Differentially expressed late constituents of the epidermal cornified envelope

Marshall, Damian; Hardman, Matthew J.; Nield, Kerry; Byrne, Carolyn

doi:10.1073/pnas.231489198

Cited by 152 publications

(146 citation statements)

References 28 publications

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“…20,21 Cytokeratin 13 (Tables 1 and 2) was found at high levels only in murine aorta. Other known CE precursor proteins, such as involucrin, filaggrin, elafin, annexin I, repetin, cystatin A, trichohyalin, and loricrin, 28 were not detected in murine aorta or IVC with the microarray analysis or by qRT-PCR (data not shown).…”

Section: Common Features Of Cornified Cell Envelope In Skin and Aortamentioning

confidence: 95%

Enrichment of Genes in the Aortic Intima That Are Associated With Stratified Epithelium

et al. 2005

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Background-Arteries and veins are exposed to different pressures and are easily distinguished by morphology. Although several recent studies have focused on differential gene expression between the arterial and venous endothelium, the molecular distinctions that give rise to the dramatic structural distinctions between arteries and veins, such as in the organization of the intima, are not known. Methods and Results-We used high-density oligonucleotide arrays to analyze the transcriptional profile of the mouse aorta and inferior vena cava (IVC), not restricting our analysis to the endothelium, to identify genes whose expression was enriched in aorta over other tissues and the IVC. By quantitative reverse transcription-polymerase chain reaction analysis, these genes have been shown to be highly expressed in the mouse aorta and were either expressed at low levels or were undetectable in the murine IVC. By immunofluorescence analysis of human tissue, we determined that a subset of these aorta-enriched proteins exhibited a primarily intima-restricted expression. Intimal expression of at least a subset of these genes, plakoglobin, galectin 7, sciellin, and SPRR3, was also detected in other types of arteries but not in veins. Furthermore, SPRR3 expression in the intima was primarily associated with atheromas. The proteins identified are functionally related in that they are known to also be enriched in stratified epithelia, where they play an important role in stress-bearing and barrier properties. Conclusions-Vascular expression of these genes has not been reported previously. Our observations suggest that they may play a significant role in the mechanisms by which large arteries may adapt to biomechanical stress.

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Section: Common Features Of Cornified Cell Envelope In Skin and Aortamentioning

confidence: 95%

Enrichment of Genes in the Aortic Intima That Are Associated With Stratified Epithelium

et al. 2005

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“…PLA2 g (PLA2G4C), group IIF phospholipase (PLA2G2F) and group IIE phospholipase (PLA2G2E) induce the release of arachidonate from phospholipids. [15][16][17] LEP14 and LEP13 18 were predicted in silico to have PLA2 activity, and PATE bears a putative PLA2 motif. 19 Adiponutrin is similar to patatin-like phospholipase domain-containing protein 3 and also shows PLA2 activity.…”

Section: Lipases and Lipase Activity Associated Genes Are Overexpressmentioning

confidence: 99%

Targeting lipid metabolism by the lipoprotein lipase inhibitor orlistat results in apoptosis of B-cell chronic lymphocytic leukemia cells

et al. 2007

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Constitutively activated pathways contribute to apoptosis resistance in chronic lymphocytic leukemia (CLL). Little is known about the metabolism of lipids and function of lipases in CLL cells. Performing gene expression profiling including B-cell receptor (BCR) stimulation of CLL cells in comparison to healthy donor CD5 þ B cells, we found significant overexpression of lipases and phospholipases in CLL cells. In addition, we observed that the recently defined prognostic factor lipoprotein lipase (LPL) is induced by stimulation of BCR in CLL cells but not in CD5 þ normal B cells. CLL cellular lysates exhibited significantly higher lipase activity compared to healthy donor controls. Incubation of primary CLL cells (n ¼ 26) with the lipase inhibitor orlistat resulted in induction of apoptosis, with a halfmaximal dose (IC 50 ) of 2.35 lM. In healthy B cells a significantly higher mean IC 50 of 148.5 lM of orlistat was observed, while no apoptosis was induced in healthy peripheral blood mononuclear cells (PBMCs; Po0.001). Orlistat-mediated cytotoxicity was decreased by BCR stimulation. Finally, the cytotoxic effects of orlistat on primary CLL cells were enhanced by the simultaneous incubation with fludarabine (P ¼ 0.003). In summary, alterations of lipid metabolism are involved in CLL pathogenesis and might represent a novel therapeutic target in CLL.

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“…To confirm the expression levels of all genes identified in this analysis, Q-RT-PCR was performed on independently isolated samples (Table 1). Of the 28 genes identified as up-regulated in K5-Klf4 and Dex-treated mouse skin and down-regulated in Klf4 Ϫ/Ϫ mouse skin, 5 (Filaggrin, Lce2, Lce3, Lce5, and Lce7) map to the epidermal differentiation complex, a tandem array of genes encoding proteins that are cross-linked to form the proteinaceous component of the barrier (17). We have studied the coordinate gene regulation of the epidermal differentiation complex in Klf4 Ϫ/Ϫ mice (18).…”

Section: Identification Of Developmental Targets Of Corticosteroids Amentioning

confidence: 99%

Klf4 and corticosteroids activate an overlapping set of transcriptional targets to accelerate in utero epidermal barrier acquisition

Patel

Seo

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Premature infants are at an increased risk for infections and dehydration because of incomplete development of the epidermis, which attains its essential function as a barrier only during the last stages of in utero development. When a premature birth is anticipated, antenatal corticosteroids are administered to accelerate lung epithelium differentiation. One pleiotropic, but beneficial, effect of antenatal corticosteroids is acceleration of skin barrier establishment by an unknown mechanism. In mice, the transcription factor Klf4 is both necessary and sufficient, within a developmental field of competence, to establish this skin barrier, as demonstrated by targeted ablation and transgenic expression of Klf4, respectively. Here, we report that Klf4 and corticosteroid treatment coordinately accelerate barrier acquisition in vivo. Transcriptional profiling reveals that the genes regulated by corticosteroids and Klf4 during the critical window of epidermal development significantly overlap. KLF4 activates the proximal promoters of a significant subset of these genes. Dissecting the intersection of the genetic and pharmacological pathways, regulated by KLF4 and corticosteroids, respectively, leads to a mechanistic understanding of the normal process of epidermal development in utero.development ͉ transcription factor ͉ skin ͉ glucocorticoid receptor

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Differentially expressed late constituents of the epidermal cornified envelope

Cited by 152 publications

References 28 publications

Enrichment of Genes in the Aortic Intima That Are Associated With Stratified Epithelium

Enrichment of Genes in the Aortic Intima That Are Associated With Stratified Epithelium

Targeting lipid metabolism by the lipoprotein lipase inhibitor orlistat results in apoptosis of B-cell chronic lymphocytic leukemia cells

Klf4 and corticosteroids activate an overlapping set of transcriptional targets to accelerate in utero epidermal barrier acquisition

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