Differentiated tonsil-derived mesenchymal stem cells embedded in Matrigel restore parathyroid cell functions in rats with parathyroidectomy

Sustained release of parathyroid hormone via in situ cross‐linking gelatin hydrogels improves the therapeutic potential of tonsil‐derived mesenchymal stem cells for hypoparathyroidism

“…Better yet would be need‐based control of PTH release, which would truly restore the lost functions of parathyroid glands. In this regard, stem cell‐based therapies, such as TMSC‐based parathyroid regeneration (Park et al , ), are promising for structural and functional recovery of target tissue (Saraswati et al , ; Yu et al , ). In particular, we have shown that TMSCs can function as ‘smart cells’ capable of responding to extracellular calcium levels (Park et al , ), secreting more iPTH when the surrounding calcium concentration is lower.…”

“…In this regard, stem cell‐based therapies, such as TMSC‐based parathyroid regeneration (Park et al , ), are promising for structural and functional recovery of target tissue (Saraswati et al , ; Yu et al , ). In particular, we have shown that TMSCs can function as ‘smart cells’ capable of responding to extracellular calcium levels (Park et al , ), secreting more iPTH when the surrounding calcium concentration is lower. Nonetheless, massive losses of implanted stem cells and the subsequent reduction in therapeutic efficacy remains a major hurdle to implementing this approach (Hong et al , ; Lee et al , 2014), one that we previously tackled by embedding TMSCs in a Matrigel scaffold by simple physical mixing to regenerate parathyroid tissue (Park et al , ).…”

“…In particular, we have shown that TMSCs can function as ‘smart cells’ capable of responding to extracellular calcium levels (Park et al , ), secreting more iPTH when the surrounding calcium concentration is lower. Nonetheless, massive losses of implanted stem cells and the subsequent reduction in therapeutic efficacy remains a major hurdle to implementing this approach (Hong et al , ; Lee et al , 2014), one that we previously tackled by embedding TMSCs in a Matrigel scaffold by simple physical mixing to regenerate parathyroid tissue (Park et al , ). Only when embedded in Matrigel did dTMSCs show parathyroid‐specific functions in a PTX rat model, controlling serum iPTH and iCa 2+ concentrations.…”

“…We recently described new mesenchymal stem cells derived from human tonsillar tissues, reporting that these tonsil‐derived mesenchymal stem cells (TMSCs) were a source of PTH (Park et al , ). As we noted in this report, differentiated TMSCs (dTMSCs) were capable of restoring in vivo parathyroid function only if embedded in a Matrigel (MA) scaffold.…”

Preventive effects of tonsil‐derived mesenchymal stem cells on osteoradionecrosis in a rat model

“…We previously isolated stem cells from palatine tonsillar tissue, and demonstrated that tonsil‐derived MSCs can differentiate parathyroid tissue, which is associated with bone formation. They can also regulate immune responses . Other studies suggested that bone marrow‐derived mesenchymal stem cells (BMMSCs) had a therapeutic effect on ORN in swine and rat models .…”