Differential scanning calorimetry of the irreversible denaturation of Rapana thomasiana (marine snail, Gastropod) hemocyanin

Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins

“…The first step is a reversible unfolding of the native protein (N). This is followed by an irreversible change of the denatured protein (D) into a final irreversible state (I) [44,45]. …”

“…The irreversibility of BPP denaturation was investigated by a multiple reheating experiment, according to the method proposed by by Idakieva and Michnik [45,60]. From the initial DSC scan, we have determined the values of the transition temperatures at 107°C, 145 °C and 156 °C for glucose, sucrose and inulin at 10% w/v, respectively (Table 5).…”

“…In this case, the denaturation process can be regarded as one-step process following first-order kinetics [44][45][46], (Eq.5).…”

Purification of Hemocyanin from Marine GastropodRapana Thomasianausing Ammonium Sulfate Precipitation Method

“…new hc has been isolated and purified from the hemolymph of this marine gastropod using ultracentrifugation and column chromatography (6). Rapana thomasiana hemocyanin (Rth) has been structurally well characterized (2,3,6,7,8,9,10,12,14,15,16). Recently, we demonstrated the high immunogenicity of Rth as a model antigen and also its properties as a strong protein carrier for viral peptides from Influenza hemagglutinin (17).…”

Estabilidade térmica da hemoglobina extracelular gigante de Glossoscolex paulistus (HbGp): estudos dos efeitos do pH do meio e do estado de oxidação do ferro por microcalorimetria diferencial de varredura (DSC), espectroscopia de absorção óptica e dicroísmo circular (CD)

“…A Microcalorimetria diferencial de varredura (DSC) é uma técnica usada amplamente no monitoramento das variações de energia envolvidas nos processos de desnaturação de macromoléculas biológicas [31][32][33][34][35][36][37][38][39][40][41].…”