Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer

Sung, Li Ying; Gao, Shaorong; Shen, Hongmei; Yu, Hui; Song, Yifang; Smith, Sadie; Chang, Ching-Chien; Inoue, Kimiko; Kuo, Lynn; Jin, Lian; Li, Ao; Tian, X. Cindy; Tuck, David; Weissman, Sherman M.; Yang, Xusan; Cheng, Tao

doi:10.1038/ng1895

Cited by 112 publications

(71 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, human peripheral blood granulocytes and mononuclear cells, including monocytes, NK cells and T cells, express CRISPLD2 that was identified by RT-PCR (Fig. 2C) in concordance with published microarray data for gene expression (35)(36)(37)(38). CRISPLD2 was readily seen in the cytoplasm of PBMCs (Fig.…”

Section: Tissue Expression and Serum Concentrationsupporting

confidence: 66%

The Novel Lipopolysaccharide-Binding Protein CRISPLD2 Is a Critical Serum Protein to Regulate Endotoxin Function

Wang

Xing

Fan

et al. 2009

The Journal of Immunology

View full text Add to dashboard Cite

LPS is an immunostimulatory component of Gram-negative bacteria. Acting on the immune system in a systemic fashion, LPS exposes the body to the hazard of septic shock. In this study we report that cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2/Crispld2; human and mouse/rat versions, respectively), expressed by multitissues and leukocytes, is a novel LPS-binding protein. As a serum protein, median CRISPLD2 concentrations in health volunteers and umbilical cord blood samples are 607 μg/ml and 290 μg/ml, respectively. Human peripheral blood granulocytes and mononuclear cells including monocytes, NK cells, and T cells spontaneously release CRISPLD2 (range, 0.2–0.9 μg/ml) and enhance CRISPLD2 secretion (range, 1.5–4.2 μg/ml) in response to stimulation of both LPS and humanized anti-human TLR4-IgA Ab in vitro. CRISPLD2 exhibits significant LPS binding affinity similar to that of soluble CD14, prevents LPS binding to target cells, reduces LPS-induced TNF-α and IL-6 production, and protects mice against endotoxin shock. In in vivo experiments, serum Crispld2 concentrations increased in response to a nontoxic dose of LPS and correlated negatively with LPS lethality, suggesting that CRISPLD2 serum concentrations not only are indicators of the degree of a body’s exposure to LPS but also reflect an individual’s LPS sensitivity.

show abstract

Section: Tissue Expression and Serum Concentrationsupporting

confidence: 66%

The Novel Lipopolysaccharide-Binding Protein CRISPLD2 Is a Critical Serum Protein to Regulate Endotoxin Function

Wang

Xing

Fan

et al. 2009

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…As shown in the table, although the developmental potential was higher in bovine compared with mice, the overall conclusion is that cloning efficiency is similar among somatic cell types. Although some types of stem cell, such as bone marrow mesenchymal stem cells (Kato et al, 2004), neural stem cells , and hematopoietic stem cells (Sung et al, 2006), might be adequate for cloning, the results do not indicate equivalent efficiency suggesting that the low efficiency of SCNT must not depend on the cell type, but that ooplasm alone is also not sufficient for complete reprogramming of the somatic nucleus. To improve the efficiency of SCNT cloning, several possibilities are considered.…”

Section: Donor Cell Typementioning

confidence: 59%

Role of the donor nuclei in cloning efficiency: can the ooplasm reprogram any nucleus?

Kato¹,

Tsunoda²

2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Cloning efficiency has not been dramatically improved after the first success of somatic cell nuclear transfer (SCNT) in sheep in 1997. The reasons for the low efficiency of SCNT embryos must be attributed to the insufficient reprogramming of the donor nucleus in ooplasm. It has been clarified that the methylation and acetylation status are disordered in SCNT embryos and the gene expression pattern is different and widely varied in SCNT embryos, compared with fertilized embryos. In this paper, we focused on the role of the donor nuclei in cloning efficiency, and discuss whether ooplasm can reprogram any nucleus.

show abstract

“…The higher appropriate remodeling of stem cell-NT derived embryos has proved in different species (Bosch et al, 2006, Faast et al, 2006, Kumar et al, 2012, Lee et al, 2006, Rideout et al, 2000. Some reports, however, have rejected transcendence of stem cells over somatic cells in normal reprogramming of NT derived embryos (Berg et al, 2007, Li et al, 2013, Sung et al, 2006. In the first report on the use of bovine MSCs for NT, no priority in developmental potential was observed in stem cell derived embryos compared to non-stem cell derived counterparts (Kato et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

Nazari¹,

Shirazi²,

Shams‐Esfandabadi³

et al. 2016

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Nuclear reprogramming of a differentiated cell in somatic cell nuclear transfer (SCNT) is a major concern in cloning procedures. Indeed, the nucleus of the donor cell often fails to express the genes which are a prerequisite for normal early embryo development. This study was aimed to evaluate the developmental competence and the expression pattern of some reprogramming related genes in bovine cloned embryos reconstructed with amniotic membrane stem cells (AMSCs) in comparison with those reconstructed with mesenchymal stem cells (MSCs) and adult fibroblasts (AF) as well as with in vitro fertilized (IVF) oocytes. In vitro matured abattoir-derived oocytes were considered as recipients and a hand-made cloning technique was employed for oocyte enucleation and nuclear transfer (NT) procedures. The expression pattern of genes involved in self-renewal and pluripotency (POU5F1, SOX2, NANOG), imprinting (IGF2, IGF2R), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), and apoptosis (BAX, BCL2) were evaluated in NT and IVF derived embryos. Despite the insignificant difference in cleavage rate between reconstructed and IVF oocytes, the blastocyst rate in the IVF group was higher than that of other groups. Among reconstructed oocytes, a higher blastocysts rate was observed in MSC-NT and AMSCs-NT derived embryos that were significantly higher than AF-NT derived ones. There were more similarities in the expression pattern of pluripotency and epigenetic modification genes between MSC-NT and IVF derived blastocysts compared with other groups. In conclusion, considering developmental competence, AMSCs, as alternative donors in SCNT procedure, like MSCs, were prone to have more advantage compared with AF.

show abstract

Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer

Cited by 112 publications

References 30 publications

The Novel Lipopolysaccharide-Binding Protein CRISPLD2 Is a Critical Serum Protein to Regulate Endotoxin Function

The Novel Lipopolysaccharide-Binding Protein CRISPLD2 Is a Critical Serum Protein to Regulate Endotoxin Function

Role of the donor nuclei in cloning efficiency: can the ooplasm reprogram any nucleus?

The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

Contact Info

Product

Resources

About