Differentially expressed proteins in positive versus negative HNSCC lymph nodes

Abstract

BackgroundLymph node metastasis is one of the most important prognostic factors in head and neck squamous cell carcinomas (HNSCCs) and critical for delineating their treatment. However, clinical and histological criteria for the diagnosis of nodal status remain limited. In the present study, we aimed to characterize the proteomic profile of lymph node metastasis from HNSCC patients.MethodsIn the present study, we used one- and two-dimensional electrophoresis and mass spectrometry analysis to characterize the proteomic profile of lymph node metastasis from HNSCC.ResultsComparison of metastatic and non-metastatic lymph nodes showed 52 differentially expressed proteins associated with neoplastic development and progression. The results reinforced the idea that tumors from different anatomical subsites have dissimilar behaviors, which may be influenced by micro-environmental factor including the lymphatic network. The expression pattern of heat shock proteins and glycolytic enzymes also suggested an effect of the lymph node environment in controlling tumor growth or in metabolic reprogramming of the metastatic cell. Our study, for the first time, provided direct evidence of annexin A1 overexpression in lymph node metastasis of head and neck cancer, adding information that may be useful for diagnosing aggressive disease.ConclusionsIn brief, this study contributed to our understanding of the metastatic phenotype of HNSCC and provided potential targets for diagnostic in this group of carcinomas.Electronic supplementary materialThe online version of this article (10.1186/s12920-018-0382-6) contains supplementary material, which is available to authorized users.

A Chemical Probe for Tudor Domain Protein Spindlin1 to Investigate Chromatin Function

“…47 Inflammation within the tumor microenvironment (cancer-related inflammation (CRI)) is now known to be a significant contributing factor in the development and progression of cancer, particularly in tumor invasion and metastasis. 44,48 Of the 50 genes identified here, a number have previously been reported to be differentially expressed (GBP2, 49 DUOX2, 50 IGFBP3 [51][52] ), or were associated with a more invasive and metastatic phenotype (AKR1B10, 53 FABP5, [53][54] Importantly, compound 35 seems to modulate these genes in a direction which opposes that identified in these previously reported studies, thus may be of therapeutic value.…”

“…Of the 50 genes identified here, a number have previously been reported to be differentially expressed (GBP2, DUOX2, IGFBP3 , ) or were associated with a more invasive and metastatic phenotype (AKR1B10, FABP5, , HSPB1, KRT1, S100A9, CNFN, ID2, LCN2 (NGAL), IGFBP2, IFIT1, CCL5 ,, ) in squamous cell carcinomas (SCC).…”

“…HSPB1,53 KRT1, 53 S100A9,53 CNFN, 55 ID2, 56 LCN2 (NGAL), 57 IGFBP2, 52 IFIT1, 58 CCL548,[59][60] ) in squamous cell carcinomas (SCC).…”

A Chemical Probe For Tudor Domain Protein Spindlin1 to Investigate Chromatin Functions

“…47 Inflammation within the tumor microenvironment (cancer-related inflammation (CRI)) is now known to be a significant contributing factor in the development and progression of cancer, particularly in tumor invasion and metastasis. 44,48 Of the 50 genes identified here, a number have previously been reported to be differentially expressed (GBP2, 49 DUOX2, 50 IGFBP3 [51][52] ), or were associated with a more invasive and metastatic phenotype (AKR1B10, 53 FABP5, [53][54] HSPB1, 53 KRT1, 53 S100A9, 53 CNFN, 55 ID2, 56 LCN2 (NGAL), 57 IGFBP2, 52 IFIT1, 58 CCL5 48,[59][60] ) in squamous cell carcinomas (SCC). Importantly, compound 35 seems to modulate these genes in a direction which opposes that identified in these previously reported studies, thus may be of therapeutic value.…”

Enhancing the Bystander and Abscopal Effects to Improve Radiotherapy Outcomes

“…On the other hand, the overexpression of this molecule has been reported in many cancers, although its clinical meaning is still controversial (140)(141)(142), which could be, in part, due to the localization of ANXA1 in the nuclear and cytoplasmic compartments, and also associated to the membrane (131). In fact, the expression level of ANXA1 is down-regulated in numerous types of cancer and is linked with metastasis, relapse, and poor prognosis (141,143); ANXA1 is an endogenous inhibitor of NF-κB that may be stimulated in human cancer cells and in experimental mice models by powerful anti-inflammatory glucocorticoids and altered by non-steroidal anti-inflammatory drugs (143).…”