Differential Sensitivity of Well-Differentiated Avian Respiratory Epithelial Cells to Infection by Different Strains of Infectious Bronchitis Virus

Rahman, Sahar Abd El; Winter, Christine; El-Kenawy, Aly; Neumann, Ulrich; Herrler, Georg

doi:10.1128/jvi.00463-10

Cited by 19 publications

(24 citation statements)

References 8 publications

(14 reference statements)

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“…Pretreatment with neuraminidase was found to result in decreased infectivity as indicated by a reduced number of infected cells and by lower titers of virus released into the supernatant. This finding was obtained with both a lab strain and strains circulating in poultry [124][125][126]. The sialic acid-dependence of the IBV infection was observed both with conventional cell cultures and differentiated airway epithelial cells from trachea and lung [126,127].…”

Section: Gammacoronavirusesmentioning

confidence: 61%

Sialic Acid Receptors of Viruses

Matrosovich

Herrler

Klenk

2013

Topics in Current Chemistry

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232

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Sialic acid linked to glycoproteins and gangliosides is used by many viruses as a receptor for cell entry. These viruses include important human and animal pathogens, such as influenza, parainfluenza, mumps, corona, noro, rota, and DNA tumor viruses. Attachment to sialic acid is mediated by receptor binding proteins that are constituents of viral envelopes or exposed at the surface of non-enveloped viruses. Some of these viruses are also equipped with a neuraminidase or a sialyl-O-acetyl-esterase. These receptor-destroying enzymes promote virus release from infected cells and neutralize sialic acid-containing soluble proteins interfering with cell surface binding of the virus. Variations in the receptor specificity are important determinants for host range, tissue tropism, pathogenicity, and transmissibility of these viruses.

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Section: Gammacoronavirusesmentioning

confidence: 61%

Sialic Acid Receptors of Viruses

Matrosovich

Herrler

Klenk

2013

Topics in Current Chemistry

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232

262

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“…Reports about infection studies with PCLS are rare and restricted to the murine, bovine, and avian lungs [10], [11], [17], [18]. In this study, PCLS prepared from porcine lungs were shown to be a valuable culture system for porcine differentiated respiratory epithelial cells.…”

Section: Discussionmentioning

confidence: 79%

Infection of Differentiated Porcine Airway Epithelial Cells by Influenza Virus: Differential Susceptibility to Infection by Porcine and Avian Viruses

et al. 2011

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BackgroundSwine are important hosts for influenza A viruses playing a crucial role in the epidemiology and interspecies transmission of these viruses. Respiratory epithelial cells are the primary target cells for influenza viruses.Methodology/Principal FindingsTo analyze the infection of porcine airway epithelial cells by influenza viruses, we established precision-cut lung slices as a culture system for differentiated respiratory epithelial cells. Both ciliated and mucus-producing cells were found to be susceptible to infection by swine influenza A virus (H3N2 subtype) with high titers of infectious virus released into the supernatant already one day after infection. By comparison, growth of two avian influenza viruses (subtypes H9N2 and H7N7) was delayed by about 24 h. The two avian viruses differed both in the spectrum of susceptible cells and in the efficiency of replication. As the H9N2 virus grew to titers that were only tenfold lower than that of a porcine H3N2 virus this avian virus is an interesting candidate for interspecies transmission. Lectin staining indicated the presence of both α-2,3- and α-2,6-linked sialic acids on airway epithelial cells. However, their distribution did not correlate with pattern of virus infection indicating that staining by plant lectins is not a reliable indicator for the presence of cellular receptors for influenza viruses.Conclusions/SignificanceDifferentiated respiratory epithelial cells significantly differ in their susceptibility to infection by avian influenza viruses. We expect that the newly described precision-cut lung slices from the swine lung are an interesting culture system to analyze the infection of differentiated respiratory epithelial cells by different pathogens (viral, bacterial and parasitic ones) of swine.

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“…For human work, ALI cultures are an alternative if the tissue samples are limited, but human lung slices can be commonly obtained from surgical specimens and have been used to examine infection with adenovirus type 7 [103] or with anthrax spores [104]. Avian lung slices infected with infectious bronchitis virus for a week displayed an infection pattern based on strain and innoculum dose [105]. This distribution appeared to match the distribution or expression of the sialic-acid based receptor sites, detected with lectin, on the epithelial cells and used by the virus for infection.…”

Section: Pathophysiology In Lung Slicesmentioning

confidence: 99%

Exploring lung physiology in health and disease with lung slices

Sanderson

2011

Pulmonary Pharmacology & Therapeutics

141

159

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The development of therapeutic approaches to treat lung disease requires an understanding of both the normal and disease physiology of the lung. Although traditional experimental approaches only address either organ or cellular physiology, the use of lung slice preparations provides a unique approach to investigate integrated physiology that links the cellular and organ responses. Living lung slices are robust and can be prepared from a variety of species, including humans, and they retain many aspects of the cellular and structural organization of the lung. Functional portions of intrapulmonary airways, arterioles and veins are present within the alveoli parenchyma. The dynamics of macroscopic changes of contraction and relaxation associated with the airways and vessels are readily observed with conventional low-magnification microscopy. The microscopic changes associated with cellular events, that determine the macroscopic responses, can be observed with confocal or two-photon microscopy. To investigate disease processes, lung slices can either be prepared from animal models of disease or animals exposed to disease invoking conditions. Alternatively, the lung slices themselves can be experimentally manipulated. Because of the ability to observe changes in cell physiology and how these responses manifest themselves at the level of the organ, lung slices have become a standard tool for the investigation of lung disease.

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Differential Sensitivity of Well-Differentiated Avian Respiratory Epithelial Cells to Infection by Different Strains of Infectious Bronchitis Virus

Cited by 19 publications

References 8 publications

Sialic Acid Receptors of Viruses

Sialic Acid Receptors of Viruses

Infection of Differentiated Porcine Airway Epithelial Cells by Influenza Virus: Differential Susceptibility to Infection by Porcine and Avian Viruses

Exploring lung physiology in health and disease with lung slices

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