Differential Transport of Fertilized and Unfertilized Ova in the Rat

Villalón, Manuel; Ortiz, Mauricio; Aguayo, Claudio; Muñoz, Javiera; Croxatto, Horacio B.

doi:10.1095/biolreprod26.2.337

Cited by 33 publications

(14 citation statements)

References 6 publications

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“…Presumably, as ova entered the uterus prematurely, they were lost through the vagina. This has been reported to be the case in rats [5,27,28]. Thrombocytopenia was evident in pregnant but not in pseudopregnant or cycling females.…”

Section: Discussionmentioning

confidence: 72%

Possible Role of Platelet-Activating Factor in Embryonic Signaling during Oviductal Transport in the Hamster1

Velásquez

Aguilera

Croxatto³

1995

Biology of Reproduction

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Hamster embryos enter the uterus in pregnant females nearly one day earlier than unfertilized eggs in cycling females. The hypothesis that a substance derived from eicosanoids is released by the embryos, but not by oocytes, to hasten their transport to the uterus was tested by examining the effects of indomethacin, nordihydroguaiaretic acid (NDGA), platelet-activating factor (PAF), and PAF antagonists on egg transport in the hamster. Administration of indomethacin had no effect on embryo transport, whereas administration of NDGA delayed the transport of eggs to the uterus in pregnant but not in cycling hamsters. The PAF antagonists TCV-309 and BN-52021 delayed significantly the transport of eggs to the uterus in pregnant animals, but not in cycling animals; i.e., they retarded the passage of embryos but not of oocytes to the uterus. Administration of PAF to cycling hamsters hastened the oviductal transport of ova. These data suggest that, in the hamster, the earlier passage of embryos to the uterus as compared to oocytes is mediated by PAF. Thrombocytopenia was detected in early-pregnant hamsters, and PAF-like activity was detected in spent media of two-cell through morula stage hamster embryos. These results suggest that preimplantation hamster embryos produce PAF-like activity that mediates embryonic signaling to the oviduct as well as pregnancy-associated thrombocytopenia.

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Section: Discussionmentioning

confidence: 72%

Possible Role of Platelet-Activating Factor in Embryonic Signaling during Oviductal Transport in the Hamster1

Velásquez

Aguilera

Croxatto³

1995

Biology of Reproduction

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“…Several studies indicated that horse embryos produced prostaglandin E 2 (PGE 2 ), which mediates an acceleration of the transit to the uterus (Weber et al 1991a,b). In rats and hamsters, fertilized eggs reach the uterus at higher rates compared with the unfertilized eggs (Villalon et al 1982, Ortiz et al 1986). These findings illustrate that embryo transport is an interactive process between the embryos and the oviduct.…”

Section: Embryo Transportmentioning

confidence: 99%

“…COH is one of the common hormonal regimens used to induce ovulation, either alone or as part of the IVF/ICSI procedures (Farhi & Orvieto 2010, Berker et al 2011. Studies using in vitro maturated (IVM) human oocytes (retrieved from gonadotropin-stimulated patients) demonstrate that the wildly used oocyte morphological maturation protocol may not necessarily indicate an adequate maturation of gene expression (Jones et al 2008, Virant-Klun et al 2013. These genes are involved in meiosis (SYCP2, SGOL2 and MSH2) and are upregulated in the IVM oocytes compared with in vivo maturated oocytes.…”

Section: Epigeneticsmentioning

confidence: 99%

Oviduct: roles in fertilization and early embryo development

Winuthayanon

2017

Journal of Endocrinology

202

191

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Animal oviducts and human Fallopian tubes are a part of the female reproductive tract that hosts fertilization and pre-implantation development of the embryo. With an increasing understanding of roles of the oviduct at the cellular and molecular levels, current research signifies the importance of the oviduct on naturally conceived fertilization and pre-implantation embryo development. This review highlights the physiological conditions within the oviduct during fertilization, environmental regulation, oviductal fluid composition and its role in protecting embryos and supplying nutrients. Finally, the review compares different aspects of naturally occurring fertilization and assisted reproductive technology (ART)-achieved fertilization and embryo development, giving insight into potential areas for improvement in this technology.

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“…No differences have been found in rabbits (Hodgson and Pauerstein, 1976), whereas in hamsters, a marked difference in the oviductal transport of embryos and unfertilized ova has been noted (Ortiz et al, 1986). In rats, in turn, a different transport of both types of ova has been clearly observed in the uterus, but not in the oviduct (Villaldn et al, 1982). This suggested that in rats only the uterus is able to recognize differences between fertilized and unfertilized ova, or that the differences appear only at more advanced stages of preimplantation development.…”

Section: Introductionmentioning

confidence: 99%

Embryos of Different Ages Transferred to the Rat Oviduct Enter the Uterus at Different Times1

Ortiz

Llados

Croxatto

1989

Biology of Reproduction

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Indirect evidence of embryo signalling to the oviduct was sought in rats by examining the transport of embryos of different ages. One-cell or four-cell embryos were transferred to the oviducts of recipient rats on Day 1 of pregnancy, and the number, condition, and location of native and transferred embryos was assessed on Day 4. To control for the effect of the presence of foreign embryos and excess number of eggs and the transfer procedure upon the fate of native embryos, other groups of rats were sham-operated or left undisturbed. Recipients had a mean number of ova significantly higher than controls. In controls and recipients of 1-cell embryos, the majority of eggs reached the morula stage and all of them were located in the oviducts. In those animals receiving 4-cell embryos, half of the eggs had reached the blastocyst stage and 28% were in the uteri (p less than 0.005). These results support the idea that advanced embryos can influence the timing of their entrance to the uterus in rats.

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Differential Transport of Fertilized and Unfertilized Ova in the Rat

Abstract: A possible role of the quality of eggs in the regulation of ovum transport was investigated in pseudopregnant rats. Pseudopregnancy was induced

Cited by 33 publications

References 6 publications

Possible Role of Platelet-Activating Factor in Embryonic Signaling during Oviductal Transport in the Hamster1

Possible Role of Platelet-Activating Factor in Embryonic Signaling during Oviductal Transport in the Hamster1

Oviduct: roles in fertilization and early embryo development

Embryos of Different Ages Transferred to the Rat Oviduct Enter the Uterus at Different Times1

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