Differential sensitivity of three marine invertebrates to copper assessed using multiple biomarkers

Brown, Rebecca; Galloway, T.S.; Lowe, DM; Browne, Mark A. Oakley; Dissanayake, Awantha; Jones, M. B.; Depledge, Michael H.

doi:10.1016/j.aquatox.2003.10.001

Cited by 233 publications

(92 citation statements)

References 47 publications

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“…An increase of MTs following mussel exposure to Cu was previously experimentally obtained (Roesijadi et al 1988), but the relationship between MTs and Cu does not have to be so evident at low levels of Cu exposure (Langston et al 1998). For example, the synthesis of MTs in whole soft tissue of M. edulis was not induced even after seven days of exposure to 68.1 g Cu L -1 (Brown et al 2004). In this study, Cu was not determined in the seawater of selected sampling sites, but the reported Cu concentrations in marinas (total Cu: 10.3 g L -1 ; Cmuk 2005) and small ports (total Cu: 11.4-15.0 g L -1 ; Cmuk 2005; Louis et al 2008) in the nearby area, indicate to the low exposure of mussels to Cu in the studied area.…”

Section: Metal Levels In the Heat-treated Cytosolmentioning

confidence: 95%

Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue

Dragun

Erk

Ivanković

et al. 2009

Environ Sci Pollut Res

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Dragun, Zrinka; Erk, Marijana; Ivanković, Dušica; Žaja, Roko; Filipović Marijić, Vlatka; Raspor, Biserka. Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue. // Environmental Science and Pollution Research. 17 (2010) , 4; 977-986 Dragun, Zrinka; Erk, Marijana; Ivanković, Dušica; Žaja, Roko; Filipović Marijić, Vlatka; Raspor, Biserka. Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue. // Environmental Science and Pollution Research. 17 (2010) , 4; 977-986. 2 Abstract Purpose: The aim of this study was to compare the level of metal contamination in two bays in the middle part of the Eastern Adriatic coastal zone in Croatia using the gills of mussels Mytilus galloprovincialis as indicator tissue. Despite the existing sources of contamination, previous studies with caged mussels only indicated moderate metal contamination of the Kaštela Bay, contrary to the Trogir Bay in which marina and shipyard present a probable source of Cu-and Zn-contamination.Methods: The measurements of metallothioneins (MTs) and metals that induce MT synthesis (Cu, Zn and Cd) were performed in the heat-treated gill cytosol, and total proteins (TPs) in the untreated gill cytosol. MTs were determined by differential pulse voltammetry, Cu and Zn by flame atomic absorption spectrometry (AAS), Cd by graphite furnace AAS and TPs by Bradford spectrophotometric procedure.Results: The results collected in four sampling campaigns (autumn periods from [2001][2002][2003][2004] indicated that MT levels in mussel gills (expressed on dry mass basis 2.30.3 mg g -1 ) were comparable with basal levels reported in the literature (2.50.8 mg g -1 ). Observed interindividual, temporal and spatial MT variability could be associated with different confounding factors, such as the time of sampling, total protein concentration and mussel size rather than cytosolic levels of Cu and Zn. Metal levels, expressed on wet mass basis, in the heat-treated gill cytosol ranged from 1.33-11.31 g g -1 for Zn, from 0.72-2.96 g g -1 for Cu and from 0.036-0.100 g g -1 for Cd.The highest Zn level was measured at Vranjic (Kaštela Bay) -the site influenced by untreated domestic wastewater, while somewhat increased Zn and the highest Cu levels were found at marina and shipyard locations (Trogir Bay). The highest Cd level was measured at Inavinil (Kaštela Bay).Conclusions: The observed association of gill MT levels with several biotic and abiotic factors, limits its use as the biomarker of low-level metal exposure. Therefore, the use of the metal concentrations in the heat-treated gill cytosol of Mediterranean mussels should be considered for the assessment of the low-level metal contamination of coastal marine areas.

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Section: Metal Levels In the Heat-treated Cytosolmentioning

confidence: 95%

Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue

Dragun

Erk

Ivanković

et al. 2009

Environ Sci Pollut Res

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“…Thus, haemolymph glucose level alteration cannot withstand alone as bioindicator of copper stress response, but it should be evaluated in combination with other more specific biomarkers, like lysosomal membrane stability in this study. Understanding how different biomarkers relate to each other on exposure to particular contaminants is a key to interpreting the effects of biomarkers in the field (Brown et al 2004). In conclusion, significant membrane destabilisation of C. aestuarii blood cells lysosomes in response to copper toxicity could be a very good biomarker of effects and it could be used successfully in field biomonitoring programmes for early detection of cellular damages.…”

Section: Discussionmentioning

confidence: 99%

“…Exposure concentration of copper was chosen with reference to the published sublethal data on biomarker responses for Carcinus species (Brown et al 2004), which was 70 µg/L as described previously (Lazo et al 2004;Arapi et al 2008).…”

Section: Test Solutionsmentioning

confidence: 99%

Copper Induced Lysosomal Membrane Destabilisation in Haemolymph Cells of Mediterranean Green Crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania)

Aliko

Hajdaraj

Caci

et al. 2015

Braz. arch. biol. technol.

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“…Haemolymph (40 µl) was transferred onto a glass slide cells were allowed to adhere in a humidity chamber (15 mins) before incubating (15 min) with the neutral red dye. Cells were observed under a high powered microscope over time (15, 30, 60, 75, 90 120, 150, 190 min) and the retention of the dye within the haemocyte lysosomes was recorded (as employed by Brown et al, 2004).…”

Section: Shore Crab Physiological Assessmentmentioning

confidence: 99%

Monitoring PAH contamination in the field (South west Iberian Peninsula): Biomonitoring using fluorescence spectrophotometry and physiological assessments in the shore crab Carcinus maenas (L.) (Crustacea: Decapoda)

Dissanayake¹,

Bamber²

2010

Marine Environmental Research

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Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants of the marine environment, arising predominantly from petrochemical contamination and pyrogenic sources. A biomarker of PAH exposure was employed in a field study (South West, Spain) in both captured (indigenous) and deployed (caged) shore crabs (Carcinus maenas) in the chronic PAH-exposed Bays of Algeciras and Gibraltar (from associated harbour and boating activity) compared to a relatively "clean" site (Cadiz).Metabolite fluorescence was attributed to the following key priority PAH groups; naphthalenes (NAPs), pyrenes (PYRs) and benzo[a]pyrenes (BAPs). Temporal variability was assessed using deployed populations over an eight week period.Petrogenic and pyrogenic PAH contamination (as an indicator of the PAH type) was demonstrated using a ratio between FF BAP+PYR / FF NAP . Physiological assessments from deployed crabs demonstrated both physiological and cellular alterations as shown by reduced heart rates (at rest) and increased cellular stress in crabs from the PAH contaminated sites.

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Differential sensitivity of three marine invertebrates to copper assessed using multiple biomarkers

Cited by 233 publications

References 47 publications

Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue

Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue

Copper Induced Lysosomal Membrane Destabilisation in Haemolymph Cells of Mediterranean Green Crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania)

Monitoring PAH contamination in the field (South west Iberian Peninsula): Biomonitoring using fluorescence spectrophotometry and physiological assessments in the shore crab Carcinus maenas (L.) (Crustacea: Decapoda)

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