Differentiating breast cancer molecular subtypes using a DNA aptamer selected against MCF-7 cells

Liu, Mei; Yang, Tong; Chen, Zhongsi; Wang, Zhifei; He, Nongyue

doi:10.1039/c8bm00787j

Cited by 45 publications

(18 citation statements)

References 51 publications

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“…In addition, for patients with inaccessible lesions, the injection of tracer into the PT needs to be completed with the assistance of an imaging personnel. Therefore, we believe that tracer injection into the PT requires knowledgeable medical staff, which complicates the biopsy procedure [24][25][26][27][28][29][30][31][32][33][34][35]. When comparing different injection sites in this study, it was found that the detection performance of the three tracers, MB, 99mTc-PHY, and nanocarbon, injected in the SA was better than that injected in the PT.…”

Section: Detection Values Of Different Tracers For Slns By Slnbmentioning

confidence: 76%

Nanocarbon Tracer and Areola Injection Site Are Superior in the Sentinel Lymph Node Biopsy Procedure for Breast Cancer

Song¹,

Zhang²,

An³

et al. 2022

Computational and Mathematical Methods in Medicine

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Background. Axillary lymph node (ALN) staging is the most effective method to evaluate the condition of patients with breast cancer, their choice of treatment options, and prognosis. The sentinel lymph node (SLN) status assessment is the key to sentinel lymph node biopsy (SLNB) in patients with breast cancer. The choice of tracer and tracer injection sites affects SLNB. Objective. This study mainly analyzes the best tracer for SLNB and the best choice of tracer injection site. Methods. A total of 165 breast cancer patients who underwent SLNB were selected and injected with methylene blue or 99mTc-labeled sodium phytate or nanocarbon 20 min before biopsy. The number of SLNs detected by different tracers in different injection sites such as peritumoral tissue (PT) and subareolar area (SA) was counted, and the sensitivity, specificity, and positive/negative prediction rates were recorded and compared. Results. The detection success rate, average detection number of SLNs, and detection accuracy of the nanocarbon tracer were higher than the other two. The detection sensitivity, specificity, and positive and negative prediction rates of nanocarbon for SLNB were also higher than those of the other two tracers. When comparing the performance of tracers in different injection sites, it was found that the detection of three tracers injected in the SA was better than the injection in the PT. Conclusion. For women with early-stage breast cancer, nanocarbon can be used as the preferred tracer for SLNB to determine the status of the patient’s ALNs, and the areola area can be used as the best injection site.

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Section: Detection Values Of Different Tracers For Slns By Slnbmentioning

confidence: 76%

Nanocarbon Tracer and Areola Injection Site Are Superior in the Sentinel Lymph Node Biopsy Procedure for Breast Cancer

Song¹,

Zhang²,

An³

et al. 2022

Computational and Mathematical Methods in Medicine

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“…In vivo imaging of the MCF-7 specific aptamer with a K d of 82.25 ± 25.14 nM was performed analogous to sk6Ea and revealed moderate tumor uptake in MCF-7 xenografts but no uptake in SK-BR-3 or MDA-MB-231 tumors 47 . In a follow up study, MF3 was truncated to generate MF3Ec with a 4-fold lower K d of 18.95 ± 2.9 nM against MCF-7 cells 48 .…”

Section: Molecular Imaging For the Characterization Of Novel Aptamersmentioning

confidence: 99%

Aptamers used for molecular imaging and theranostics - recent developments

Bohrmann¹,

Burghardt²,

Haynes³

et al. 2022

Theranostics

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Aptamers are single stranded oligonucleotides that fold into three dimensional structures and are able to recognize a variety of molecular targets. Due to the similarity to antibodies with regards to specificity and affinity and their chemical versatility, aptamers are increasingly used to create targeted probes for in vivo molecular imaging and therapy. Hence, aptamer-based probes have been utilized in practically all major imaging modalities such as nuclear imaging, magnetic resonance imaging, x-ray computed tomography, echography and fluorescence imaging, as well as newer modalities such as surface enhanced Raman spectroscopy. Aside from targeting, aptamers have been used for the creation of sensors that allow the localized detection of cellular markers such as ATP in vivo . This review focuses on in vivo studies of aptamer-based probes for imaging and theranostics since the comprehensive overview by Bouvier-Müller and Ducongé in 2018.

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“…Next, EDC and NHS were used to activate the free carboxyl group on the MoS 2 -BSA surface, and subsequently aptamers were modified to obtain composite MoS 2 -BSA-Apt nanosheets, which were stable and biocompatible. Owing to the good affinity between aptamers and receptors on the cell surface [ 74 , 75 ], the composition would distinguish MCF-7 human breast cancer cells from other cells and then enter the cells through endocytosis. Under the irradiation from an 808 nm laser, the heat generated kills cancer cells, which is on the basis of MoS 2 nanosheets.…”

Section: Aptamer-bound Nanomaterials Used In Different Cancer Therapiesmentioning

confidence: 99%

Applications of Aptamer-Bound Nanomaterials in Cancer Therapy

et al. 2021

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Cancer is still a major disease that threatens human life. Although traditional cancer treatment methods are widely used, they still have many disadvantages. Aptamers, owing to their small size, low toxicity, good specificity, and excellent biocompatibility, have been widely applied in biomedical areas. Therefore, the combination of nanomaterials with aptamers offers a new method for cancer treatment. First, we briefly introduce the situation of cancer treatment and aptamers. Then, we discuss the application of aptamers in breast cancer treatment, lung cancer treatment, and other cancer treatment methods. Finally, perspectives on challenges and future applications of aptamers in cancer therapy are discussed.

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Differentiating breast cancer molecular subtypes using a DNA aptamer selected against MCF-7 cells

Abstract: Aptamers are single-stranded DNA or RNA oligonucleotides selected by systematic evolution of ligands by exponential enrichment (SELEX), which show great potential in the diagnosis and personalized therapy of cancers, due to their specific advantages over antibodies.

Cited by 45 publications

References 51 publications

Nanocarbon Tracer and Areola Injection Site Are Superior in the Sentinel Lymph Node Biopsy Procedure for Breast Cancer

Nanocarbon Tracer and Areola Injection Site Are Superior in the Sentinel Lymph Node Biopsy Procedure for Breast Cancer

Aptamers used for molecular imaging and theranostics - recent developments

Applications of Aptamer-Bound Nanomaterials in Cancer Therapy

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