Differential Salinity Tolerance in Calli and Shoots of Four Rice Cultivars

Rattana, Khwanduean; Bunnag, Sumontip

doi:10.3923/ajcs.2015.48.60

Cited by 12 publications

(6 citation statements)

References 22 publications

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“…In the same manner, the increased concentration of saline in the cultural medium had a significant role in reducing regeneration rate, where the salinity level of 14 dS m -1 showed the minimum regeneration rate for both cultivars. These results are stated in previous studies on several crops species, including rice (17,20), wheat (5), sugarcane (15) and peas (19).…”

Section: Regeneration Of Plantlets From Nacl Tolerant Callisupporting

confidence: 87%

Molecular evaluation of salt tolerance induced by sodium azide in immature embryos of two wheat cultivars

Mahmood¹,

Al-falahi²,

Hamedullah³

et al. 2019

JoBRC

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Background: The artificial induction of mutations represents an efficient tool in creating genetic variations. For this reason, the current study was carried out in the Plant Tissue Culture Laboratory/Genetic Engineering Institute to induce genetic diversity for salt tolerance in two local cultivars of bread wheat (Al-Iraq and Tamooz 2) using in vitro application of sodium azide (SA). Objective: Immature seeds from both cultivars subjected to 0, 0.5, 1.0 and 2.0 Mm of SA to determine the optimal concentration for developing novel mutants. Materials and methods: The optimal dose of SA mutagen was found to be 2.0 mM, resulting in a 42% reduction in callus fresh and dry weight. The developed callus was subjected to five different salinity levels using NaCl (6, 8, 10, 12 and 14 dS m-1). Results: Mutants showed a significant decrease in the percentage of regenerated plants under salinity stress conditions. The used SSR markers approved the genetic diversity between the original and the mutants of the two cultivars (Al-Iraq and Tamooz 2) growing under normal and salinity stress. The presences and the absence of some fragments was prominent in plants derived from immature embryos of the two cultivars tested in salinity conditions. The used SSR markers (cfd 9, cfd4, cfd1 wmc405, PYL5, HKT1, HVA1 and htk1) were so efficient in distinguishing between the original and tissue culture-derived plants. Furthermore, the experienced SA levels induced a higher rate of mutant alleles in cv. Conclusion: Al-Iraq with 19 mutant alleles than Tamooz 2 which showed only 13 mutant alleles. The current study represents an additional prove to the effectiveness of mutagens and tissue culture technique in developing novel variants with improved performance under stress conditions.

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Section: Regeneration Of Plantlets From Nacl Tolerant Callisupporting

confidence: 87%

Molecular evaluation of salt tolerance induced by sodium azide in immature embryos of two wheat cultivars

Mahmood¹,

Al-falahi²,

Hamedullah³

et al. 2019

JoBRC

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“…In vitro culture, a tissue culture approach is frequently identified as a salt-tolerant crop and was employed to find salt-tolerant cell lines of rice (30,31), barley (32), potato (7,33), sugarcane (34) and tomato (35). Key criteria for success in the in vitro selection technique for salt tolerance are believed to be a large variety of cells, easy salt administration and an in vitro selection approach for the tolerant cell.…”

Section: Introductionmentioning

confidence: 99%

Hydroponic and in vitro screening of wheat varieties for salt-tolerance

et al. 2022

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Salt-tolerant wheat cultivars are essential for sustainable wheat production and global food security. The present study aimed to establish a reliable screening protocol as well as successfully isolated the potential salt-tolerant wheat varieties by discerning morpho-physiological parameters with multivariate analysis. Seventeen wheat varieties were evaluated at 0, 12, 15 and 18 dSm-1 salinity levels in a hydroponic culture system at the seedling stage. Moreover, in vitro callusing responses of four selected varieties were determined to clarify the salt tolerance capability at 0, 9, 12 and 15 dSm-1 salt treatments. The seedling growth of most wheat varieties was highly interrupted and reduced by the toxic effects of salinity, however, some varieties such as BARI Gom-32, BARI Gom-33, BARI Gom-31, BARI Gom-30, and BARI Gom-28 showed the lowest reduction under all salinity stress conditions. The total salt tolerance index (TSTI) showed that the cultivar BARI Gom-33 was the most salt-tolerant followed by BARI Gom-32 and BARI Gom-30 whereas BARI Gom-25 was identified as the most sensitive. These results were strongly supported by the principal component analysis (PCA) and Ward’s Methods Euclidean based clustering. In vitro results revealed that the lowest reduction of callus induction was recorded in BARI Gom-33 which might show the greatest tolerance to salinity by improving morpho-physiological characteristics against salt stress. Therefore, the identified genotypes might be employed as donor parents to develop salt-tolerant and high-yielding cultivars in the wheat breeding programme.

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“…These studies can also provide information on growth potential and physiological and biochemical responses to NaCl stress at various tissue levels [ 55 ]. Therefore, rice callus culture and shoot regeneration responses to salt stress are critical factors in improving rice salt tolerance [ 56 ]. Numerous reports about callogenesis and adventitious shoot regeneration of Indica rice have been published, but this investigation focused on the diverse rice cultivar Luem Pua, with an in vitro evaluation of calli under various salinity levels.…”

Section: Resultsmentioning

confidence: 99%

Salinity Stress Response of Rice (Oryza sativa L. cv. Luem Pua) Calli and Seedlings

2022

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Soil salinity limits plant growth and production. This research investigated a suitable medium for callus induction and plantlet regeneration in the Luem Pua rice cultivar. The effect of salt stress on seedling growth was determined using in vitro culture and soil conditions. An efficient protocol for callus induction has been developed by culture sterilized seeds on the Murashige and Skoog (MS, 1962) medium containing 0.5 mg/l benzyladenine (BA) with 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) that resulted in a 100% callus induction. Plantlet regeneration percentage of 49% was recorded on the MS medium containing 4 mg/l BA with 0.5 mg/l 1-naphthaleneacetic acid (NAA) after 4 weeks. For salt stress investigation, the calli were treated on an induction medium containing various concentrations of NaCl (0, 50, 100, 150, and 200 mM), while two-week-old rice seedlings were planted in soil and treated with the same concentration of NaCl for 4 weeks. In vitro culture revealed that callus survival percentage decreased when NaCl concentration increased, similar to soil culture. Seedling growth under salinity treatment also decreased when NaCl concentration increased, while other physiological parameters such as total chlorophyll, chlorophyll a, chlorophyll b, green intensity, and chlorophyll fluorescence under light conditions increased under salinity stress. These changes define the growth and physiological salinity tolerance characteristics of Luem Pua rice calli and seedlings. They can be utilized as a baseline for demand-driven in vitro rice propagation, providing useful information that can be combined with other agronomic features in rice development or breeding programs to improve the flexibility of abiotic stress-tolerant cultivars.

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Differential Salinity Tolerance in Calli and Shoots of Four Rice Cultivars

Cited by 12 publications

References 22 publications

Molecular evaluation of salt tolerance induced by sodium azide in immature embryos of two wheat cultivars

Molecular evaluation of salt tolerance induced by sodium azide in immature embryos of two wheat cultivars

Hydroponic and in vitro screening of wheat varieties for salt-tolerance

Salinity Stress Response of Rice (Oryza sativa L. cv. Luem Pua) Calli and Seedlings

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