DIFFERENTIALLY EXPRESSED MicroRNAs IN SMALL CELL LUNG CANCER

Mikó, Edit; Czimmerer, Zsolt; Csánky, Eszter; Boros, Gábor; Buslig, Julia; Dezső, Balázs; Scholtz, Beáta

doi:10.3109/01902140902822312

Cited by 113 publications

(86 citation statements)

References 49 publications

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“…Expression alterations for the 3 miRNAs with increased expression found in the blood samples in this study were reported in connection with other carcinomas. Lung cancer showed alterations in miR-26b and miR-374 (21,22). Changes in miR-144 expression levels were observed in hepatocellular, lung and prostate cancer tissues (23).…”

Section: Discussionmentioning

confidence: 95%

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

et al. 2013

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Abstract. Since differential expression of microRNAs (miRNAs) has been found to be highly associated with several types of cancer, the goal of the present study was to identify an miRNA fingerprint as a non-invasive diagnostic tool to detect urinary bladder cancer using the easily accessible samples of whole blood and urine. Blood and urine samples from 4 controls and from patients suffering from superficial and invasive bladder cancer were analyzed using miRNA microarray consisting of 754 human miRNAs from the Sanger database v14. Using RT-qPCR technique, 6 of the differentially expressed miRNAs were validated in the controls (20 blood, 19 urine samples) and patients with superficial (18 blood, 16 urine samples) or invasive (20 blood and urine samples each) tumours. Three blood miRNAs (miR-26b-5p, miR-144-5p, miR-374-5p) were found to be significantly upregulated in invasive bladder tumour patients (P<0.05) when compared to the control group. The expression of 2 miRNAs (miR-618, miR-1255b-5p) in the urine of patients with invasive tumours was significantly (P<0.05) increased in comparison to the control group. Blood miR-26b-5p detected the presence of invasive bladder tumours with 94% specificity and 65% sensitivity. The urine miR-1255b-5p reached 68% specificity and 85% sensitivity in the diagnosis of invasive tumours. This pilot study represents the first characterization of an miRNA profile for urinary bladder tumours in whole blood samples. In addition, it was shown that invasive bladder tumours could be identified by differentially expressed urine miRNAs. Further studies are needed to test the clinical usefulness for bladder cancer detection and surveillance.

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Section: Discussionmentioning

confidence: 95%

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

et al. 2013

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“…Since our previous work has shown that miR-126 is uniformly under-expressed in primary SCLC tumors [10], in the present study we investigated its role in regulating the proliferation of SCLC cells.…”

Section: Introductionmentioning

confidence: 99%

miR-126 inhibits proliferation of small cell lung cancer cells by targeting SLC7A5

Margitai²,

et al. 2011

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Edited by Tamas Dalmay Keywords:Small cell lung cancer miR-126 SLC7A5 G1 delay a b s t r a c t Despite intensive efforts to improve therapies, small cell lung cancer (SCLC) still has a dismal median survival of 18 months. Since miR-126 is under-expressed in the majority of SCLC tumors, we investigated the effect of miR-126 overexpression on the proliferation and cell cycle distribution of H69 cells. Our results demonstrate that miR-126 inhibits proliferation of H69 cells, by delaying the cells in the G1 phase. Short interfering RNA (siRNA) mediated suppression of SLC7A5, a predicted target of mir-126, has the same effect on H69 cells. We also show for the first time that SLC7A5 is a direct target of miR-126.

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“…Miko et al (24) employed microarray and RT-qPCR analyses to determine the miRNA expression profile in primary SCLC, and the result revealed that at least 24 miRNAs were differentially expressed between the normal lung and primary SCLC tumor tissues. A previous study performed gene expression profiling of drug-resistant SCLC cells by combining miRNA and cDNA expression analyses, and identified 61 significantly differentially expressed miRNAs (25).…”

Section: Discussionmentioning

confidence: 99%

“…The functions of the targets of these miRNAs were involved in organ development, tissue differentiation, cell apoptosis and defense, signal transduction and the electron transfer chain. For example, the target of hsa-miR-29 encodes vascular endothelial growth factor A, which has various effects, including mediating increased vascular Glutamate receptor, metabotropic 5, coiled-coil domain containing 67, fucosyltransferase 9 (α (1,3) fucosyltransferase), H3 histone, family 3B hsa-miR- 24 Calcitonin receptor, mannose-P-dolichol utilization defect 1, kinase suppressor of ras 2, CKLF-like MARVEL transmembrane domain containing 4, spermatogenesis associated, serine-rich 2-like has-miR-25 CD69 molecule, folliculin interacting protein 1, actin, α, cardiac muscle 1; F-box and WD repeat domain containing 7; aspartate β-hydroxylase hsa-miR-29 PIK3R1, Vascular endothelial growth factor A, PIK3RZ hsa-miR- 30 MAPKS, NRG3, KRAS, PIK3CD, RARB, KRAS, CCNEZ, ITGA6, peroxisome proliferator-activated receptor γ, coactivator 1β, makorin ring finger protein 3 hsa-miR-34…”

Section: Annotation Of the Targets Of Mirnas Involved In Sclcmentioning

confidence: 99%

Genome-wide identification and expression analysis of microRNA involved in small cell lung cancer via deep sequencing

Yan

Shi

Wang

et al. 2014

Molecular Medicine Reports

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Abstract. Small cell lung cancer is a major cause of mortality worldwide. microRNAs (miRNAs) are involved in various biological processes through regulating gene expression. In the present study, to identify the miRNAs involved in human small cell lung cancer at the genome-wide level, Solexa sequencing was employed to sequence two small RNA (sRNA) libraries from small cell lung cancer tissues (LC sRNA library) and the corresponding normal tissues (NT sRNA library). Deep sequencing of the two sRNA libraries identified a number of conserved miRNAs and differential expression analysis of these miRNAs revealed 81 miRNAs differentially expressed in small cell lung cancer, of which more than half were downregulated. The expression trends determined by sequencing were validated by reverse transcription-quantitative polymerase chain reaction analysis. The annotations for the targets of these miRNAs were predicted. This study provides valuable information for understanding the regulatory mechanisms of miRNAs involved in human small cell lung cancer. IntroductionMicroRNAs (miRNAs) are small non-coding RNA molecules, ~22 nucleotides long, which are widely distributed in plants and animals (1,2). The primary miRNA transcripts are cleaved by Drosha and Dicer enzymes to form mature miRNAs, which serve as posttranscriptional negative gene regulators by either cleaving mRNA or inhibiting translation (3,4). Through such mechanisms, miRNAs are involved in various biological processes, including organ development, tissue differentiation, cell cycle regulation and cancer development (4-6).Lung cancer is the predominant cause of cancer-related mortality worldwide and its pathogenesis is closely associated with tobacco smoking. Lung cancer is categorized into two main histological groups: Non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). Regulation of miRNAs is closely associated with tumor initiation, promotion and progression (7). Recently, increasing evidence has demonstrated that miRNAs may be associated with lung cancer. A previous study revealed that an increase in hsa-miR-196a expression levels was a characteristic molecular change in NSCLC (8,9), and that hsa-miRNA-196a promoted NSCLC cell proliferation and invasion via targeting HOXA5 (10). Overexpression of miR-200 was shown to reduce the expression levels of NSCLC prognostic biomarkers in H1299 and BEAS-2B cells (11). miR-449a, which inhibited migration and invasion through targeting c-Met, was found to be downregulated in NSCLC tissues and cell lines (12). Reduced expression of miR-101 was associated with overexpression of EZH2 and exhibited tumor-suppressive functions in NSCLC (13). This evidence suggests that miRNAs are crucial in NSCLC; however, little is known regarding the functions and regulation of miRNAs in SCLC.In the present study, Solexa sequencing was used to generate a large quantity of small RNA (sRNA) data for SCLC and corresponding normal tissues. Sequence analysis was employed to identify specific miRNAs associated with SCLC and these ...

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DIFFERENTIALLY EXPRESSED MicroRNAs IN SMALL CELL LUNG CANCER

Cited by 113 publications

References 49 publications

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

miR-126 inhibits proliferation of small cell lung cancer cells by targeting SLC7A5

Genome-wide identification and expression analysis of microRNA involved in small cell lung cancer via deep sequencing

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