Differential Scanning Calorimetry (DSC): A Tool to Study the Thermal Behavior of Lipid Bilayers and Liposomal Stability

Demetzos, Costas

doi:10.1080/08982100802310261

Cited by 263 publications

(155 citation statements)

References 14 publications

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“…In order to provide better contrast, uranyl acetate staining was used. DSC studies were carried out to determine the chain melting temperature of the liposome bilayer 34 using a differential scanning calorimeter DSC 1, STAR e system, Mettler Toledo, Columbus, OH, USA with a scan rate of 2 / min. The temperature range was set to 15-65 for SPC, DPPC, DPPG, and DPPC DPPG liposomes.…”

Section: Instrumentationmentioning

confidence: 99%

Influence of Lipid Composition, pH, and Temperature on Physicochemical Properties of Liposomes with Curcumin as Model Drug

et al. 2016

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Section: Instrumentationmentioning

confidence: 99%

Influence of Lipid Composition, pH, and Temperature on Physicochemical Properties of Liposomes with Curcumin as Model Drug

et al. 2016

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“…This happens because of the hydrophobic tail C 12 H 25 -, which penetrates into the bilayer and causes membrane disruption and phase separation. As a result, they exhibit properties similar to those of ordinary (isotropic) liquids [14,20]. However, a transition peak around 54°C was observed for each thermogram, which is the main transition temperature for HSPC lipids, something which is expected, as long as there is still conformation among phospholipids.…”

Section: Thermotropic Behavior Investigation Of Hspc:c 12 H 25 -Pnipamentioning

confidence: 84%

“…Finally, the slight hysteresis in the cooling diagrams of both conventional and chimeric systems, around 3°C, possibly suggests the formation of a partial interdigitated phase [20].…”

Section: Thermotropic Behavior Investigation Of Hspc:c 12 H 25 -Pnipamentioning

confidence: 99%

Innovative drug nanocarriers by incorporating thermoresponsive polymer in phospholipid bilayers

Demetzos¹,

Tzani²,

Naziris³

et al. 2017

Glob Drugs Therap

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“…52 Figure 11 represents our phase transition temperature profiles of PEGylated lipids detected by means of the DSC method. It can be seen that the DSC phase transition profiles exhibit intense and well-defined sharp peaks for both lipids.…”

Section: Differential Scanning Calorimetry Thermogram Of Pegylated LImentioning

confidence: 99%

Vibrational spectroscopic studies of newly developed synthetic biopolymers

2010

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Vibrational spectroscopic techniques such as near‐infrared (NIR), Fourier transform infrared (FTIR), and Raman spectroscopy are valuable diagnostic tools that can be used to elucidate comprehensive structural information of numerous biological samples. In this review article, we have highlighted the advantages of nanotechnology and biophotonics in conjunction with vibrational spectroscopic techniques in order to understand the various aspects of new kind of synthetic biopolymers termed as polyethylene glycol (PEG)ylated lipids. In contrast to conventional phospholipids, these novel lipids spontaneously form liposomes or nanovesicles upon hydration, without the supply of external activation energy. The amphiphiles considered in this study differ in their hydrophobic acyl chain length and contain different units of PEG hydrophilic headgroups. We have further explored the thermotropic phase behaviors and associated changes in the conformational order/disorder of such lipids by using variable‐temperature FTIR and Raman spectroscopy. Phase transition temperature profiles and correlation between various spectral indicators have been identified by either monitoring the shifts in the vibrational peak positions or plotting vibrational peak intensity ratios in the CH stretching region as a function of temperature. To supplement our observations of phase transformations, a thermodynamic approach known as differential scanning calorimetry (DSC) has been applied and revealed a good agreement with the infrared and Raman spectroscopic data. Finally, the investigation of thermal properties of lipids is extremely crucial for numerous purposes, thus the results obtained in this work may find application in a wide variety of studies including the development of PEGylated lipid based drug and substances delivery vehicles. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 403–417, 2010.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Differential Scanning Calorimetry (DSC): A Tool to Study the Thermal Behavior of Lipid Bilayers and Liposomal Stability

Cited by 263 publications

References 14 publications

Influence of Lipid Composition, pH, and Temperature on Physicochemical Properties of Liposomes with Curcumin as Model Drug

Influence of Lipid Composition, pH, and Temperature on Physicochemical Properties of Liposomes with Curcumin as Model Drug

Innovative drug nanocarriers by incorporating thermoresponsive polymer in phospholipid bilayers

Vibrational spectroscopic studies of newly developed synthetic biopolymers

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