Differential scanning microcalorimetry study of the thermal denaturation of haemoglobin

Michnik, Anna; Drzazga, Z.; Kluczewska, Aneta; Michalik, Katarzyna

doi:10.1016/j.bpc.2005.06.012

Cited by 40 publications

(19 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The first approaches of different diseases by DSC method were published a few years ago [14][15][16][17]. These important papers published that inflammatory and oncological diseases revealed significant changes in the plasma curve with a shift of the curve toward higher denaturation temperatures, whereas studies have indicated that these changes are resulted from interactions of small molecules or peptides with these proteins [24,36]. But, the exact background of the marked differences in the thermal denaturation profile is not yet known.…”

Section: Discussionmentioning

confidence: 95%

Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

Zapf

Moezzi

Fekecs³

et al. 2015

J Therm Anal Calorim

View full text Add to dashboard Cite

Damage caused by oxidative stress is involved in many types of diseases, including breast cancer. Our aim was to detect the oxidative stress parameters and blood plasma changes with differential scanning calorimetry (DSC) in breast cancer patients. The study included 40 adult breast cancer women who were grouped according to tumor diameter, regional lymph node metastases, proliferative activity, receptor status and postoperative chemotherapy. To monitor oxidative stress, malondialdehyde, oxygen free radicals (OFRs), activity of myeloperoxidase (MPO), superoxide dismutase (SOD) and catalase (CAT) were measured. Denaturation of plasma components was detected in Setaram Micro DSC-II calorimeter. The total production of OFRs, the MPO activity and lipidperoxidation were significantly increased in each breast cancer patients considering the tumor size, the metastatic lymph nodes, the proliferation activity and receptor status compared with healthy controls (p \ 0.05). These pro-oxidants were slightly elevated without chemotherapy, but their values were increased significantly in chemotherapy-receiving group. The activity of SOD and CAT was significantly decreased in all groups, and in regard to the chemotherapy, they were changed significantly parallel to the severity of disease. Regarding to both the increased tumor diameter and the increased number of affected lymph nodes, DSC measurements showed a strong relationship between the maximum excess heat capacity (C pmax ) of the blood plasma and the severity of disease. The study demonstrated that oxidative stress is implicated in breast carcinoma and chemotherapy aggravates these changes which confirmed the plasma DSC measurements also.

show abstract

Section: Discussionmentioning

confidence: 95%

Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

Zapf

Moezzi

Fekecs³

et al. 2015

J Therm Anal Calorim

View full text Add to dashboard Cite

show abstract

“…The application of heat is a wellestablished method that allows protein aggregation to occur on a biochemically feasible time scale. In fact, the thermally induced unfolding of many proteins has been shown to be occasionally followed by an irreversible process that induces aggregation (Sanchez-Ruiz et al 1988;Azuaga et al 2002;Rezaei et al 2002;Weijers et al 2003;Michnik et al 2005). Generally, such aggregation has been modeled as shown in Scheme 1, where N, U, and A are native, unfolded, and irreversible unfolded protein forms, respectively (Lumry and Eyring 1954;Sanchez-Ruiz et al 1988;Azuaga et al 2002;Rezaei et al 2002;Weijers et al 2003;Michnik et al 2005).…”

Section: Thermally Induced Aggregation Of Proteinsmentioning

confidence: 99%

Application and use of differential scanning calorimetry in studies of thermal fluctuation associated with amyloid fibril formation

Sasahara

Goto

2012

Biophys Rev

View full text Add to dashboard Cite

The aggregation of proteins into amyloid fibrils is a topic that has attracted great interest because the process is associated with the pathology of numerous human diseases. Despite considerable progress in the elucidation of the structure of amyloid fibrils and the kinetic mechanism of their formation, knowledge on the thermodynamic aspects underlying the formation and stability of amyloid fibrils is limited. In this review, we summarize recent calorimetric studies of amyloid fibril formation, with the goal of obtaining a better understanding of the causal factors that thermally induce proteins to aggregate into amyloid fibrils. Calorimetric data show that differential scanning calorimetry is a useful technique to study the causative factors that thermally trigger the conversion to the amyloid structure and highlight the physics related to the thermal fluctuation of proteins during this conversion.

show abstract

“…However, the main maximum of DSC runs occurring in temperature above 65°C can be mainly affected by ab and c globulin fractions [18]. Additionally the denaturation process in this range can be slightly influenced by the haemoglobin forms [19] due to the contamination of the rat's blood serum as can be seen in UV-VIS spectra (Fig. 1).…”

Section: Resultsmentioning

confidence: 95%

Calorimetric and spectroscopic studies characterization of newborn rat’ blood serum after maternal administration of cyclophosphamide

Drzazga

Michalik

Halat

et al. 2010

J Therm Anal Calorim

Self Cite

View full text Add to dashboard Cite

Differential scanning microcalorimetry (DSC) and UV-VIS absorption spectroscopy were used to obtain the characteristics of blood serum from newborn rat' after maternal treatment with cyclophosphamide in comparison with control. The obtained DSC curves reveal a complex endothermic peak due to the unfolding process of various serum proteins. Thermal profiles and absorption spectra of blood serum are sensitive to the age of newborns as well as to effect of maternal administration of cyclophosphamide. The most significant disturbances in serum proteome were observed for 14-day old newborns. The thermodynamic parameters: enthalpy change (DH), the normalized first moment (M 1 ) of the thermal transition with respect to the temperature axis and the ratio of C p ex at 70 and 60°C describing denaturation contributions of globulin forms in respect to unliganded albumin with haptoglobin was estimated. Moreover, the second derivative spectroscopy in the UV region was used to resolve the complex protein spectrum. The differences in blood serum detected by DSC and UV-VIS confirm a potential usefulness of these methods for diagnostic and monitoring changes with age as well as the pathological state of blood serum.

show abstract

Differential scanning microcalorimetry study of the thermal denaturation of haemoglobin

Cited by 40 publications

References 27 publications

Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

Application and use of differential scanning calorimetry in studies of thermal fluctuation associated with amyloid fibril formation

Calorimetric and spectroscopic studies characterization of newborn rat’ blood serum after maternal administration of cyclophosphamide

Contact Info

Product

Resources

About