Differential susceptibility to carbapenems due to the AdeABC efflux pump among nosocomial outbreak isolates of Acinetobacter baumannii in a Chinese hospital

Huang, Lei; Sun, Li‐Ying; Xu, Guobing; Xia, Tian

doi:10.1016/j.diagmicrobio.2008.06.008

Cited by 39 publications

(35 citation statements)

References 25 publications

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“…The presence of this genes coding in antibiotic resistance was show in this 150 isolates. Molecular typing of genes that related to A. baumannii antibiotic resistance pattern show the frequency of genes as dfrA1 (63.7%), sul1 (68.6%), aac (3) [27].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance and Carriage Integron Classes in Clinical Isolates of Acinetobacter Baumannii from Isfahan Hospitals, Iran

Nourbakhsh

Rajai

Momtaz

2017

Zahedan J Res Med Sci

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Background: Acinetobacter baumannii is a significant nosocomial pathogen around the world, especially in the intensive care unit that most A. baumannii infections are caused by the outbreak strains. Objectives: This study has been performed in Acinetobacter baumannii isolates, aimed to detect integron classes I, II, III and molecular typing of A. baumannii genes. Methods: In this Cross-sectional study, Acinetobacter baumannii isolated from 150 patients in Isfahan hospitals then antibiotic resistance pattern was determined by disk diffusion method (Kirby Bauer). The presence of genes coding in antibiotic resistance and integrons class I, II, III were analyzed by using of M-PCR method. The data were analyzed by Chi-square, Fischer's test and SPSS statistical software version 16. Results: Antibiotic resistance pattern for Acinetobacter baumannii show that the high resistance was for ciprofloxacin with frequency of 98.3%, ceftazidime with 89.4%, and tetracycline with frequency of 87.3%. The most sensitive antibiotics were chloramphenicol, and nitrofurantoin with frequency of 3.5% and 3.2% resistance. The detection of dfrA1 (63.7%), sul1 (68.6%), aac (3)-IV (54.4%), tet (B) (22.4%), tet (A) (78.3%), aadA1 (15.4%), CITM (17. %), vim (12.2%), Qnr (17.1%), blaSHV (19.8%), sim (7.8%), Oxa-24-like (13.2%), Oxa-51-like (11.9%), Oxa-58-like (39.4%), Oxa-23-like (12.6%), imp (9.2%), cmlA (19%) and cat1 (8.6%) were respectively reported too. Also in this study Frequency of integrons class 1, 2, 3 were (100%), (28%), (6.6%) respectively. Conclusions: High prevalence of integrons among Acinetobater baumannii isolated from Isfahan hospitals indicate the importance role of integron classes in multidrug resistance. Considering the increasing pattern of MDR infections is one of the important issues of treatment which can be effective strategy for curing.

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Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance and Carriage Integron Classes in Clinical Isolates of Acinetobacter Baumannii from Isfahan Hospitals, Iran

Nourbakhsh

Rajai

Momtaz

2017

Zahedan J Res Med Sci

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“…However, the contribution of AdeABC to resistance to meropenem but not to imipenem was observed following inactivation of the pump in OXA-23-producing strains (67). Evidence for AdeABCmediated efflux of carbapenems is further suggested by the correlation between overexpression of the adeB gene and the resistance level in clinical isolates (28,34). A decrease in the imipenem MIC in the presence of CCCP was detected against in vitro mutants and clinical strains (27).…”

Section: Rnd Efflux Systemsmentioning

confidence: 99%

Efflux-Mediated Antibiotic Resistance in Acinetobacter spp

Coyne

Courvalin

Périchon

2011

Antimicrob Agents Chemother

437

376

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Among Acinetobacter spp., A. baumannii is the most frequently implicated in nosocomial infections, in particular in intensive care units. It was initially thought that multidrug resistance (MDR) in this species was due mainly to horizontal acquisition of resistance genes. However, it has recently become obvious that increased expression of chromosomal genes for efflux systems plays a major role in MDR. Among the five superfamilies of pumps, resistance-nodulation-division (RND) systems are the most prevalent in multiply resistant A. baumannii. RND pumps typically exhibit a wide substrate range that can include antibiotics, dyes, biocides, detergents, and antiseptics. Overexpression of AdeABC, secondary to mutations in the adeRS genes encoding a two-component regulatory system, constitutes a major mechanism of multiresistance in A. baumannii. AdeIJK, intrinsic to this species, is responsible for natural resistance, but since overexpression above a certain threshold is toxic for the host, its contribution to acquired resistance is minimal. The recently described AdeFGH, probably regulated by a LysR-type transcriptional regulator, also confers multidrug resistance when overexpressed. Non-RND efflux systems, such as CraA, AmvA, AbeM, and AbeS, have also been characterized for A. baumannii, as have AdeXYZ and AdeDE for other Acinetobacter spp. Finally, acquired narrow-spectrum efflux pumps, such as the major facilitator superfamily (MFS) members TetA, TetB, CmlA, and FloR and the small multidrug resistance (SMR) member QacE in Acinetobacter spp., have been detected and are mainly encoded by mobile genetic elements.

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“…Estas moléculas producen la interrupción del gradiente de protones en la membrana, por lo cual los antimicrobianos no pueden ser expulsados por la bomba AdeABC 2 . Si la presencia del protón-ionóforo produce la disminución de a lo menos 4 veces la CIM del antimicrobiano se considera que existe una contribución del mecanismo de expulsión en la resistencia al agente antibacteriano 35,37 . Además, es importante considerar que la expresión de los genes adeABC se encuentra regulada por un sistema de dos componentes, denominado AdeRS (Figura 3) 31 .…”

Section: Contribución De Adeabc En La Resistenciaunclassified

“…En ese trabajo se informa del aislamiento de una cepa con una mutación puntual en un gen regulador de la expresión de AdeABC, lo que se traduce en la sobreexpresión de la bomba, disminuyendo a la mitad la CIM de meropenem e imipenem, en comparación con los valores de CIM de la cepa salvaje 3,14 . Sin embargo, en el año 2008, Huang y cols 35 , asociaron esta bomba directamente con la resistencia a meropenem. Estos investigadores estudiaron dos cepas multi-resistentes, genéticamente no relacionadas, una de las cuales era productora de la enzima OXA-23, mientras que la otra no sintetizaba esta carbapenemasa.…”

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Bombas de expulsión multidrogas en Acinetobacter baumannii y resistencia a antimicrobianos

C¹,

M²,

Y³

et al. 2009

Rev. chil. infectol.

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Bacterial multi-drugs systems contribute to the development of multi-resistance patterns of Acinetobacter baumannii, a nosocomial pathogen of increasing importance due to its emerging resistance to carbapenems. The multi-resistance phenomena is generated by a combination of mechanisms, one of which the efflux pump system. Many of these multiresistant isolates of A. baumannii harbor genes for the AdeABC multi-drug efflux system, related with resistance to various groups of antibacterial agents, including tygecicline and meropenem. Inhibition of these systems would allow to increase the efficacy of this antimicrobial. This review focuses on the multi-drug efflux pump system of A. baumanni with special emphasis in the AdeABC system.

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Differential susceptibility to carbapenems due to the AdeABC efflux pump among nosocomial outbreak isolates of Acinetobacter baumannii in a Chinese hospital

Cited by 39 publications

References 25 publications

Antibiotic Resistance and Carriage Integron Classes in Clinical Isolates of Acinetobacter Baumannii from Isfahan Hospitals, Iran

Antibiotic Resistance and Carriage Integron Classes in Clinical Isolates of Acinetobacter Baumannii from Isfahan Hospitals, Iran

Efflux-Mediated Antibiotic Resistance in Acinetobacter spp

Bombas de expulsión multidrogas en Acinetobacter baumannii y resistencia a antimicrobianos

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