Differentially Expressed lncRNAs in Gastric Cancer Patients: A Potential Biomarker for Gastric Cancer Prognosis

Abstract

Current studies indicate that long non-coding RNAs (lncRNAs) are frequently aberrantly expressed in cancers and implicated with prognosis in gastric cancer (GC). We intended to generate a multi-lncRNA signature to improve prognostic prediction of GC. By analyzing ten paired GC and adjacent normal mucosa tissues, 339 differentially expressed lncRNAs were identified as the candidate prognostic biomarkers in GC. Then we used LASSO Cox regression method to build a 12-lncRNA signature and validated it in another independent GEO dataset. An innovative 12-lncRNA signature was established, and it was significantly associated with the disease free survival (DFS) in the training dataset. By applying the 12-lncRNA signature, the training cohort patients could be categorized into high-risk or low-risk subgroup with significantly different DFS (HR = 4.52, 95%CI= 2.49-8.20, P < 0.0001). Similar results were obtained in another independent GEO dataset (HR=1.58, 95%CI=1.05 - 2.38, P=0.0270). Further analysis showed that the prognostic value of this 12-lncRNA signature was independent of AJCC stage and postoperative chemotherapy. Receiver operating characteristic (ROC) analysis showed that the area under receiver operating characteristic curve (AUC) of combined model reached 0.869. Additionally, a well-performed nomogram was constructed for clinicians. Moreover, single-sample gene-set enrichment analysis (ssGSEA) showed that a group of pathways related to drug resistance and cancer metastasis significantly enriched in the high risk patients. A useful innovative 12-lncRNA signature was established for prognostic evaluation of GC. It might complement clinicopathological features and facilitate personalized management of GC.

Long noncoding RNA NEAT1‐modulated miR‐506 regulates gastric cancer development through targeting STAT3

“…20 Hence, it is crucial to explore the underlying mechanism of lncRNAs in GC progression. 20 Hence, it is crucial to explore the underlying mechanism of lncRNAs in GC progression.…”

A prognostic 3‐long noncoding RNA signature for patients with gastric cancer

“…Recent studies demonstrated that the deregulated lncRNA expression profiles were related to the progression and survival in patients with various cancers including GC. 9 However, whether an lncRNA signature derived from one or more lncRNAs can predict long-term outcomes in patients with GC is unknown.…”

Identification of dysregulated long noncoding RNA and associated mechanism in gastric cancer

“…Among all identi ed studies, differences in lncRNAs expression levels were detected for at least 75 lncRNAs [12]. Because of the re-annotation of the published microarray database, two studies did not provide fold changes of dysregulated lncRNAs [12,14,15]. A study from Hu et al used a 1.5-fold change for selection criteria [16], while 2 fold change was used for other studies [12,[17][18][19][20][21][22][23][24].…”