Differential temporal control of <i>Foxa.a</i> and <i>Zic-r.b</i> specifies brain versus notochord fate in the ascidian embryo

Ikeda, Tatsuro; Satou, Yutaka

doi:10.1242/dev.142174

Cited by 22 publications

(19 citation statements)

References 26 publications

(41 reference statements)

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“…b , Dmrt1 , Otx , and Dlx . b play essential roles in the specification of epidermal and neural fates [11, 19, 20, 22–24, 29], Foxd is likely to suppress ectodermal fates in the vegetal hemisphere.…”

Section: Resultsmentioning

confidence: 99%

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Differential gene expression along the animal-vegetal axis in the ascidian embryo is maintained by a dual functional protein Foxd

et al. 2017

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In many animal embryos, a specific gene expression pattern is established along the animal-vegetal axis soon after zygotic transcription begins. In the embryo of the ascidian Ciona intestinalis, soon after the division that separates animal and vegetal hemispheres into distinct blastomeres, maternal Gata.a and β-catenin activate specific genes in the animal and vegetal blastomeres, respectively. On the basis of these initial distinct gene expression patterns, gene regulatory networks promote animal cells to become ectodermal tissues and vegetal cells to become endomesodermal tissues and a part of the nerve cord. In the vegetal hemisphere, β-catenin directly activates Foxd, an essential transcription factor gene for specifying endomesodermal fates. In the present study, we found that Foxd also represses the expression of genes that are activated specifically in the animal hemisphere, including Dmrt1, Prdm1-r.a (Bz1), Prdm1-r.b (Bz2), and Otx. A reporter assay showed that Dmrt1 expression was directly repressed by Foxd, and a chromatin immunoprecipitation assay showed that Foxd was bound to the upstream regions of Dmrt1, Prdm1-r.a, Prdm1-r.b, and Otx. Thus, Foxd has a dual function of activating specific gene expression in the vegetal hemisphere and of repressing the expression of genes that are normally expressed in the animal hemisphere. This dual function stabilizes the initial patterning along the animal-vegetal axis by β-catenin and Gata.a.

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Section: Resultsmentioning

confidence: 99%

“…a and Prdm1-r . b in the neural lineage of the animal hemisphere [11, 19–22]. Animal hemisphere cells that are not induced by Fgf9/16/20 signaling give rise to epidermal cells under the control of Tfap2-r .…”

Section: Introductionmentioning

confidence: 99%

Differential gene expression along the animal-vegetal axis in the ascidian embryo is maintained by a dual functional protein Foxd

et al. 2017

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“…We have previously demonstrated that transcriptional repressors Hes.a and Prdm1-r.a/b delay the onset of the specification program of the brain, and that this delay enables Foxc to be expressed in the ANB 8,45 . If this is indeed involved in emergence of the ANB in the common ancestor of ascidians and vertebrates, Foxg may subsequently be recruited as a downstream factor of Foxc as a specifier for the ANB.…”

Section: Discussionmentioning

confidence: 99%

Foxg specifies sensory neurons in the anterior neural plate border of the ascidian embryo

Liu

Satou

2019

Nat Commun

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Foxg constitutes a regulatory loop with Fgf8 and plays an important role in the development of anterior placodes and the telencephalon in vertebrate embryos. Ascidians, which belong to Tunicata, the sister group of vertebrates, develop a primitive placode-like structure at the anterior boundary of the neural plate, but lack a clear counterpart of the telencephalon. In this animal, Foxg is expressed in larval palps, which are adhesive organs with sensory neurons. Here, we show that Foxg begins to be expressed in two separate rows of cells within the neural plate boundary region under the control of the MAPK pathway to pattern this region. However, Foxg is not expressed in the brain, and we find no evidence that knockdown of Foxg affects brain formation. Our data suggest that recruitment of Fgf to the downstream of Foxg might have been a critical evolutionary event for the telencephalon in the vertebrate lineage.

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“…The DM domain (PF00751 ‐ DNA binding domain) and the related Pfam DMA (PF03474) are essential for the development of anterior neural plate derivatives in tunicates (Tresser et al, ). Forkhead (PF00250) is involved in the specification of neural tissue during ascidian development (Ikeda & Satou, ). Vasohibin (PF14822) belongs to a protein acting as a negative feedback regulator of angiogenesis (Watanabe et al, ).…”

Section: Methodsmentioning

confidence: 99%

Into the bloom: Molecular response of pelagic tunicates to fluctuating food availability

et al. 2019

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The planktonic tunicates appendicularians and thaliaceans are highly efficient filter feeders on a wide range of prey size including bacteria and have shorter generation times than any other marine grazers. These traits allow some tunicate species to reach high population densities and ensure their success in a favourable environment. However, there are still few studies focusing on which genes and gene pathways are associated with responses of pelagic tunicates to environmental variability.Herein, we present the effect of food availability increase on tunicate community and gene expression at the Marquesas Islands (South-East Pacific Ocean). By using data from the Tara Oceans expedition, we show that changes in phytoplankton density and composition trigger the success of a dominant larvacean species (an undescribed appendicularian). Transcriptional signature to the autotroph bloom suggests key functions in specific physiological processes, i.e., energy metabolism, muscle contraction, membrane trafficking, and proteostasis. The relative abundance of reverse transcription-related Pfams was lower at bloom conditions, suggesting a link with adaptive genetic diversity in tunicates in natural ecosystems. Downstream of the bloom, pelagic tunicates were outcompeted by copepods. Our work represents the first metaomics study of the biological effects of phytoplankton bloom on a key zooplankton taxon.

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Differential temporal control of Foxa.a and Zic-r.b specifies brain versus notochord fate in the ascidian embryo

Cited by 22 publications

References 26 publications

Differential gene expression along the animal-vegetal axis in the ascidian embryo is maintained by a dual functional protein Foxd

Differential gene expression along the animal-vegetal axis in the ascidian embryo is maintained by a dual functional protein Foxd

Foxg specifies sensory neurons in the anterior neural plate border of the ascidian embryo

Into the bloom: Molecular response of pelagic tunicates to fluctuating food availability

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