Differentiating Among Plant Spectra by Combining pH Dependent Photoluminescence Spectroscopy with Multi-Way Principal Component Analysis (MPCA)

Abstract

Photoluminescence spectroscopic probes offer the potential for differentiation among plant species in real-time. Since pH has been shown to be pivotal in affecting extraction efficiency of other plant compounds pH seemed appropriate as an additional dimension within our multi-way principal component analysis (MPCA) to differentiate among six different plant species. In particular, MPCA allowed differentiation between Sporobolus and Pleuraphis that was not possible using only principal component analysis (PCA). This research suggests MPCA may be a more appropriate tool than PCA when attempting to discriminate among plant species.

Fluorescence, electrophoretic and chromatographic fingerprints of herbal medicines and their comparative chemometric analysis

“…These include high performance liquid chromatography (HPLC) combined with mass spectrometry (MS) [1,2] or UV-Vis spectrophotometric detection [3,4] , gas chromatography (GC) [5,6], capillary electrophoresis (CE) [7][8][9], paper-based colorimetric assays [10,11], and infrared [12] and fluorescence spectroscopy [13]. Nowadays, there is an increasing need to exploit methods that are quick, reliable and efficient and, moreover, can be easily automated for on-site analysis.…”