Differential transcription of the HLA-DR genes

Vincent, Rachel; Papa, Ilenia; Clot, J; Eliaou, Jean‐François

doi:10.1016/0198-8859(94)90175-9

Cited by 4 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Significantly, this polymorphism in the mouse is highest in the region of the X and Y boxes, where transcription factors bind; it has been suggested that heterozygosity in this region may confer increased fitness through immunological flexibility (29). The extent to which this mechanism might operate in humans is uncertain; for instance, greater variation has been observed in class II expression on human B cells (30).…”

Section: Resultsmentioning

confidence: 99%

Differential expression of major histocompatibility complex class II genes on murine macrophages associated with T cell cytokine profile and protective/suppressive effects

Baumgart

Moos

Schuhbauer

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Differential expression of major histocompatibility complex class II genes on murine macrophages associated with T cell cytokine profile and protective/suppressive effects

Baumgart

Moos

Schuhbauer

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Thus in humans duplicated HLA DR genes have acquired different expression levels, which are associated with disease progression (34,35). How might gene dimorphism interact with this process?…”

Section: Discussionmentioning

confidence: 99%

Patterned variation in murine MHC promoters

Mitchison¹,

Roes²

2002

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

To compare variation in regulatory and coding DNA, promoter sequences have been obtained from wild-derived mice and laboratory rats. The sequences are from the proximal promoter of the H2Aa, H2Ab, H2Eb, and H2K genes of 24 wild-derived inbred strains and a sample of the corresponding exon 2 sequences and of the RT1.Ba gene of six strains of laboratory rat. They reveal a high level of variation in the mouse MHC class II promoters (H2A and H2E), a low level in MHC class I (H2K), and none in the rat. The variation is pronounced in and around the cAMP response element, a major binding site for modulating promoter activity in response to external stimulation. This finding, together with the different levels of variation in MHC classes I and II, is suggestive of natural selection. However, selection operating via the MHC coding sequences must also contribute, as indicated by the minimal variation in both the MHC class II promoter and coding sequences of the rat. Furthermore CIITA (trans-activator of class II) of the mouse has been reported to have minimal variation in its promoter and none in its coding sequence. Taken together these data suggest that the regulatory and coding sequences undergo coselection. Each of the mouse class II promoters has a pattern of variation that appears to be basically dimorphic, with further variation added by recombination͞mutation. The dimorphic allelic lineages are in marginally detectable linkage disequilibrium with the exon 2 sequences, particularly in H2Aa, thus lending further support to the coevolution hypothesis.

show abstract

“…[6]). Moreover, if the amplification efficiencies of standard and target templates are not the same, but both remain constant during the assay, the calibration curve will be shifted in parallel with an amplitude proportional to the number of PCR cycles (n).…”

Section: Calibration Curve and Quantitative Competitive Pcrmentioning

confidence: 99%

“…[6] only if the amplification efficiencies of target and standard templates are the same along the entire PCR, even when the simulation was run beyond the exponential phase into the saturation phase (Fig. 1).…”

Section: Calibration Curve and Quantitative Competitive Pcrmentioning

confidence: 99%

“…The standard is used to compensate for tube-to-tube variations in amplification efficiency and allows, at least in principle, to extend the PCR beyond the exponential phase into the saturation phase (4). Despite the lack of studies related to the accuracy of the method, it has been widely used for absolute and relative quantification of specific nucleic acid sequences (5)(6)(7). The technical aspects for reliable quantification have been reviewed (8), but our knowledge about the theoretical aspects of the competitive PCR process is still very poor.…”

mentioning

confidence: 99%

See 1 more Smart Citation