Differential signaling involved in Sutherlandia frutescens-induced cell death in MCF-7 and MCF-12A cells

Vorster, C.J.J.; Stander, André; Joubert, Annie M.

doi:10.1016/j.jep.2011.12.045

Cited by 16 publications

(9 citation statements)

References 23 publications

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“…Because this species has a strong reputation in folklore as a medicinal plant that treats cancers, studies to validate these claims are plenty. For example, some studies showed that extracts lead to apoptosis of breast cancer cell lines with MCF-7 tumor cells being more susceptible to cell death in comparison to the MCF-12 A cells [7,8]. They also inhibit growth of CaCo 2 colon cancer cells by disrupting cell membranes and cell death is mediated via the P13-K pathway [9].…”

Section: Introductionmentioning

confidence: 99%

The Implication of Chemotypic Variation on the Anti-Oxidant and Anti-Cancer Activities of Sutherlandia frutescens (L.) R.Br. (Fabaceae) from Different Geographic Locations

et al. 2020

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Extracts of Sutherlandia frutescens (cancer bush) exhibit considerable qualitative and quantitative chemical variability depending on their natural wild origins. The purpose of this study was thus to determine bioactivity of extracts from different regions using in vitro antioxidant and anti-cancer assays. Extracts of the species are complex and are predominantly composed of a species-specific set of triterpene saponins (cycloartanol glycosides), the sutherlandiosides, and flavonoids (quercetin and kaempferol glycosides), the sutherlandins. For the Folin-Ciocalteu phenolics test values of 93.311 to 125.330 mg GAE/g DE were obtained. The flavonoids ranged from 54.831 to 66.073 mg CE/g DE using the aluminum chloride assay. Extracts from different sites were also assayed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging method and ferric reducing anti-oxidant power (FRAP) methods. This was followed by an in vitro Cell Titer-Glo viability assay of various ecotypes using the DLD-1 colon cancer cell line. All test extracts displayed anti-oxidant activity through the DPPH• radical scavenging mechanism, with IC50 values ranging from 3.171 to 7.707 µg·mL−1. However, the degree of anti-oxidant effects differed on a chemotypic basis with coastal plants from Gansbaai and Pearly Beach (Western Cape) exhibiting superior activity whereas the Victoria West inland group from the Northern Cape, consistently showed the weakest anti-oxidant activity for both the DPPH• and FRAP methods. All extracts showed cytotoxicity on DLD-1 colon cancer cells at the test concentration of 200 µg·mL−1 but Sutherlandia plants from Colesburg (Northern Cape) exhibited the highest anti-cancer activity. These findings confirm that S. frutescens specimens display variability in their bioactive capacities based on their natural location, illustrating the importance of choosing relevant ecotypes for medicinal purposes.

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Section: Introductionmentioning

confidence: 99%

The Implication of Chemotypic Variation on the Anti-Oxidant and Anti-Cancer Activities of Sutherlandia frutescens (L.) R.Br. (Fabaceae) from Different Geographic Locations

et al. 2020

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“…[12][13][14][15][16] Its ethanolic extract has been reported to induce a concentration-dependent anti-proliferative activity on human tumours including the SNO oesophageal cancer and MCF-7 breast cancer cell lines. 17,18 Similarly, its aqueous extract was also reported to induce cytotoxicity in both MCF-7 and MDAMB 231 breast cancer cell lines as well as in HL60 and Jurkat leukaemia cells. [18][19][20][21][22] More so, S. frutescens has been reported to show anti-cancer activity in prostate cancer as it induced cytotoxicity in human prostate cancer cells DU-145, PC3, LNCaP and mouse prostate cancer cell, TRAMP-C2.…”

Section: Introductionmentioning

confidence: 97%

“…17,18 Similarly, its aqueous extract was also reported to induce cytotoxicity in both MCF-7 and MDAMB 231 breast cancer cell lines as well as in HL60 and Jurkat leukaemia cells. [18][19][20][21][22] More so, S. frutescens has been reported to show anti-cancer activity in prostate cancer as it induced cytotoxicity in human prostate cancer cells DU-145, PC3, LNCaP and mouse prostate cancer cell, TRAMP-C2. 20,23 It also induced apoptosis and inhibited the PI3K-kinase cell survival pathway in CaCo2 colon cancer cells.…”

Section: Introductionmentioning

confidence: 97%

Preliminary Cytotoxic Activity of Sutherlandia frutescens and Carpobrotus edulis on Malignant glioblastoma Cells

Omoruyi¹,

Enogieru²,

Ekpo³

2019

TJNPR

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Glioblastoma (GBM) is the most common and aggressive intracranial tumour with limited therapeutic options due to their high tumour vasculature and invasiveness. Treatment of GBM includes surgery, followed by chemotherapy and radiotherapy. Despite these treatment options, tumour relapse is still a major issue hence the need for cheap and effective treatment strategies. South Africa is endowed with numerous medicinal plants including Sutherlandia frutescens (S. frutescens) and Carpobrotus edulis (C.edulis), which has been previously reported for their antioxidant and neuroprotective activities. Accordingly, this study was designed to investigate the cytotoxicity of both medicinal plants (S. frutescens and C. edulis) in malignant human GBM cells U251 and U87. The cytotoxic activity was measured using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay while the effect of extracts on colony formation and survival was determined using clonogenic assay. Results show that both S. frutescens and C .edulis induced cytotoxicity in both GBM cells and inhibit colony formation in U251 cells. These findings show that extracts from these plants may be useful in the treatment of GBM and thus requires further investigations into their mechanisms of action as well as isolation of bioactive components responsible for these activities.

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“…Sutherlandia frutescens, commonly known as the "cancer bush" by South African healers, is a medicinal plant traditionally used to treat many human diseases, including cancer (Van Wyk et al, 2002;Prevoo et al, 2004;Grandi et al, 2005;Chadwick et al, 2007;Van Wyk and Albrecht, 2008;Van Wyk, 2011). Ethanolic extract of S. frutescens showed dose-dependent anti-proliferative and apoptotic effects on several human tumors including the breast cancer cell line MCF-7 (Vorster et al, 2012). A similar inhibition can also be achieved by aqueous extract (Tai et al, 2004;Steenkamp and Gouws, 2006;Stander et al, 2009;Vorster et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…frutescens showed dose‐dependent anti‐proliferative and apoptotic effects on several human tumors including the breast cancer cell line MCF‐7 (Vorster et al, ). A similar inhibition can also be achieved by aqueous extract (Tai et al, ; Steenkamp and Gouws, ; Stander et al, ; Vorster et al, ). Chinkwo () demonstrated that an aqueous extract induced cellular apoptosis in cervical carcinoma and Chinese hamster ovary (CHO) cells.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Gli/hedgehog signaling in prostate cancer cells by “cancer bush” Sutherlandia frutescens extract

Lin

Jackson

et al. 2015

Cell Biology International

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Sutherlandia frutescens is a medicinal plant traditionally used to treat various types of human diseases, including cancer. Previous studies of several botanicals link suppression of prostate cancer growth with inhibition of the Gli/hedgehog (Gli/Hh) signaling pathway. Here we hypothesized the anti-cancer effect of S. frutescens was linked to its inhibition of the Gli/Hh signaling in prostate cancer. We found a dose- and time- dependent growth inhibition in human prostate cancer cells PC3 and LNCaP, and mouse prostate cancer cells TRAMP-C2 treated with S. frutescens methanol extract (SLE). We also observed a dose-dependent inhibition of the Gli-reporter activity in Shh Light II and TRAMP-C2QGli cells treated with SLE. In addition, SLE can inhibit Gli/Hh signaling by blocking Gli1 and Ptched1 gene expression in the presence of a Gli/Hh signaling agonist (SAG). A diet supplemented with S. frutescens suppressed the formation of poorly differentiated carcinoma in prostates of TRAMP mice. Finally, we found Sutherlandioside D was the most potent compound in the crude extract that could suppress Gli-reporter in Shh Light II cells. Together this suggests that the S. frutescens extract may exert anti-cancer effect by targeting Gli/Hh signaling, and Sutherlandioside D is one of the active compounds.

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Differential signaling involved in Sutherlandia frutescens-induced cell death in MCF-7 and MCF-12A cells

Cited by 16 publications

References 23 publications

The Implication of Chemotypic Variation on the Anti-Oxidant and Anti-Cancer Activities of Sutherlandia frutescens (L.) R.Br. (Fabaceae) from Different Geographic Locations

The Implication of Chemotypic Variation on the Anti-Oxidant and Anti-Cancer Activities of Sutherlandia frutescens (L.) R.Br. (Fabaceae) from Different Geographic Locations

Preliminary Cytotoxic Activity of Sutherlandia frutescens and Carpobrotus edulis on Malignant glioblastoma Cells

Inhibition of Gli/hedgehog signaling in prostate cancer cells by “cancer bush” Sutherlandia frutescens extract

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