Differentially expressed circular RNAs in systemic lupus erythematosus and their clinical significance

Zhang, Mingyue; Wang, Jie‐Bing; Zhu, Zhiwei; Li, Lian-Ju; Liu, Ruishan; Yang, Xiao‐Ke; Leng, Rui‐Xue; Li, Xiaomei; Pan, Hai‐Feng; Ye, Dong‐Qing

doi:10.1016/j.biopha.2018.08.161

Cited by 33 publications

(32 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They could competitively bind to the miRNA and then decrease the inhibitory effect of the miRNA on the expression of target genes 23, 24. Many studies have proved that circRNAs are potential biomarkers and participate in pathogenesis of various autoimmune diseases 25, 26, 27. For instance, decreased hsa_circ_0044235 in PBMCs has been confirmed to have significant value in the diagnosis of RA.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA Expression Profiling and the Potential Role of hsa_circ_0089172 in Hashimoto’s Thyroiditis via Sponging miR125a-3p

Xiong

Peng

Ding

et al. 2019

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

Circular RNA (circRNA) is a novel subclass of noncoding-RNA molecules that participate in development and progression of a variety of human diseases via sponging microRNAs (miRNAs), but the role of circRNAs in Hashimoto's thyroiditis (HT) has not been defined. In this study, peripheral blood samples from five patients with HT and five healthy volunteers were investigated by Illumina HiSeq Sequencer. A total of 627 differentially expressed circRNAs including 370 upregulated and 257 downregulated ones were identified in HT patients. Four upregulated circRNAs indicated the same rising tendency toward sequencing results. The expression of hsa_circ_0089172 was upregulated and correlated positively with the serum level of the thyroid peroxidase antibody. Two perfectly matched binding sites of miR-125a-3p were found in hsa_circ_0089172 sequences with bioinformatics tools. The expression of miR-125a-3p was decreased in the HT patients and correlated inversely with an elevated level of hsa_circ_0089172. Moreover, knockdown of hsa_circ_0089172 resulted in an increase of the expression of miR-125a-3p in vitro. Receiver operating characteristic (ROC) curve analysis suggested that hsa_circ_0089172 had significant value in HT diagnosis. Taken together, these results demonstrate that hsa_circ_0089172 as a potential diagnostic biomarker of HT and may play a crucial role in the pathogenesis of HT via sponging miR-125a-3p.

show abstract

Section: Discussionmentioning

confidence: 99%

Circular RNA Expression Profiling and the Potential Role of hsa_circ_0089172 in Hashimoto’s Thyroiditis via Sponging miR125a-3p

Xiong

Peng

Ding

et al. 2019

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

show abstract

“…Thus, it was speculated that the dE circRNAs or miRNAs associated with blood loss could also be correlated with ONFH. Third, the dE circRNAs and miRNAs might also result from the autoimmune diseases such as SLE instead of ONFH, which has been demonstrated in previous studies (44)(45)(46). It is true that SLE itself could lead to DE miRNAs and circRNAs.…”

Section: Discussionmentioning

confidence: 75%

Changed cellular functions and aberrantly expressed miRNAs and circRNAs in bone marrow stem�cells in osteonecrosis of the femoral head

2020

View full text Add to dashboard Cite

The present study aimed to detect the correlations between altered cellular functions in bone marrow stem cells (BMScs) and osteonecrosis of the femoral head (ONFH). By profiling the aberrant expression of miRNAs and circRNAs in BMScs isolated from ONFH patients, the present study aimed to further explore the potential regulatory mechanisms of action of circRNAs in ONFH using integrated bioinformatics analysis. BMScs were isolated from seven ONFH patients and seven controls. Cellular functions, including proliferation, apoptosis and differentiation, were compared. miRNA and circRNA sequencing were conducted using RNA samples of three ONFH patients and three controls to identify differentially expressed circRNAs and miRNAs. The expression of hsa_circ_0000219, hsa_circ_0004588 and hsa_circ_0005936 were validated by qPCR. Target miRNAs were also predicted and validated by qPCR and circRNA-miRNA co-expression networks were constructed. BMScs of ONFH patients displayed decreased proliferation and increased apoptosis during in vitro culturing. In addition, reduced osteogenesis and enhanced adipogenesis were found in the ONFH group. A total of 129 miRNAs and 231 circRNAs were detected to be differentially expressed. The expression levels of hsa_circ_0000219, hsa_circ_0004588 and hsa_circ_0005936 were significantly decreased in BMSCs of ONFH patients. A number of target miRNAs related to cell proliferation, apoptosis and differentiation were predicted for hsa_circ_0000219 and hsa_circ_0005936. The expression levels of miR-144-3p and miR-1270 were found to be elevated in ONFH patients, which was consistent with miRNA sequencing data and competitive endogenous RNA hypothesis. Time-dependent expression patterns of hsa_circ_0000219, hsa_circ_0004588, hsa_circ_0005936, miR-144-3p and miR-1270 were also validated during osteogenic and adipogenic differentiation in BMSCs. The results of the present study substantiated the involvement of BMSCs in ONFH development. hsa_circ_0000219 and hsa_circ_0005936 may regulate the progression of ONFH by mediating the proliferation and differentiation of BMSCs by sponging miRNAs.

show abstract

“…These properties indicate that the potential of circRNAs to be ideal biomarkers for human diseases (21). Moreover, the diagnostic performance of plasma or PBMC circRNA has been examined in SLE (38,39). However, to the best of our knowledge, the expression profiles and diagnostic performance of circulating circRNAs in peripheral blood from patients with SLE have been rarely reported, and there was previously only one paper investigating circRNAs expression in peripheral blood from patients with SLE (40).…”

Section: Discussionmentioning

confidence: 99%

Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus

Luo

et al. 2020

Mol Med Rep

View full text Add to dashboard Cite

Circular RNAs (circRNAs) have gained attention due to their performance in disease diagnosis. However, the characteristics of circRNAs in peripheral blood from patients with systemic lupus erythematosus (SLE) remain unknown. Therefore, the aim of the present study was to determine the expression profile and diagnostic potential of circRNAs in peripheral blood from patients with SLE. The global circRNA expression in the peripheral blood of patients with SLE and healthy controls (HCs) was detected using a circRNA microarray. Then, the expression levels of three upregulated circRNAs were selected for further validation by reverse transcription-quantitative PCR (RT-qPCR) in a training set. Moreover, the diagnostic value of these circRNAs was assessed by constructing a receiver operating characteristic curve, and then verified in a blind testing set. In total, 1,566 circRNAs were identified to be dysregulated between patients with SLE and HCs (≥2 fold change, P<0.05). Furthermore, the RT-qPCR results were consistent with the microarray data, in that all three selected circRNAs, hsa_circ_0082688, hsa_circ_0082689 and hsa_circ_0008675, were significantly upregulated in patients with SLE (P<0.05). Results from the training set demonstrated that the combination of hsa_circ_0082688-hsa_circ_0082689 may provide the most beneficial diagnostic potential. Moreover, the blind test results indicated that the combination model of hsa_circ_0082688-hsa_circ_0082689 could effectively discriminate between patients with SLE from patients with rheumatoid arthritis and HCs, with a sensitivity of 91.30%, a specificity of 78.57% and an accuracy of 82.28%. Moreover, the combination model of hsa_circ_0082688-hsa_circ_0082689 + anti-dsDNA could more effectively discriminated the SLE group from the control groups, with a sensitivity of 95.65%, a specificity of 100.00% and an accuracy of 98.73%. In addition, correlation analysis results suggested that all three circRNAs in patients with SLE did not correlate with the SLE disease activity index. In conclusion, the expression levels of hsa_circ_0082688-hsa_circ_0082689 may serve as potential biomarkers for SLE diagnosis.

show abstract

Differentially expressed circular RNAs in systemic lupus erythematosus and their clinical significance

Cited by 33 publications

References 40 publications

Circular RNA Expression Profiling and the Potential Role of hsa_circ_0089172 in Hashimoto’s Thyroiditis via Sponging miR125a-3p

Circular RNA Expression Profiling and the Potential Role of hsa_circ_0089172 in Hashimoto’s Thyroiditis via Sponging miR125a-3p

Changed cellular functions and aberrantly expressed miRNAs and circRNAs in bone marrow stem�cells in osteonecrosis of the femoral head

Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus

Contact Info

Product

Resources

About