Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi

Abstract

Background Verrucous epidermal naevi (VEN) are benign skin tumours, considered keratinocytic epidermal naevi, that appear at birth or early childhood. VEN may display a range of appearances, depending on patient age. Although the number of studies regarding VEN is increasing, the exact mechanism of VEN is still unknown.Objectives The aim of this study was to analyse the changes in the expression of protein factors in lesions of VEN children by TMT labelling-based quantitative proteomics.Methods A total of 8 children with VEN (5 for experiment and 3 for validation) and 8 healthy children (5 for experiment and 3 for validation) presented to the Department of Dermatology, the First Affiliated Hospital of Anhui Medical University, Boao Super Hospital, between January 2019 and November 2019. The lesions and lesion-adjacent tissues from children with VEN and naevus-adjacent normal skin tissues from children with pigmented naevi were defined as the VEN group, VENC group and C group, respectively. We performed a proteomics analysis to screen for differentially expressed proteins in the lesions of these individuals. We further performed Western blotting to validate the relative expression levels of nine targeted proteins in the validation group.Results According to the proteomics results, a total of 4970 proteins were identified, and 4770 proteins were quantified. Among these proteins, 586 proteins were up-or downregulated at least 1.3-fold with a P-value < 0.05 (upregulated: 399, downregulated: 187) in lesions between the VEN group and the C group. These proteins played important roles in multiple biological functions, such as cornification, epidermal cell differentiation and neutrophil activation, and formed a complicated protein-protein interaction network. Of the 586 up-or downregulated proteins, nine were selected for further validation. According to Western blotting analysis results, the relative expression levels of Involucrin, NDUFA4, Loricrin, Keratin type II cytoskeletal 6A (Cytokeratin 6A), BRAF, Filaggrin, S100A7 and Desmocollin-3 were significantly upregulated in VEN children and may be associated with skin barrier dysfunction, epidermal cell overgrowth and differentiation, inflammation and immune and oxidative phosphorylation, which are involved in the pathogenesis of VEN.Conclusions According to TMT-based proteomics and Western blotting results, we identified eight noteworthy proteins, Involucrin, NDUFA4, Loricrin, Keratin type II cytoskeletal 6A, BRAF, Filaggrin, S100A7 and Desmocollin-3, that were upregulated in the lesions of VEN children and may be associated with the pathogenesis of VEN. Our findings provide new starting points for identifying precise pathogenic mechanisms or therapeutic targets for VEN.

LncRNA ENST00000370438 promotes cell proliferation by upregulating DHCR24 in breast cancer

“…To identify ENST00000370438 regulated proteins, we determined the differences in the proteomic profile between BC cells transfected with control and ENST00000370438 siRNAs using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) as previously described 24 . Next, we performed bioinformatics analysis.…”

“…To identify ENST00000370438 regulated proteins, we determined the differences in the proteomic profile between BC cells transfected with control and ENST00000370438 siRNAs using liquid chromatographytandem mass spectrometry (LC-MS/MS) as previously described. 24 Next, we performed bioinformatics analysis. Differentially expressed proteins between two groups were determined using the Wilcoxon test, and the threshold values were set as p < 0.05 and 1.3-fold change.…”

Comparative Proteomic Analysis of Liver Tissues and Serum in db/db Mice

“…Recently, TMT (tandem mass tag)-labeling proteomic analysis has been recommended as a dependable method for accurately quantifying relative protein levels in complex samples due to its technical strengths such as good sensitivity, reproducibility, and signal-to-noise ratio [ 11 , 12 ]. Ample studies have employed this method to discover potential biomarkers that are closely correlated to the phenotypes of diseases including NAFLD [ 10 , 13 , 14 , 15 , 16 ].…”

Effect of aluminium concentration on phase formation and radiation stability of Cr2Al x C thin film

“…Synthesis of Cr2AlC ternary compositions can be done in bulk form by hot pressing, spark plasma sintering, and pressureless sintering [10,11]. Cr2AlC has also been produced as thin films [12][13][14][15] by magnetron sputtering from elemental or composite targets.…”

“…During the synthesis of Cr2AlC ternary carbides, both in bulk form and thin films, the formation of secondary phases [11,[17][18][19][20][21] like Cr2Al, Al8Cr5, Cr7C3, and γ -Cr2AlC have been reported. In the case of the bulk Cr2AlC [10,11,[22][23][24], the crystallinity variations were attributed partially to synthesis parameters like temperature and homogeneity of raw powder mixtures. It is well known that the formation of various phases in Cr2AlC is not too dissimilar to all MAX phase family of materials and it is well known.…”