Differential scanning calorimetry study on caprylins

Silva, Sara Anunciação Braga; Araújo, Maria Cláudia Este De; Ract, Juliana Neves Rodrigues; Vítolo, Michele

doi:10.1007/s10973-015-4409-3

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…However, the decreasing of AV s was not proportional to the dilution of caprylic acid, since the AV s for the 1:1 GLY:CA blend (206.5 mg NaOH/g) is 2.3 times higher than for 5:1 blend (89.6 mg NaOH/g) instead of three times. Probably, a fraction of caprylic acid molecules were not available to neutralization by NaOH due to intermolecular interactions with glycerol molecules by weak chemical bonds such as hydrogen bond (Silva et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Feasibility on the Use of Acid Value for Evidencing the Esterification of Glycerol with Caprylic Acid Catalyzed by Lipase

Vítolo¹,

Ract²,

Guebara³

2017

AJS

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The present paper deals with the use of Acid Value (AV), a titration method largely used for fatty acid quantification, to determine the acidity of products resulting from caprylic acid (CA) and excess glycerol (GLY) esterification catalyzed by an immobilized lipase, aiming to obtain caprylins (CL), as well as the blends of these two components (GLY/CA) in different proportions used as substrate for the reactions. Response surface methodology (RSM) was used to determine the effects of three variables (temperature, reaction time, and GLY/CA molar ratio) on the caprylin production optimization. The AV of the blends decreased with an increasing molar ratio GLY/CA and a coefficient variation of 11% for the AV method was obtained. The RSM did not provide a clear statistical correlation between AV and the reaction parameters selected as variables. However, among all obtained AV values, 77% were in the range between 50 and 100 mg NaOH/g, including the experiment performed under the conditions of 60 o C, 8h, and GLY/CA molar ratio of 2, in which the highest caprylin production yield of about 50% was obtained.

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Section: Discussionmentioning

confidence: 99%

Feasibility on the Use of Acid Value for Evidencing the Esterification of Glycerol with Caprylic Acid Catalyzed by Lipase

Vítolo¹,

Ract²,

Guebara³

2017

AJS

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“…Similarly, crystallization peak for 83% C 10 -DAG was at lower temperature than that of 99% C 10 -DAG, recording peaks at 15.55 and 22.12 • C, respectively. It was postulated that this phenomenon was attributed to the presence of TAG in the MCD samples of lower purity, causing the reduction in the crystallization temperature as TAG formed crystals by van der Waals force instead of hydrogen bonds (Silva et al, 2015). In terms of the melting curve, the 80% C 8 -DAG showed melting behavior at −23.62 • C and was completely melted at 9.76 • C while 99% C 8 -DAG FIGURE 3 | (A) Crystallization and (B) melting curves of C 8 -DAG and C 10 -DAG after single-and double-step purification.…”

Section: Thermodynamic Properties Andmentioning

confidence: 99%

Immobilized Lipase in the Synthesis of High Purity Medium Chain Diacylglycerols Using a Bubble Column Reactor: Characterization and Application

Chen

Lee

Qiu

et al. 2020

Front. Bioeng. Biotechnol.

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Novozym R 435, an immobilized lipase from Candida antarctica B. (CALB), was used as a biocatalyst for the synthesis of high purity medium chain diacylglycerol (MCD) in a bubble column reactor. In this work, the properties of the MCD produced were characterized followed by determining its practical application as an emulsifier in water-in-oil (W/O) emulsion. Two types of MCDs, namely, dicaprylin (C 8-DAG) and dicaprin (C 10-DAG), were prepared through enzymatic esterification using the following conditions: 5% Novozym R 435, 2.5% deionized water, 60 • C for 30 min followed by purification. A singlestep molecular distillation (MD) (100-140 • C, 0.1 Pa, 300 rpm) was performed and comparison was made to that of a double-step purification with MD followed by silica gel column chromatography technique (MD + SGCC). Crude C 8-DAG and C 10-DAG with DAG concentration of 41 and 44%, respectively, were obtained via the immobilized enzyme catalyzing reaction. Post-purification via MD, the concentrations of C 8-DAG and C 10-DAG were increased to 80 and 83%, respectively. Both MCDs had purity of 99% after the MD + SGCC purification step. Although Novozym R 435 is a nonspecific lipase, higher ratios of 1,3-DAG to 1,2-DAG were acquired. Via MD, the ratios of 1,3-DAG to 1,2-DAG in C 8-DAG and C 10-DAG were 5.8:1 and 7.3:1, respectively. MCDs that were purified using MD + SGCC were found to contain 1,3-DAG to 1,2-DAG ratios of 8.8:1 and 9.8:1 in C 8-DAG and C 10-DAG, respectively. The crystallization and melting peaks were shifted to higher temperature regions as the purity of the MCD was increased. Dense needle-like crystals were observed in MCDs with high purities. Addition of 5% C 8-DAG and C 10-DAG as emulsifier together in the presence of 9% of hydrogenated soybean oil produced stable W/O emulsion with particle size of 18 and 10 µm, respectively.

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“…Isto pode ser explicado pelo efeito da temperatura na atividade enzimática e afinidade da enzima pelo substrato(EOM et al, 2010). A elevação ideal de Em outras palavras, se a formação de 1,3-DAGs foi favorecida em relação à de 1,2-DAGsa Lipozyme de R. miehei é de especificidade sn,1-3então a hidroxila da posição 2 do 1,3-DAG tornar-se-ia menos disponível à esterificação do que a hidroxila da posição 3 do 1,2-DAG(SILVA, et al, 2015).Umas das propriedades mais características das enzimas imobilizadasdaí a sua importânciaé a possibilidade de recuperação e reuso do biocatalisador. Portanto, foi testado o reciclo da Lipozyme RM IM ® em reações do tipo batelada em reator descontínuo convencional e em reator munido de membrana de microfiltração.…”

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Reação entre glicerol e ácido caprílico catalisada por lipase imobilizada

Guebara¹

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Differential scanning calorimetry study on caprylins

Cited by 5 publications

References 19 publications

Feasibility on the Use of Acid Value for Evidencing the Esterification of Glycerol with Caprylic Acid Catalyzed by Lipase

Feasibility on the Use of Acid Value for Evidencing the Esterification of Glycerol with Caprylic Acid Catalyzed by Lipase

Immobilized Lipase in the Synthesis of High Purity Medium Chain Diacylglycerols Using a Bubble Column Reactor: Characterization and Application

Reação entre glicerol e ácido caprílico catalisada por lipase imobilizada

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