Differential Spectrophotometric Method for Determination of Florfenicol

Abstract

Simple, selective and accurate differential first derivative spectrophotometric method (∆D 1) was developed for the determination of florfenicol in bulk and pharmaceutical formulation. The developed method was based on measuring the ∆D 1 of florfenicol in alkaline solutions against its aqueous solutions as blank. Beer's law was found to be linear over the concentration range 3-15 µg/mL with good correlation coefficient (0.999). The selectivity of the method was proved through freedom from interferences.

An eco-friendly first and second derivative synchronous spectrofluorimetry for quantification of florfenicol in presence of its different degradation products. Application to kinetic stability study

“…Pathways of alkaline and acidic degradation of FLR have been investigated before [ 32 ], where the degradation products were separated and identified by liquid chromatography–mass spectrometry [ 32 ]. By referring to electronic supplementary material, scheme S1, it is obvious that hydrolysis of the amide linkage is the main route of both alkaline and acidic degradation [ 33 ], which is followed by defluorination in alkaline degradation producing the hydroxyl derivative instead [ 32 ]. Since the resultant degradation products are more polar than the parent drug [ 30 ], it is expected that their prevalence in surface water is significant.…”