these treatments the mechanical withdrawal threshold of LGC was evaluated. The change in expression of NGF, GDNF and COX-2 mRNA in the muscle was examined using real-time RT-PCR. In WT mice, mechanical hyperalgesia was observed 6–24 h after LC and 1–24 h after NGF and GDNF injection. LC induced mechanical hyperalgesia neither in TRPV1−/− nor in TRPV4−/− mice. NGF injection induced mechanical hyperalgesia in WT and TRPV4−/− mice but not in TRPV1−/− mice. GDNF injection induced mechanical hyperalgesia in WT but neither in TRPV1−/− nor in TRPV4−/− mice. Expression of NGF and COX-2 mRNA was significantly increased 3 h after LC in all genotypes. However, GDNF mRNA did not increase in TRPV4−/− mice. These results suggest that TRPV1 contributes to DOMS downstream (possibly at nociceptors) of NGF and GDNF, while TRPV4 is located downstream of GDNF and possibly also in the process of GDNF up-regulation.

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“…Monoclonal antibodies (192-IgG; a generous gift from E. M. Johnson, Jr., Washington University, Saint Louis) were radioiodinated with Na1251 (Amersham) by the chloramine-T procedure (22)(23)(24), and 1SI-labeled antibodies (125I-192-IgG)…”

Section: Methodsmentioning

confidence: 99%

Adrenalectomy decreases nerve growth factor in young adult rat hippocampus.

Aloe

1989

Proc. Natl. Acad. Sci. U.S.A.

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The effect of adrenalectomy on the level of nerve growth factor (NGF) in the hippocampus and on the distribution of choline acetyltransferase i ureactivity in forebrain cholinergic neurons of developing rats was studied. Biological and immunohistochemical determinations indicated that in 40-day-old rats, adrenalectomy reduced the NGF level in the hippocampus and the choline acetyltrsferase immunoreactivity in the septal lateral bands. Furthermore, autoradiographic studies showed that adrenalectomy causes changes in the distribution and expression of NGF receptors in the hippocampus. These results suggest that adrenal hormones are involved in the regulation of the NGF level in the hippocampus and of NGF receptors in the septum.Nerve growth factor (NGF) is a polypeptide found in both the peripheral (1) and the central (2) nervous system. In the rat central nervous system, the hippocampus contains one of the highest levels of NGF and of mRNA encoding NGF (2-5). Several lines of evidence indicate that NGF is synthesized within the hippocampus, taken up selectively by cholinergic neurons of the septal lateral band (SLB) terminals, and transported retrogradely to the cell of origin (6-9). Intrahippocampal injection of NGF antibodies causes granule cell loss (10), further suggesting that NGF plays a physiological role in the central nervous system. The hippocampus is a major target site of glucocorticoid hormones, particularly corticosterone, and administration ofglucocorticoids early in development influences brain and somatic growth (11). Furthermore, recent in vitro and in vivo studies have shown that glucocorticoids have a profound influence on the survival of hippocampal neurons (12). Whether or not these hormonal activities affect the level of NGF in the hippocampus and the distribution of NGF receptors in the basal forebrain cholinergic neurons has yet to be explored. The present studies show that in developing rats, adrenalectomy significantly reduces the level of NGF in the hippocampus and of choline acetyltransferase (ChAT) immunoreactivity in the cholinergic neurons of the SLB while enhancing a concomitant increase of NGF receptor expression in the septal neurons. A preliminary account of these studies has been presented.* MATERIALS AND METHODSAnimals and Adrenalectomy. Forty-day-old SpragueDawley rats (90-100 g) were purchased from Nossan (Calco, Italy). Rats were subjected either to bilateral adrenalectomy or to sham operation (laparotomy) under ether anesthesia. Corticosterone (Sigma) was dissolved in ethanol/saline, 1:9 (vol/vol), and injected intradermally into the adrenalectomized (Adx) rats at doses of 2 gg every other day for 2 weeks. Adx rats were given 0.9% NaCl in place of drinking water to compensate for the loss of salt.Mouse NGF (2.5S) was purified from male mouse submaxillary glands (13). Polyclonal NGF antibodies were prepared (14,15) and purified by affinity chromatography (16).Biological Assays. At intervals, control and treated rats were killed with an overdose of ether vapor and ...

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Differentiating effects of murine nerve growth factor in the peripheral and central nervous systems of Xenopus laevis tadpoles.

Cited by 54 publications

References 18 publications

Nerve growth factor and its receptor in schizophrenia

Nerve growth factor and its receptor in schizophrenia

TRPV1 and TRPV4 Play Pivotal Roles in Delayed Onset Muscle Soreness

Adrenalectomy decreases nerve growth factor in young adult rat hippocampus.

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