Differential sulfate uptake by two species of bacteria under light or dark incubation conditions

Monheimer, Richard H.

doi:10.1139/m74-222

Cited by 5 publications

(4 citation statements)

References 5 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initial dissolved inorganic carbon was calculated from field measurements of alkalinity (American Public Health Association et al, 1981). Sulphate uptake was measured by the method of Monheimer (1974Monheimer ( , 1978Monheimer ( , 1979. Artificial substrates (colonized for 3 weeks) were incubated for 6h in 150 ml of streamwater inoculated with 10/iCi of Na^^SOj (New England Nuclear).…”

Section: Sample Collection and Analysismentioning

confidence: 99%

Effects of copper on production of periphyton, nitrogen fixation and processing of leaf litter in a Sierra Nevada, California, stream

Leland

Carter

1985

Freshwater Biology

View full text Add to dashboard Cite

SUMMARY. Production of periphyton, nitrogen fixation and processing of leaf litter were examined in an oligotrophic Sierra Nevada stream and the responses of these processes to copper (2.5, 5 and 10μg 1‐1 CuT [total filtrable copper]; approximately 12, 25 and 50 ng 1‐1 Cu2+) were determined. Autotrophic and total production were estimated from 3‐week accumulations of biomass on artificial substrates. Mean autotrophic production in the control ranged from 0.22 to 0.58 mg C m‐2 h‐1 in summer‐autumn 1979, but declined to 0.08–0.28 mg C m 2 h‐1 after peak discharge in summer 1980, apparently due to phosphorus‐limited growth. Total production in the control ranged from 0.30 to 0.82 mg C m‐2 h ‐1 in summer‐autumn 1979 and from 0.16 to 0,68 mg C m ‐2 h ‐1 in 1980. Mean autotrophic productivity, estimated by l4C‐bicarbonate uptake in daylight, ranged from 0.30 to 2.8 mg C m‐2 h‐1. Autotrophic productivity was reduced by 57–81% at 2.5μg 1‐1 CuT, 55–96% at 5μg 1‐1 CuT, and 81–100% at 10μg 1‐1 CUT, Heterotrophic productivity (based on dark 35S‐sulphate uptake) was inhibited to a lesser extent (28–63% at 2.5μg 1‐1 CuT, 24–84% at 5μg 1‐1 CuT, and 67–92% at 10μg 1‐1 CuT), The inhibition of autotrophic and heterotrophic productivity persisted through the year of exposure. Production in stream sections previously exposed to 2.5 and 5μg 1‐1CuT increased to control levels within 4 weeks after dosing, but remained depressed for more than 7 weeks after exposure to 10μg 1‐1 CuT. The specific rate of photosynthesis (mg C mg chlorophyll a‐1 h‐1) of mature periphyton communities declined at all test concentrations of copper, but the rate for periphyton on newly‐colonized surfaces did not change. The species composition of benthic algae shifted during exposure to an assemblage more tolerant of copper. Achrtanthes minutissima and Fragilaria crotonensis were the primary replacement species on newly‐colonized surfaces. The nitrogenase activity of blue‐green algae was low. with controls ranging from 2.4 to 12 nmol C2H2 m‐2 h‐1. Nitrogenase activity was inhibited during the initial weeks of exposure by 5 and 10μg 1‐1 CuT. However, after 9 months of exposure, control and copper‐treated sections did not differ. The rate of processing of leaf litter, estimated by microbial respiration and nutrient quality of litter of resident riparian woodland taxa, was inhibited at all test concentrations of copper.

show abstract

Section: Sample Collection and Analysismentioning

confidence: 99%

Effects of copper on production of periphyton, nitrogen fixation and processing of leaf litter in a Sierra Nevada, California, stream

Leland

Carter

1985

Freshwater Biology

View full text Add to dashboard Cite

show abstract

“…Since 28-h cultures of these bacteria are in exponential growth (9), high rates of sulfur uptake by such cultures would be expected. The 28-h cultures of C. striatum and P. fluorescens in the factorial experiments did take up sulfate (the only source of sulfur) the fastest.…”

Section: Discussionmentioning

confidence: 99%

“…Corynebacterium striatum, Pseudomonas fluorescens, and Serratia marcescens, species that might be expected to be present in natural aquatic ecosystems, are capable of growth in this defined medium and were used as the experimental organisms. The particular strains of bacteria used in this study have been identified and characterized previously (9).…”

mentioning

confidence: 99%

Effects of three environmental variables on sulfate uptake by aerobic bacteria.

Monheimer¹

1975

Applied Microbiology

View full text Add to dashboard Cite

The effects of various concentrations of sulfate, organic sulfur, and organic carbon on sulfate uptake by aerobic bacteria were studied using pure cultures growing in a defined medium. Cultures of Pseudomonas fluorescens and Corynebacterium striatum took up sulfate faster when young, but sulfate uptake by Serratia marcescens was faster in older cultures. Organic sulfur was found to decrease sulfate uptake, but at concentrations somewhat higher than occurs in most natural freshwater ecosystems. Low levels of sulfate can theoretically directly limit bacterial biomass production but such limitation probably does not occur in natural systems. Evidence is presented which indirectly links the uptake of sulfate and organic carbon, adding credibility to the proposal that sulfate uptake can be used as an indicator of microbial biomass production in freshwater ecosystems.

show abstract

“…Sulfate, for example, has long been recognized as a major nutrient, but the relationship(s) between light and sulfate uptake by aerobic microplankton is (are) relatively unstudied. Some aerobic heterotrophic bacteria have been shown to take up sulfate faster in the dark than in light (Monheimer, 1974a), while some algae have been shown to take up sulfate in complete darkness (Monheimer, 1978). Data from physiological studies of algae have shown that many of the mechanisms for transporting substrates into the cells, including those for sulfate uptake, are more active at higher rates of photosynthesis (Robinson,969a,b).…”

mentioning

confidence: 99%

Effect of light intensity on sulfate uptake and primary productivity by natural freshwater microplankton communities and axenic algal cultures

Monheimer

1981

Hydrobiologia

Self Cite

View full text Add to dashboard Cite

show abstract

Differential sulfate uptake by two species of bacteria under light or dark incubation conditions

Cited by 5 publications

References 5 publications

Effects of copper on production of periphyton, nitrogen fixation and processing of leaf litter in a Sierra Nevada, California, stream

Effects of copper on production of periphyton, nitrogen fixation and processing of leaf litter in a Sierra Nevada, California, stream

Effects of three environmental variables on sulfate uptake by aerobic bacteria.

Effect of light intensity on sulfate uptake and primary productivity by natural freshwater microplankton communities and axenic algal cultures

Contact Info

Product

Resources

About