Differential Tomato Transcriptomic Responses Induced by Pepino Mosaic Virus Isolates with Differential Aggressiveness

Hanssen, Inge M.; Esse, H. Peter van; Ballester, Ana-Rosa; Hogewoning, Sander W.; Parra, Nelia Ortega; Paeleman, Anneleen; Lievens, Bart; Bovy, Arnaud; Thomma, B.P.H.J.

doi:10.1104/pp.111.173906

Cited by 81 publications

(75 citation statements)

References 75 publications

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“…These results also support an existing notion that SA signaling and JA/ET signaling are antagonistic to each other in response to pathogen infection (An and Mou, 2011;Van der Does et al, 2013). In addition, several genes in biological processes, such as carbon metabolism, metabolite transport, cell wall remodeling, protein synthesis and degradation, and photosynthesis, were altered in a manner similar to those described for dicot virus interactions (Whitham et al, 2003(Whitham et al, , 2006Ascencio-Ibáñez et al, 2008;García-Marcos et al, 2009;Hanssen et al, 2011;Postnikova and Nemchinov, 2012). These include genes encoding glutathione S-transferases, cytochrome P450s, glucosyl hydrolases, heat shock proteins, ribosomal components, UPS proteins, and chlorophyll a/ b binding and photosystem-related proteins (Mandadi and Scholthof, 2012).…”

Section: The Panicovirus Complex: New Findings In Plant Virology Usinsupporting

confidence: 86%

“…Paradoxically, in the molecular genetics era, critical advances in our mechanistic understanding of innate immunity have been made primarily by studying plant pathogenic bacteria and fungi and primarily using dicotyledonous (dicot) hosts. Although there have been studies with viruses such as Tobacco mosaic virus (TMV), Tomato bushy stunt virus (TBSV), Potato virus X (PVX), potyviruses, cucumoviruses, and bromoviruses, almost without exception dicotyledonous plants, primarily Nicotiana benthamiana and Arabidopsis thaliana, were used as experimental hosts (Whitham et al, 2003(Whitham et al, , 2006Ascencio-Ibáñez et al, 2008;García-Marcos et al, 2009;Hanssen et al, 2011;Postnikova and Nemchinov, 2012). Far fewer studies pertain to viruses that infect grasses (the Poaceae).…”

Section: Introductionmentioning

confidence: 99%

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Plant Immune Responses Against Viruses: How Does a Virus Cause Disease?

2013

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Plants respond to pathogens using elaborate networks of genetic interactions. Recently, significant progress has been made in understanding RNA silencing and how viruses counter this apparently ubiquitous antiviral defense. In addition, plants also induce hypersensitive and systemic acquired resistance responses, which together limit the virus to infected cells and impart resistance to the noninfected tissues. Molecular processes such as the ubiquitin proteasome system and DNA methylation are also critical to antiviral defenses. Here, we provide a summary and update of advances in plant antiviral immune responses, beyond RNA silencing mechanisms-advances that went relatively unnoticed in the realm of RNA silencing and nonviral immune responses. We also document the rise of Brachypodium and Setaria species as model grasses to study antiviral responses in Poaceae, aspects that have been relatively understudied, despite grasses being the primary source of our calories, as well as animal feed, forage, recreation, and biofuel needs in the 21st century. Finally, we outline critical gaps, future prospects, and considerations central to studying plant antiviral immunity. To promote an integrated model of plant immunity, we discuss analogous viral and nonviral immune concepts and propose working definitions of viral effectors, effector-triggered immunity, and viral pathogen-triggered immunity.

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Section: The Panicovirus Complex: New Findings In Plant Virology Usinsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Plant Immune Responses Against Viruses: How Does a Virus Cause Disease?

2013

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“…It was shown that all genes encoding enzymes involved in the biosynthesis of lycopene, including geranylgeranyl pyrophosphate synthase, phytoene synthase, phytoene desaturase, z-carotene desaturase, and carotene isomerase, were suppressed upon PepMV infection in tomato seedlings. 35 To study transcriptional gene changes involved in the biosynthesis of b-carotene in response to CMV infection, we tested the effect of CMV on the accumulation of phytoene desaturase (PDS) transcripts, a key-enzyme in b-carotene biosynthesis in tobacco plants. Total RNA from tobacco roots infected with P. aegyptiaca or from tobacco roots infected with both P. aegyptiaca and CMV was extracted 45 d after infection (dpi) and subjected to RT-PCR using specific primers corresponding to PDS and actin genes.…”

Section: Resultsmentioning

confidence: 99%

“…The resulting cDNA was used as a template in a PCR to amplify the PDS gene using the PDSspecific primers 5 0 GTG AAA CAC AAC TCC TTG GC -3 0 (forward) and 5 0 -TCC CTA GTT CTC CAA ACA GG -3 0 (reverse). PCR conditions consisted of 94 C for 4 min, 35 …”

Section: Rna Extraction and Rt-pcr Of The Phytoene Desaturase (Pds) Genementioning

confidence: 99%

Cucumber Mosaic Virusas a carotenoid inhibitor reducingPhelipanche aegyptiacainfection in tobacco plants

Ibdah

Dubey

Eizenberg

et al. 2014

Plant Signaling & Behavior

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Cucumber Mosaic Virus (CMV) is a highly infectious cucumovirus, which infects more than 800 plant species and causes major diseases in greenhouse and field crops worldwide. Parasitic weeds such as Phelipanche aegyptiaca are a major constraint to the production of many crops in the world and the parasite's lifestyle makes control extremely difficult. The parasite seeds can germinate after conditioning and perceiving strigolactones secreted by the host roots. Strigolactones are rhizosphere signaling molecules in plants that are biosynthesized through carotenoid cleavage. In the present study we investigated the possibility of reducing b-carotene and then strigolactone production in the host roots by blocking carotenoid biosynthesis using CMV-infected tobacco. It was found that CMV downregulated the enzyme phytoene desaturase(PDS) and reduced significantly both carotenoid production and Phelipanche infection in tobacco host roots infected with both CMV and P. aegyptiaca. Based on our results (decrease of b-carotene and repression of PDS transcripts in tobacco roots), we hypothesized that the reduction of Phelipanche tubercles and shoots occurred due to an effect of CMV on secondary metabolite stimulators such as strigolacetones. Our study indicated that mass production of the host roots was not affected by CMV; however, most inflorescences of Phelipanche grown on CMV-infected tobacco developed abnormally (deformed shoots and short nodes). Carotenoid biosynthesis inhibitors such as CMV can be used to reduce the production of strigolactones, which will lead to decreased Phelipanche attachment. Interestingly, attenuated CMV strains may provide a safe means for enhancing crop resistance against parasitic weeds in a future plan.

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“…In particular, connections between the interactions of specific virus factors and cell components that cause alterations in hormone synthesis and signaling have been noted (reviewed in references 1 and 2). Recent studies of gene expression profiles in various pathosystems have shown that sets of defense-related genes are expressed upon the infection of susceptible plants with several different viruses (3,4), suggesting that even susceptible plants recognize virus infection and mount defense responses. The discovery of coordinated induction of defense-related genes in compatible plant-virus interactions has led to investigation of the effects of their induced expression on virus accumulation and symptomatology (5,6,7).…”

mentioning

confidence: 99%

Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

García-Marcos

Pacheco

Manzano

et al. 2013

J Virol

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One of the most severe symptoms caused by compatible plant-virus interactions is systemic necrosis, which shares common attributes with the hypersensitive response to incompatible pathogens. Although several studies have identified viral symptom determinants responsible for systemic necrosis, mechanistic models of how they contribute to necrosis in infected plants remain scarce. Here, we examined the involvement of different branches of the oxylipin biosynthesis pathway in the systemic necrosis response caused either by the synergistic interaction of Potato virus X with Potato virus Y (PVX-PVY) or by Tomato spotted wilt virus (TSWV) in Nicotiana benthamiana. Silencing either 9-lipoxygenase (LOX), 13-LOX, or ␣-dioxygenase-1 (␣-DOX-1) attenuated the programmed cell death (PCD)-associated symptoms caused by infection with either PVX-PVY or TSWV. In contrast, silencing of the jasmonic acid perception gene, COI1 (Coronatine insensitive 1), expedited cell death during infection with compatible viruses. This correlated with an enhanced expression of oxylipin biosynthesis genes and dioxygenase activity in PVX-PVY-infected plants. Moreover, the Arabidopsis thaliana double lox1 ␣-dox-1 mutant became less susceptible to TSWV infection. We conclude that oxylipin metabolism is a critical component that positively regulates the process of PCD during compatible plant-virus interactions but does not play a role in restraining virus accumulation in planta.

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Differential Tomato Transcriptomic Responses Induced by Pepino Mosaic Virus Isolates with Differential Aggressiveness

Cited by 81 publications

References 75 publications

Plant Immune Responses Against Viruses: How Does a Virus Cause Disease?

Plant Immune Responses Against Viruses: How Does a Virus Cause Disease?

Cucumber Mosaic Virusas a carotenoid inhibitor reducingPhelipanche aegyptiacainfection in tobacco plants

Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

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