Differential transcriptional activity of kidney genes in hypertensive ISIAH and normotensive WAG rats

Redina, Olga E.; Smolenskaya, S. É.; Абрамова, Т. О.; Иванова, Л. Н.; Markel, A. L.

doi:10.3109/10641963.2014.954711

Cited by 10 publications

(5 citation statements)

References 64 publications

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“…In the current study, we found multiple DEGs related to response to different stimuli and to stress. This is well consistent with the previous observation that HPA and sympathoadrenal systems are activated in ISIAH rats [ 11 ] and that the changes in kidney function of 6-month old ISIAH rats are based on altered expression of many genes working in stress-related mode [ 19 ]. The stress response (or stress cascade) is considered as disruptions in homeostasis which result in a series of neural and endocrine adaptations.…”

Section: Discussionsupporting

confidence: 92%

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Comparative transcriptional profiling of renal cortex in rats with inherited stress-induced arterial hypertension and normotensive Wistar Albino Glaxo rats

et al. 2016

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BackgroundThe renal function plays a leading role in long-term control of arterial pressure. The comparative analysis of renal cortex transcriptome in ISIAH rats with inherited stress-induced arterial hypertension and normotensive WAG rats was performed using RNA-Seq approach. The goal of the study was to identify the differentially expressed genes (DEGs) related to hypertension and to detect the pathways contributing to the differences in renal functions in ISIAH and WAG rats.ResultsThe analysis revealed 716 genes differentially expressed in renal cortex of ISIAH and WAG rats, 42 of them were associated with arterial hypertension and regulation of blood pressure (BP). Several Gene Ontology (GO) terms significantly enriched with DEGs suggested the existence of the hormone dependent interstrain differences in renal cortex function. Multiple DEGs were associated with regulation of blood pressure and blood circulation, with the response to stress (including oxidative stress, hypoxia, and fluid shear stress) and its regulation. Several other processes which may contribute to hypertension development in ISIAH rats were: ion transport, regulation of calcium ion transport, homeostatic process, tissue remodeling, immune system process and regulation of immune response.KEGG analysis marked out several pathways significantly enriched with DEGs related to immune system function, to steroid hormone biosynthesis, tryptophan, glutathione, nitrogen, and drug metabolism.ConclusionsThe results of the study provide a basis for identification of potential biomarkers of stress-sensitive hypertension and for further investigation of the mechanisms that affect renal cortex function and hypertension development.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-015-0306-9) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

“…So, the decreased expression of Comt in the renal cortex of ISIAH rats may lead to increase in renal dopaminergic effects and sodium excretion, and may be considered as an adaptive mechanism. Earlier, the significantly decreased transcription of Comt was also detected in kidney of 6-month old ISIAH rats [ 19 ].…”

Section: Discussionmentioning

confidence: 92%

Comparative transcriptional profiling of renal cortex in rats with inherited stress-induced arterial hypertension and normotensive Wistar Albino Glaxo rats

et al. 2016

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“…So, the decreased expression of Comt in the renal medulla of ISIAH rats may lead to increase in renal dopaminergic effects and sodium excretion, and may be considered as an adaptive or protective mechanism for control of hypertension development in ISIAH rats. Earlier, the significantly decreased transcription of Comt was also detected in kidney of 6-month old ISIAH rats [53]. …”

Section: Discussionmentioning

confidence: 92%

The gene-expression profile of renal medulla in ISIAH rats with inherited stress-induced arterial hypertension

et al. 2016

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BackgroundThe changes in the renal function leading to a reduction of medullary blood flow can have a great impact on sodium and water homeostasis and on the long-term control of arterial blood pressure. The RNA-Seq approach was used for transcriptome profiling of the renal medulla from hypertensive ISIAH and normotensive WAG rats to uncover the genetic basis of the changes underlying the renal medulla function in the ISIAH rats being a model of the stress-sensitive arterial hypertension and to reveal the genes which possibly may contribute to the alterations in medullary blood flow.ResultsMultiple DEGs specifying the function of renal medulla in ISIAH rats were revealed. The group of DEGs described by Gene Ontology term ‘oxidation reduction’ was the most significantly enriched one. The other groups of DEGs related to response to external stimulus, response to hormone (endogenous) stimulus, response to stress, and homeostatic process provide the molecular basis for integrated responses to homeostasis disturbances in the renal medulla of the ISIAH rats. Several DEGs, which may modulate the renal medulla blood flow, were detected. The reduced transcription of Nos3 pointed to the possible reduction of the blood flow in the renal medulla of ISIAH rats.ConclusionsThe generated data may be useful for comparison with those from different models of hypertension and for identifying the common molecular determinants contributing to disease manifestation, which may be potentially used as new pharmacological targets.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-016-0462-6) contains supplementary material, which is available to authorized users.

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“…Comparative analysis of gene expression profiling in kidney of hypertensive ISIAH and normotensive WAG rats revealed 126 differentially expressed genes in renal cortex and 65 differentially expressed genes in renal medulla [ 12 ]. The hierarchical clustering and heatmaps illustrating each individual's expression pattern in genes differentially expressed (p < 0,01) in kidney of hypertensive ISIAH and normotensive WAG rats are shown in Figures 1 and 2 .…”

Section: Resultsmentioning

confidence: 99%

“…The functional annotation of the genes differentially expressed in ISIAH and WAG kidney helped to suggest the genetic determinants related to blood pressure control in ISIAH rats. The analysis showed that many genes are working in stress-related mode in hypertensive kidney and the alterations in gene expression are likely related to both pathophysiological and compensatory mechanisms [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Candidate genes in quantitative trait loci associated with absolute and relative kidney weight in rats with Inherited Stress Induced Arterial Hypertension

et al. 2015

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BackgroundThe kidney mass is significantly increased in hypertensive ISIAH rats with Inherited Stress Induced Arterial Hypertension as compared with normotensive WAG rats. The QTL/microarray approach was carried out to determine the positional candidate genes in the QTL for absolute and relative kidney weight.ResultsSeveral known and predicted genes differentially expressed in ISIAH and WAG kidney were mapped to genetic loci associated with the absolute and relative kidney weight in 6-month old F2 hybrid (ISIAHxWAG) males. The knowledge-driven filtering of the list of candidates helped to suggest several positional candidate genes, which may be related to the structural and mass changes in hypertensive ISIAH kidney.In the current study, we showed that all loci found for absolute and relative kidney weight didn't overlap with significant or suggestive loci for arterial blood pressure level. So, the genes differentially expressed in ISIAH and WAG kidneys and located in these QTL regions associated with absolute and relative kidney weight shouldn't substantially influence the BP level in the 6 month-old ISIAH rats. However, in some cases, small effects may be suggested.ConclusionsThe further experimental validation of causative genes and detection of polymorphisms will provide opportunities to advance our understanding of the underlying nature of structural and mass changes in hypertensive ISIAH kidney.

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Differential transcriptional activity of kidney genes in hypertensive ISIAH and normotensive WAG rats

Cited by 10 publications

References 64 publications

Comparative transcriptional profiling of renal cortex in rats with inherited stress-induced arterial hypertension and normotensive Wistar Albino Glaxo rats

Comparative transcriptional profiling of renal cortex in rats with inherited stress-induced arterial hypertension and normotensive Wistar Albino Glaxo rats

The gene-expression profile of renal medulla in ISIAH rats with inherited stress-induced arterial hypertension

Candidate genes in quantitative trait loci associated with absolute and relative kidney weight in rats with Inherited Stress Induced Arterial Hypertension

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